Phase II study of trastuzumab with modified docetaxel, cisplatin, and 5 fluorouracil in metastatic HER2-positive gastric cancer

Abstract

Background:

Trastuzumab with cisplatin and fluoropyrimidine is the standard treatment in metastatic HER2-positive gastric or gastroesophageal (GE) junction adenocarcinoma, however there is limited data on the efficacy of trastuzumab in combination with a three-drug regimen in this setting. We examined the efficacy and safety of modified docetaxel, cisplatin and 5 fluorouracil (mDCF) plus trastuzumab in a single-arm multicenter phase II trial.

Methods:

Previously untreated patients with HER2-positive metastatic gastric or GE junction adenocarcinoma were treated with mDCF and trastuzumab every 2 weeks. The primary endpoint was 6-month progression free survival (PFS); secondary endpoints included objective response rate, overall survival (OS), and toxicity.

Results:

We enrolled 26 patients with metastatic HER2-positive gastric or GE junction adenocarcinoma between February 2011 and June 2015. The median age of patients was 62 years; 96% had a Karnofsky performance status equal to or greater than 80%. With a median follow-up of 25.4 months, the 6-month PFS was 73% (95% CI, 51% - 86%). The objective response rate was 65%, the median PFS was 13 months (95% CI, 6.4 – 20.7) and the median OS was 24.9 months (95% CI, 14.4 – 42.5). Grade 3/4 toxicities included neutropenia (42%), fatigue (23%), and hypophosphatemia (15%). There were no episodes of febrile neutropenia.

Conclusion:

The combination of mDCF and trastuzumab is effective and safe in patients with metastatic HER2-positive gastric or GE junction adenocarcinoma and can be considered as an option for selected patients. This trial is registered at ClinicalTrials.gov, number .

Mini abstract

The combination of mDCF and trastuzumab is effective and safe in patients with metastatic HER2-positive gastric cancer and can be considered as an option in fit patients.

Introduction

Patients with metastatic gastric cancer (mGC) and gastroesophageal (GE) junction adenocarcinoma are treated with palliative chemotherapy consisting of platinum and fluoropyrimidine, which improves survival and quality of life [1]. Adding a third drug, most commonly a taxane, has shown greater efficacy but is also associated with increased toxicity [2].

A significant fraction (20–25%) of mGCs overexpress the receptor tyrosine kinase HER2 often as a result of amplification of its gene, ERBB2, and this receptor has been validated as a molecular target in the metastatic setting [3]. HER2 amplification is particularly frequent in the chromosomal instability subtype of gastric cancer and frequently co-occurs with TP53 mutations [4]. The HER2-blocking antibody trastuzumab has thus been combined with cisplatin and fluoropyrimidine to treat HER2-positive advanced gastric or GE junction adenocarcinoma; the ToGA trial showed that improvement in overall survival is particularly pronounced in patients with HER2 immunohistochemistry (IHC) 3+ or HER2 IHC 2+ and FISH-positive tumors [5, 6]. Since that trial, trastuzumab has been shown effective and safe in combination with other 2-drug chemotherapy regimens such as XELOX, FOLFOX, and S1 plus cisplatin as first-line therapy [7–12].

The combination of trastuzumab and docetaxel or paclitaxel has demonstrated synergy in HER2-overexpressing human gastric cancer xenograft models [13]. Recently we reported that in patients with mGC modified docetaxel, cisplatin, and 5 fluorouracil (mDCF) was tolerable with similar efficacy as parent DCF [14]. When trastuzumab was approved for HER2 amplified metastatic gastric cancer, the mDCF protocol was amended in order to allow HER2-positive patients to receive this biologic with mDCF, as a separate and independent cohort. Therefore, the aim of this study is to describe the efficacy of mDCF with trastuzumab as measured by 6-month PFS as well as its safety profile in HER2-positive mGC patients.

Methods

Patients

This is a single-arm multicenter phase II trial for patients with metastatic gastric or gastroesophageal (GE) junction adenocarcinoma found to be HER2-positive, defined as IHC staining intensity rated as 3+ on a scale of 0 to 3+ or IHC 2+ with ERBB2 gene amplification confirmed by FISH. Inclusion criteria were radiographically evaluable disease, Karnofsky performance status ≥ 70%, and adequate organ function defined as: hemoglobin ≥ 9.0 g/dL, absolute neutrophil count ≥ 1,500 cells/L, platelet count ≥ 100,000/L, serum creatinine ≤ 1.5 mg/dL, total bilirubin ≤ 1.5 mg/dL, AST, ALT, and alkaline phosphatase within eligible ranges (Online Resource 1) and baseline left ventricular ejection fraction ≥ 50%. In patients with serum creatinine of 1.2 to 1.5 mg/dL, creatinine clearance (calculated or observed) must have been ≥ 50 mL/min. Anticoagulation was allowed. Patients were excluded if they had received prior chemotherapy for metastatic or unresectable disease, received prior docetaxel or cisplatin in the perioperative or adjuvant setting. Other exclusion criteria were: brain or central nervous system metastasis, significant cardiovascular disease (unstable angina, myocardial infarction, stroke, or cerebrovascular accident within 6 months of study entry), or clinically significant hearing loss or tinnitus. Cancer diagnose within the past 3 years (excluding basal cell carcinoma of skin, cervical carcinoma in situ, or non-metastatic prostate cancer) was also an exclusion criterion. All patients signed written informed consent prior to study enrollment. This trial is registered at ClinicalTrials.gov ().

