Angiopoietin-2 induces the neuronal differentiation of mouse embryonic NSCs via phosphatidylinositol 3 kinase-Akt pathway-mediated phosphorylation of mTOR

In this article published in AJTR, we found several images were mixed, resulting in incorrect images mistakenly shown in Figures 3, 4 and 5. We would like to publish this Erratum to reflect this change. The new figures are as follows. The authors apologize for this mistake.

Figure 3.

Activation of mTOR by Ang-2 was mediated by the PI3K/Akt pathway. A. RT-PCR analysis of Tie-2 expression in differentiated NSCs was performed at different time points. B, C. The protein expression levels of p-mTOR and mTOR at different time points over 7 days of Ang-2-mediated neuronal differentiation of NSCs. D, E. The protein expression levels of p-Akt, Akt, p-mTOR and mTOR after treatment with Ang-2 for 7 days. F, G. The dissociated neurospheres were pretreated with LY294002 or rapamycin for 2 hours before Ang-2 treatment. The protein expression levels of p-Akt, Akt, p-mTOR, mTOR, p-p70S6K (Thr⁴²¹/Ser⁴²⁴ and Thr³⁸⁹), and p70S6K were evaluated by Western blot analysis.

Figure 4.

mTOR was involved in the promotion of mouse embryonic NSC neuronal differentiation by Ang-2. A. Dissociated neurospheres were pretreated with rapamycin for 2 hours before Ang-2 treatment and subsequently grown on PLL-coated plates. Representative immunostaining of mouse embryonic NSCs for β-III tubulin. B. The percentage of β-III tubulin-positive neurons was calculated. C. Representative immunostaining of mouse embryonic NSCs for MAP-2. D. The percentage of MAP-2-positive neurons was calculated. Scale bar = 50 μm. *P < 0.05 compared with the control cultures.

Figure 5.

Ang-2-induced neuronal differentiation of mouse embryonic NSCs is dependent on mTOR. A-C. Differentiated mouse embryonic NSCs were treated with Ang-2 and Rapamycin, and the protein expression levels of β-III tubulin and MAP-2 were determined by Western blot. D. Differentiated mouse embryonic NSCs were treated with Ang-2 and Rapamycin, and the protein expression levels of p-Akt, Akt, p-mTOR, mTOR, p-p70S6K (Thr⁴²¹/Ser⁴²⁴ and Thr³⁸⁹), and p70S6K were determined by Western blot. *P < 0.05 compared with the control cultures.