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. 2019 May 31;127(5):059003. doi: 10.1289/EHP5506

Erratum: A Unique Co-Culture Model for Fundamental and Applied Studies of Human Fetoplacental Steroidogenesis and Interference by Environmental Chemicals

Andrée-Anne Hudon Thibeault, Kathy Deroy, Cathy Vaillancourt , J Thomas Sanderson
PMCID: PMC6791573  PMID: 31148504

In Figure 3, the H295R and BeWo cell-type labels are inverted between graphs A and B. This does not alter the conclusion that treatment with prochloraz (1 and 3μM) did not affect the proliferation of either cell type in coculture during the first 24 h. The corrected figure is included in this erratum. The authors regret the error.

Figure 3.

Figures 3A and 3B are line graphs plotting normalized cell index for H295R and B e W o after the effects of dimethyl sulfoxide, Prochloraz (1 micromolar) and Prochloraz (3 micromolars) (ranging from 0.0 to 2.0 at increments of 0.5) and (from 0 to 10 at increments of 2) (y-axis), respectively, across time (ranging from 0 to 96 hours at increments of 24 hours) (x-axis). Figure 3C is a bar graph plotting C Y P 19 acitivity after the effects of dimethyl sulfoxide, Prochloraz (1 micromolar) and Prochloraz (3 micromolars) (ranging from 0 to 200 percent control at increments of 50) across H295R and B e W o cells (x-axis), representing data as standard error of mean. Figures 3D, 3E, and 3F are bar graphs plotting the effects of prochloraz on estradol (ranging from 0 to 100 picograms per milliliter at increments of 25), estriol (ranging from 0 to 500 picograms per milliliter at increments of 100), and estrone (ranging from 0 to 60 picograms per milliliter at increments of 10) across H295R or B e W o cells in monoculture or in co-culture (x-axis).

Effects of prochloraz (1 and 3μM) on the proliferation of H295R (A) and BeWo (B) cells in coculture monitored in real time and its effects on CYP19 activity in each cell line after 24 h of coculture (C). The effects of prochloraz on estradiol (D), estriol (E), and estrone (F) production by H295R and BeWo cells in monoculture or coculture (24-h exposure). Concentrations are presented as mean±SE; n=3. *p<0.05, **p<0.01, and #p<0.001, compared with DMSO control.

Environ Health Perspect 122(4):371 (2014), https://doi.org/10.1289/ehp.1307518


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