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. 2019 Oct 15;4(5):e00416-19. doi: 10.1128/mSystems.00416-19

FIG 3.

FIG 3

Expression of genes related to AdoMet metabolism and transport. (A) Expression of MetK, cobalamine-dependent methionine synthase (CoDMS), mitochondrial methionine synthase reductase (MMSR), and S-adenosylmethionine hydrolase (SAH) were measured in LiSNFR50.1 and compared to WT cells as determined by qRT-PCR. Expression was quantified relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (B) Expression of AdoMetT1 as measured by qRT-PCR during early (white bar), late logarithmic (gray bar), and stationary (black bar) phases of growth. Expression was quantified relative to GAPDH. (C) Response of Leishmania promastigotes to SNF pressure at early log (●), late log (■), and stationary (▲) phases of growth. All data are means plus SEM for three independent replicates. Statistical analyses were performed using paired (A and B) and unpaired (C) two-tailed t tests. Statistical significance is indicated as follows: **, P < 0.01; *, P < 0.05; ns, not significant.