This study protocol was approved by the institutional review boards at Memorial Sloan Kettering Cancer Center (MSKCC) and City of Hope Cancer Center. The study was monitored quarterly by the MSKCC Data and Safety Monitoring Board and MSKCC Therapeutic Response Review Committee. This study was done in accordance with the principles of the Declaration of Helsinki.

Treatment plan and tumor assessment

Patients were treated with mDCF and trastuzumab and the specific modifications to parent DCF were: a de Gramont schedule for 5 fluorouracil (5-FU) administration; reduced bi-monthly doses of cisplatin and docetaxel; the administration of docetaxel and cisplatin on consecutive days instead of immediately following one another. The regimen consists in docetaxel 40 mg/m² day 1, cisplatin 40 mg/m² day 2, leucovorin 400 mg/m² day 1, 5-FU 400 mg/m² day 1, 5-FU 1000 mg/m²/d (continuous intravenous infusion) days 1 and 2, on a 2-week cycle. Trastuzumab was also administered on day 1 of the 2-week cycle, with an initial loading dose of 6 mg/kg in cycle 1 and 4 mg/kg thereafter. Cisplatin was recommended for up to 12 cycles and the rest of the regimen until disease progression, unacceptable toxicity or withdrawal of consent. Tumor growth was followed radiographically by CT of the chest, abdomen, and pelvis, performed every 6 weeks initially and then every 12 weeks after 9 months on therapy. Response and progression were evaluated using Response Evaluation Criteria in Solid Tumors (RECIST) criteria [15].

Toxicity evaluation and dose attenuation

All adverse events were graded according to the National Cancer Institute Common Toxicity Criteria for Adverse Events (v 3.0). Doses were reduced following a standard scale in case of adverse events. Left ventricular function was assessed by echocardiogram every 12 weeks while on study, and trastuzumab was stopped if a patient developed symptomatic congestive heart failure (CHF), or any asymptomatic decrease in left ventricular ejection fraction (LVEF) below 50% with an absolute reduction of at least 10% from baseline.

Statistics

The primary endpoint of this study was 6-month progression-free survival (PFS). PFS was measured from the start of treatment to the date of either documented disease progression or death, whichever occurred first. Given that the 6-month PFS of the parent DCF regimen is approximately 43% [2], we estimated using exact single-stage binomial design that a sample size of 35 HER2-positive patients would be sufficient to differentiate between 6-month PFS of 43% and 64% with type I and II error rates of 10% each [16]. The modified DCF plus trastuzumab regimen would be considered promising if 19 out of 35 patients were alive and progression free at 6 months. We employed an early stopping rule for safety as previously described [14]. We defined an acceptable grade 3 to 4 adverse event rate of 50% and an unacceptable grade 3 to 4 adverse event rate of 70% in the first 3 months of therapy. Other secondary endpoints included response rate, median PFS, and overall and 1- and 2-year survival. The median PFS and median overall survival (OS) were estimated using the Kaplan-Meier method, and 95% confidence intervals were based on the sign test [17]. All statistical analyses were performed using SAS version 9.3 (SAS Institute, INC., Cary, NC, USA). All P-values were two sided. P-values of <0.05 were considered to indicate statistical significance.

Sequencing and Bioinformatics

Participants enrolled after February 2014 were eligible to consent to prospective tumor genomic profiling using the next generation sequencing (NGS) platform MSK-IMPACT. This assay was conducted as previously described [18, 19] and was performed in a CLIA-certified laboratory, using a 341-gene panel. MSK-IMPACT detects mutations, small insertions and deletions, copy number alterations and selected structural rearrangements. We also assessed tumors for microsatellite instability (MSI) using the MSI-sensor method, and samples with a score ≥ 10 were classified as MSI-high [20]. All samples were collected for analysis before starting anti-HER2 treatment. The log-rank test was used to test for associations between specific genomic alterations and PFS.

