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. 2019 Oct 3;8:e48408. doi: 10.7554/eLife.48408

Figure 3. Retrograde tracing with highly-diluted (0.002%) fluorogold prevents perturbation of in vivo electrophysiological properties of identified nigrostriatal dopamine neurons.

(A) Anatomical mapping of all extracellularly recorded and juxtacellularly labelled neurons (projected to bregma −3.16 mm; control in black, FG-labelled in green). Note the anatomical overlap of recorded DA populations in the lSN. Inset, FG-injection site in DLS (FG in blue, TH in green). (B–F) Scatter dot-plots (line at median) showing no significant differences in firing frequency (Hz), coefficient of variation (%), kurtosis and skewness of the ISI-distributions, GLO-based firing pattern (all B), normalized AP waveform, AP duration (ms), relative AP trough (all C), pauses per minute, pause duration (s) (both D), SFB (%), SFB contingency (% of neurons > and < 5% SFB) (both E), bursts per minute, intraburst-frequency (Hz) (both F).

Figure 3—source data 1. Electrophysiological data.
All included electrophysiological parameters analyzed for recorded control and projection-defined DA neurons.
elife-48408-fig3-data1.xls (80KB, xls)
DOI: 10.7554/eLife.48408.007
Figure 3—source data 2. Labelling detection.
Labelling detection parameters (labelling intensity of individual cells, backgrounds, Delta, standard deviation of backgrounds and number of positive FG-positive marks after thresholding) for FG-labelled DA neurons.
elife-48408-fig3-data2.xls (42.5KB, xls)
DOI: 10.7554/eLife.48408.008

Figure 3.

Figure 3—figure supplement 1. Comparison of in vivo firing properties of control and FG-labelled neurons.

Figure 3—figure supplement 1.

(A1–P1) Scatter dot-plots (line at median) and bar graphs showing no significant differences in various parameters of firing. (A2–P2) Feature maps of respective parameters.