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. 2007 Jul 4;27(27):7130–7135. doi: 10.1523/JNEUROSCI.1150-07.2007

Acute Stress Facilitates Hippocampal CA1 Metabotropic Glutamate Receptor-Dependent Long-Term Depression

Francis Chaouloff 1,✉, Agnès Hémar 1,*, Olivier Manzoni 2,*
PMCID: PMC6794579  PMID: 17611266

Abstract

Acute stress affects NMDA receptor (NMDAR)-dependent synaptic plasticity in the CA1 region of the hippocampus, with long-term potentiation and long-term depression (LTD) being, respectively, diminished and facilitated by acute exposure to stress. Here, we examined whether this facilitatory effect of stress on NMDAR-dependent LTD extends to metabotropic glutamate receptor (mGluR)-dependent LTD at Schaffer collateral–CA1 synapses. Application of a low dose (50 μm) of the selective group 1 mGluR agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) promoted LTD in slices from stressed, but not from control, rats. Pretreatment of stressed rats with the glucocorticoid receptor (GR) antagonist RU38486 prevented the facilitation of DHPG-induced LTD (DHPG-LTD), indicating the involvement of corticosterone secretion and, in turn, stimulation of GRs. Finally, pretreatment of slices with an mGluR1, but not an mGluR5, antagonist blunted the sensitizing effect of stress on DHPG-LTD. These results indicate that acute stress, through corticosterone stimulation of GRs, facilitates the expression of mGluR1-dependent DHPG-LTD in the hippocampal CA1 region.

Keywords: metabotropic glutamate receptor, long-term depression, acute stress, glucocorticoid receptor, hippocampus, CA1

Introduction

Exposure to acute/chronic uncontrollable stress has major affective (e.g., depression) and cognitive (e.g., impaired learning) consequences (McEwen, 2000; De Kloet et al., 2005). There is overwhelming evidence that numerous stress-elicited changes in behaviors are linked to major modifications in the hippocampal circuitry: according to the duration and intensity of the stressor, these modifications range from functional alterations in synaptic efficacy to gross morphological changes and inhibition of neurogenesis (McEwen, 2000; Kim and Diamond, 2002). In keeping with the tight links established between hippocampal synaptic plasticity, on the one hand, and learning and memory, on the other hand, numerous in vitro and in vivo works have dealt with the consequences of acute stress on NMDA receptor (NMDAR)-dependent long-term potentiation (LTP) and/or long-term depression (LTD). In CA1, acute stress impairs high-frequency stimulation-induced LTP while facilitating low-frequency stimulation (LFS)-elicited LTD (Foy et al., 1987; Shors et al., 1989; Kim et al., 1996; Xu et al., 1997). By means of selective ligands for the two classes of corticosteroid receptors, namely the mineralocorticoid receptor (MR) and the glucocorticoid receptor (GR), it was shown that GRs mediate the aforementioned effects of acute stress on NMDAR-dependent synaptic plasticity in CA1 (Xu et al., 1998; Yang et al., 2004). This conclusion is in keeping with the respective properties of MRs and GRs to be activated by low and high concentrations of corticosterone [such as those reached during stress (De Kloet, 1991)] and the observation in control slices that GR stimulation by exogenous agonists or by high corticosterone concentrations impedes synaptic potentiation while it enhances LTD in CA1 (Diamond et al., 1992; Coussens et al., 1997; Alfarez et al., 2002; Wiegert et al., 2005). Together, these data clearly indicate that stress, through corticosterone release, is endowed with metaplastic properties (Kim and Diamond, 2002).

At hippocampal CA1 synapses, NMDAR-dependent LTD (NMDA-LTD) coexists with another form of LTD mediated by group 1 metabotropic glutamate receptors (mGluRs) (Bolshakov and Siegelbaum, 1994; Oliet et al., 1997). This mGluR-dependent LTD (mGluR-LTD), which can be elicited pharmacologically by the group 1 mGluR agonist (RS)-3,5-dihydroxyphenylglycine (DHPG), is insensitive to NMDAR blockade and does not occlude NMDA-LTD (Palmer et al., 1997; Fitzjohn et al., 1999; Huber et al., 2000, 2001). Moreover, these two forms of LTD differ with respect to their underlying induction/expression mechanisms, including rapid dendritic protein synthesis dependency/independency (Malenka and Bear, 2004). In keeping with the observation that group 1 mGluR ligands are endowed with potent effects in memory and anxiety tests (Spooren et al., 2003), we thus investigated whether acute stress has any influence on DHPG-induced LTD (DHPG-LTD) at CA1 synapses. The results show that acute stress facilitates DHPG-LTD, an effect mediated by corticosterone stimulation of GRs and dependent on mGluR1 but not mGluR5 activation.