Results

Patients

Twenty-six patients with HER2-positive metastatic gastric or GE junction adenocarcinoma were enrolled between February 2011 and June 2015. Two patients were enrolled at a collaborative site and the rest were treated at MSKCC. The study was closed before reaching estimated sample size because of slow accrual. The median age at the time of enrollment was 62 years (range, 35 to 77 years) and 25 patients (96%) had a Karnofsky performance status equal to or greater than 80% (Table 1). In this cohort 18 patients’ tumors (69%) qualified as HER2-positive based on IHC 3+ and 8 (31%) based on IHC 2+ in combination with FISH (+). A focal staining pattern was described in 2 patients (13%) with IHC scores of 3+ and in 3 (38%) with 2+.

Table 1.

Baseline patient characteristics (n = 26)

	No. (%)
Age, years*	62 (35–77)
Sex
Male	23 (88.5)
Female	3 (11.5)
Stage at diagnosis
Stage I/II/III	3 (11.5)
Stage IV	23 (88.5)
Tumor location
GE junction	15 (58)
Stomach	11 (42)
Baseline KPS (%)
≥80%	25 (96)
70%	1 (4)
Lauren classification
Intestinal	13 (50)
Mixed	4 (15)
Diffuse	7 (27)
Unknown	2 (8)
HER2 status
IHC 3+	18 (69)
IHC 2+/FISH(+)	8 (31)

Abbreviation: CI, comorbidity index; KPS, Karnofsky performance status.

^*Median (range).

Treatment and outcomes

All 26 patients received at least 1 cycle of mDCF and trastuzumab; the median number of chemotherapy cycles was 21 (range, 1 to 153). The mean relative dose intensities for docetaxel, cisplatin, bolus 5-FU, infusional 5-FU and trastuzumab were 69%, 50%, 90%, 93%, and 100%, respectively. In the intention-to-treat population, the objective response rate was 65% (95% CI, 46% - 80%) (Table 2); response is quantified as best percentage change in tumor diameters in Figure 1. Two patients experienced clinical deterioration or death prior to evaluation by imaging. The median follow-up was 25.4 months; at 6 months, 19 of 26 patients were alive and progression-free, which represents a 6-month PFS of 73% (95% CI, 51 – 86). The median PFS was 13 months (95% CI, 6.4 – 20.7) and the median OS was 24.9 months (95% CI, 14.4 – 42.5) (Figure 2). The 1- and 2-year survival was 73% and 56%, respectively. Patients received second, third and fourth lines of systemic treatment in 81%, 58%, and 12% of cases, respectively. Four patients (15%) were later treated with immune checkpoint inhibitors and 14 (54%) received trastuzumab after progression. Subsequent systemic therapies are listed in Table 3.

Table 2.

Response rate according to RECIST criteria

Best response	n (%)	Duration (months)
Complete response	1 (4)	77
Partial response	16 (62)	19
Stable disease	6 (23)	6
Disease progression	1 (4)	(−)
No further evaluation*	2 (8)	(−)

^*Two patients experienced clinical deterioration/death prior to evaluation by imaging.

Figure 1.

Treatment response of each patient, quantified as best percentage change in sum of longest tumor diameters of target lesions. * One patient with a non-target 14-mm lymph node (short axis) was included in this analysis.

Figure 2.

Outcomes in patients treated with first-line mDCF and trastuzumab. (a) Progression-free survival (PFS) and (b) overall survival (OS).

Table 3.

Subsequent lines of therapy

	n (%)
Second-line therapy
Fluoropyrimidines	4 (15)
Irinotecan	11 (42)
Platin*	10 (38)
Taxane	3 (11.5)
Paclitaxel-ramucirumab	0
Checkpoint inhibitors	3 (11.5)
Trastuzumab	12 (46)
Other	3 (11.5)
Third-line therapy
Fluoropyrimidines	6 (23)
Irinotecan	4 (15)
Platin*	9 (35)
Taxane	2 (8)
Paclitaxel-ramucirumab	1 (4)
Checkpoint inhibitors	2 (8)
Trastuzumab	8 (31)
Other	1 (4)
Fourth-line therapy
Fluoropyrimidines	1 (4)
Irinotecan	0
Platin*	1 (4)
Taxane	0
Paclitaxel-ramucirumab	1 (4)
Checkpoint inhibitors	0
Trastuzumab	2 (8)
Other	1 (4)

^*Includes cisplatin, carboplatin and oxaliplatin

Genomic analysis

The tumors of 11 patients were prospectively genomically profiled at a mean sequencing coverage of 828x. Figure 3 provides a summary of mutations detected by targeted NGS. Sequenced samples were from primary tumors in 8 patients and metastases in 3. All samples were classified as microsatellite stable (MSI score <10). The most frequently altered genes were TP53, in 11 patients (100%), and CDKN2A, in 3 patients (27%). NGS confirmed ERBB2 amplification in 6 patients (55%) and the remaining 5 patients had significantly shorter PFS compared to patients with ERBB2 amplified tumors (median PFS, 11 vs. 30 months, p=0.0072). Among these 5 patients, HER2 staining was only focally positive in 1, and FISH amplification was borderline positive in 2 (Online Resource 2).

