Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Col1a1:tetO-H2B-GFP; ROSA:rtTA (H2B-GFP) | Brack, Hochedlinger labs, PMID: 24715455, 19060879 | ||
| Strain, strain background (Mus musculus) | Scx-GFP | Schweitzer lab, PMID: 20059955 | ||
| Strain, strain background (Mus musculus) | Scx-Cre | Schweitzer lab,PMID: 11585810 | ||
| Strain, strain background (Mus musculus) | Gt(ROSA)26Sortm9(CAG.tdTomato)Hze (ai9)/TdTom | Jackson | cat# 007909 | |
| Antibody | Rat anti Mouse CD31 | BD | Cat#551262 | (1:100) |
| Antibody | Rat anti Mouse CD45 | BD | Cat# 557659 | (1:100) |
| Antibody | Mouse monoclonal BrdU | Biolegend | Cat# 339808 | (1:100) |
| Antibody | Rat monoclonal BrdU | Abcam | Cat# 6326 | (1:100) |
| Commercial assay or kit | Green Flow Cytometry Reference Beads | Molecular Probes | Cat# C16508 | |
| Commercial assay or kit | Click-iT EdU | Invitrogen | Cat# C10337 | |
| Commercial assay or kit | Qubit HS RNA assay | Invitrogen | Cat# Q32852 | |
| Commercial assay or kit | SuperScript | Thermo Fisher | Cat#18091050 | |
| Commercial assay or kit | SYBR | Applied Biosystems | Cat#4367659 | |
| Chemical compound, drug | Dox | Sigma | cat#D9891 | |
| Chemical compound, drug | Collagenase II | Worthington | Cat# LS004176 | |
| Chemical compound, drug | DMEM | Gibco | Cat#11956–092 | |
| Chemical compound, drug | P/S | Corning | Cat#30002 CL | |
| Chemical compound, drug | Hepes | Gibco | Cat#15630–80 | |
| Chemical compound, drug | Collagenase I | Gibco | Cat# 17100–017 | |
| Chemical compound, drug | Dispase | Gibco | Cat# 1710541 | |
| Chemical compound, drug | BrdU | Sigma | Cat#B5002 | |
| Chemical compound, drug | Hoechst | Thrmo Fisher | Cat# H3569 | |
| Chemical compound, drug | TRIzol | Invitrogen | Cat# 15596026 | |
| Software, algorithm | Prism8 | GraphPad | ||
| Software, algorithm | R | 'stats' version 3.5.1(Team) |