Materials and Methods

Animals and stress protocol.

Two-week-old Sprague Dawley rat pups and their mothers (Janvier, Le Genest Saint Isle, France) were kept in temperature- and humidity-controlled quarters under a 12 h light/dark cycle (lights on at 7:00 A.M.). Food and water were provided ad libitum. Rats were weaned 1 week after their arrival, housed collectively by gender, and used between postnatal day 25 (P25) and P42. For stress experiments, rats were restrained for 30 min using individual disposable soft plastic restrainers (Harvard Apparatus, Les Ulis, France), as described previously (Sarrieau et al., 1998), and were allowed a 90 min period of recovery before being deeply anesthetized with isoflurane and decapitated. All experiments were conducted in strict compliance with European directives and French laws on animal experimentation (authorization number 06369).

Hippocampal slice preparation and electrophysiology.

Brains from control and stressed rats were sliced (400 μm) in the horizontal plane using a Vibratome (Integraslice; Campden Instruments, Leicester, UK). Slices were collected in an ice-cold dissection buffer containing (in mm) 126 NaCl, 18 NaHCO3, 2.5 KCl, 2.4 MgCl2, 1.2 CaCl2, 1.2 NaH2PO4, and 11 glucose, under constant saturation with 95% O2/5% CO2. All slices, bearing a cut made between areas CA3 and CA1 to prevent epileptic activity, were then transferred for 1–4 h in a storage chamber filled with a temperature-controlled (30°C) artificial CSF (ACSF) containing (in mm) 126 NaCl, 18 NaHCO3, 2.5 KCl, 1.2 MgCl2, 2.4 CaCl2, 1.2 NaH2PO4, and 11 glucose, under constant saturation with 95% O2/5% CO2. Slices were then placed in a submersion-type chamber continually perfused (2 ml/min) with a 30°C oxygenated ACSF containing 100 μm picrotoxin. Schaffer collateral afferents in stratum radiatum were stimulated (0.033 Hz, 100 μs duration) using bipolar tungsten electrodes. The stimulation intensity (0.20–0.25 mA) was chosen to evoke 40–60% of the maximal responses. The recording pipette was filled with ACSF and placed in CA1 stratum radiatum. Both the field EPSP (fEPSP) slope and amplitude were measured (graphs depict amplitude). An Axopatch-1D (Molecular Devices, Foster City, CA) was used to record the data, which were filtered at 1–2 kHz, digitized at 5 kHz on a DigiData 1200 interface (Molecular Devices), and collected on a personal computer using Clampex 9.2 (Molecular Devices). These data were then analyzed using Clampfit 9.2 (Molecular Devices). In all experiments, DHPG (50 or 100 μm) was applied for 10 min, and its effects on fEPSPs were monitored up to 50 min after the onset of that application (Rouach and Nicoll, 2003). In the text, calculations of maximal short-term depressive effects of DHPG refer to the 5 min period showing the highest inhibition of fEPSP responses, whereas calculations on long-term effects of DHPG refer to the last 5 min of the washout period. In experiments aimed at measuring the impacts of mGluR antagonists, these were present throughout the entire recording procedure. For the estimation of the paired-pulse ratio (PPR), pairs of stimuli (50 ms interval) were delivered every 30 s. The PPR was calculated as the ratio of the amplitude of the fEPSP evoked by the second stimulus on the amplitude of the fEPSP evoked by the first stimulus. To assess the immediate and long-term effects of DHPG on PPRs, these PPRs were measured during the last 5 min of DHPG application and the last 5 min of the washout period, respectively. Cumulative probability distributions were based on the last 5 min recordings.