Figure 3.

Genomic analysis of advanced HER2-positive gastric cancer. Oncoprint depicts alterations in selected genes of interest in 11 HER2-positive gastric cancer patients treated with mDCF and trastuzumab. Correlation with progression-free survival (a) and overall survival (b) are described.

Toxicity

Grade 3/4 non-hematologic toxicities included fatigue (23%), hypersensitivity reaction (11.5%), dehydration (11.5%), diarrhea (8%), and gastrointestinal hemorrhage (8%). Three patients (11.5%) came off study because of toxicity, two because of grade 3 gastrointestinal bleeding and one because of persistent grade 2 anorexia in the setting of controlled disease. Grade 3/4 neutropenia occurred in 11 patients (42%). One patient (4%) had asymptomatic drop in LVEF below 50% and none had symptomatic CHF. There were no episodes of febrile neutropenia or drug-related mortality. Attributable adverse events are listed in Table 4.

Table 4.

Toxicity possibly, probably, or definitely related to mDCF and trastuzumab (n = 26)

	Any Grade n (%)	Grade 3–4 n (%)
Non-hematologic
Fatigue	13 (50)	6 (23)
Hypophosphatemia	4 (15)	4 (15)
Hypokalemia	5 (19)	3 (11.5)
Hyponatremia	6 (23)	1 (4)
Hyperglycemia	7 (27)	1 (4)
Hypersensitivity reaction	8 (31)	3 (11.5)
Dehydration	5 (19)	3 (11.5)
Diarrhea	10 (38)	2 (8)
Gl hemorrhage	2 (8)	2 (8)
Anorexia	3 (11.5)	1 (4)
Hyperbilirubinemia	3 (11.5)	1 (4)
Hypoalbuminemia	2 (8)	1 (4)
Epiphora	7 (27)	1 (4)
Nausea	11 (42)	1 (4)
Syncope	1 (4)	1 (4)
Thromboembolism	2 (8)	1 (4)
Hematologic
Neutropenia	16 (62)	11 (42)
Lymphopenia	8 (31)	8 (31)
Anemia	17 (65)	7 (27)
Thrombocytopenia	13 (50)	1 (4)

Abbreviation: GI, gastrointestinal.

Discussion

After the seminal ToGA trial, the combination of two chemotherapy drugs and trastuzumab became the standard of care in HER2-amplified mGC. In this study we found that in selected patients, the combination of taxane, fluoropyrimidine, and cisplatin with trastuzumab is also feasible. Furthermore, we observed a promising activity of this regimen with a 6-month PFS of 73%. Notably, grade 3/4 toxicity was low and there were no cases of febrile neutropenia. This trial is a continuation of a randomized phase II trial of mDCF vs. original DCF in unselected mGC [14]. Our findings further support the efficacy of this regimen and reveal no new safety concerns. Other small series also have shown encouraging results with the combination of trastuzumab with a three-drug regimen, such as docetaxel, cisplatin and S1 [21], and docetaxel, oxaliplatin, capecitabine, bevacizumab [22]. In our cohort 96% of patients had KPS ≥80% which may have allowed patients to tolerate treatment for a longer period of time (median number of cycles 21) leading to a better than expected PFS. Remarkably, 81% and 58% of patients received second- and third-line systemic therapy, respectively, suggesting that in appropriately selected patients this regimen can maximize exposure to active treatments.

Our exploratory sequencing analysis failed to confirm ERBB2 amplification in 5 of 11 patients and that group derived limited benefit from trastuzumab-based therapy. This discordance could be attributed to intra-tumor heterogeneity for ERBB2 amplification [23], which could explain the lack of benefit in clinical trials of other anti-HER2 therapies such as higher doses of trastuzumab [24]. As expected, in our cohort all patients had a signature of chromosomal instability with high frequency of TP53 mutations. A limitation of this study is that enrollment terminated prior completing accrual, however the study reached its primary endpoint as 19 patients were progression-free at 6 months (73%). HER2-overexpressing mGC invariably progresses and develops resistance to trastuzumab. Based on preclinical studies showing that HER2 inhibition can promote T cell activation [25], ongoing studies are investigating the combination of anti-HER2 treatment and immunotherapy in patients with HER2-positive mGC. These include two phase II trials exploring the combination of pembrolizumab with trastuzumab and chemotherapy in HER2-positive mGC (, ). In conclusion, this phase II trial demonstrated the efficacy and safety of the combination of mDCF and trastuzumab in HER2-positive mGC and adds further evidence for this three-drug regimen as a standard option for fit gastric cancer patients.