Inhibition and disruption of PAO1 biofilms using Ga treatments. Biofilms were grown on nunc thermanox coverslips for 48 h being treated as described at 37 °C. (A) Biofilm formation of PAO1 with Ga treatments. Formation reduced and prevented increasing Ga concentrations. (ns-No significant difference, * p ≤0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001); (B) Untreated PAO1 with intact biofilm; (C) PAO1 biofilm disrupted and reduced after treatment with 625 µg/mL Ga-NAC particles. Separately conducted CFU analysis indicated 625 µg/mL, which demonstrated a 1 log reduction in the biofilm viability.