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. 2019 Oct 8;10(56):5745–5754. doi: 10.18632/oncotarget.27196

Figure 3. Caspases controls USP5 cleavage.

Figure 3

(A) A375 melanoma cells were treated with 100 ng TRAIL for indicated time in minutes. Cell lysates were subjected to immunoblotting for the protein indicated, including USP5 cleavage. (B) A375 and SK-MEL28 melanoma cells (TRAIL sensitive) were treated first with Caspase-8 inhibitor (50 μM) for hour and then treated with 100ng of TRAIL for 4 hr. Cell lysates were immunoblotted for the protein indicated, including USP5 cleavage. (C) A375 and SK-MEL28 melanoma cells (TRAIL sensitive) were treated first with Caspase- 8 inhibitor (50 uM) for hour and then treated with 100 ng of TRAIL for 4 hr. Cell lysates were blotted for total ubiquitin protein. Actin served as a loading control.