Figure 4. PRYSPRY variants have profound differences in intrinsic stability.

(A) Differential scanning fluorimetry of PRYSPRY variants to determine their melting temperature (Tm) using changes in intrinsic tryptophan fluorescence that occur upon unfolding (first derivative of 330/350 nm ratio). (B) Differences between variants are plotted as a ΔTm with respective to wild-type. (C) Correlation between ΔΔG for stability of PRYSPRY variants with predicted degree of deleteriousness from various algorithms. Correlation and p-values were calculated using the Pearson correlation coefficient. Data provided in Figure 4—source data 1.