Drugs.

2-Amino-5-phosphonopentanoic acid (AP-5), (RS)-DHPG, 2-methyl-6-(phenylethynyl)-pyridine (MPEP), and 7-(hydroxyimino)cyclopropa(β)chromen-1a-carboxylate ethyl ester (CPCCOEt) were from Tocris-Fischer Bioblock (Illkirch, France). Picrotoxin, RU38486 (RU486), and Tween 20 were from Sigma-Aldrich (Saint Quentin Fallavier, France). AP-5 (100 mm) was stocked in 0.1 m NaOH, DHPG (20 mm) and MPEP (10 mm) were stocked in water, and picrotoxin (100 mm) and CPCCOEt (50 mm) were stocked in DMSO (all stocks kept frozen). Note that DHPG stocks were freshly prepared every week. RU486 (40 mg/kg) was dissolved in water containing a droplet of Tween 20 and injected subcutaneously (2 ml/kg) in the flanks of control and stressed rats 150 min before they were killed (i.e., 30 min before stress). The vehicle solution (water and Tween 20) was administered according to a similar paradigm.

Statistics.

All data, presented as mean ± SEM, were compared by means of Student's t tests (two-group comparisons) and ANOVAs with/without repeated measures, followed, if significant, by Tukey's multiple comparison tests. In all tests, the significance level was preset to p < 0.05.

Results

Acute stress facilitates DHPG-LTD

Input–output curves of fEPSP amplitude responses to gradual increases in stimulation intensity were similar in slices from control and stressed animals (Fig. 1A), suggesting that stress did not affect basal synaptic transmission. A 10 min application of 50 μm DHPG in control slices promoted an initial depression in excitatory synaptic transmission that returned toward baseline levels by the first 20 min of the washout period (Fig. 1B,C). In marked contrast, in slices from stressed animals, the initial depression was followed by LTD (last 5 min of washout: 18 ± 4%, p < 0.05 compared with control slices) (Fig. 1B,C). Indeed, cumulated data from the entire study showed that 41 ± 2% of control slices and 84 ± 2% of slices from stressed rats (p < 0.001) displayed LTD during the last 5 min of recordings (supplemental Fig. S1A,B, available at www.jneurosci.org as supplemental material) and that the facilitatory effect of stress on DHPG-LTD was independent from the animal gender (supplemental Fig. S1C–F, available at www.jneurosci.org as supplemental material). The inability of 50 μm DHPG to elicit LTD in control slices was accounted for by the short latency between slice preparation and recordings (1–4 h; see above) because control slices given 4–7 h of rest displayed LTD after DHPG application (supplemental Fig. S2, available at www.jneurosci.org as supplemental material).

Figure 1.

Figure 1.

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Acute stress facilitates mGluR-LTD in the rat hippocampal CA1 region. A, fEPSP responses to increasing stimulus intensities do not differ between slices from control and stressed rats. B, A 10 min application of 50 μm DHPG elicits LTD in slices from stressed but not from control rats. Whether analyzed during the last 10 or 20 min of recordings, stress, but not the stress × time interaction, proves significant (F(1,162) = 7.64, p = 0.013 and F(1,342) = 9.05, p = 0.008, respectively). Inset, Representative fEPSPs taken at the time indicated on the graph below. Calibration: 0.5 mV, 10 ms. C, Cumulative probability distribution of the normalized fEPSPs represented as averages in B. In this and subsequent figures, each point is the average of two successive responses, and dashed lines represent 100% of baseline. Data are represented as mean ± SEM.

As opposed to the application of 50 μm DHPG, 100 μm DHPG promoted LTD in control slices (including in the presence of 100 μm of the NMDAR antagonist AP-5) (supplemental Fig. S3A, available at www.jneurosci.org as supplemental material), the amplitude of which was similar to that measured in slices from stressed rats (15 ± 4 and 26 ± 5%, respectively) (supplemental Fig. S3B, available at www.jneurosci.org as supplemental material). These results indicate that the stress paradigm used herein solely potentiates the effects of a subthreshold activation of mGluRs to induce LTD.

We next analyzed PPRs after 50 μm DHPG application to slices from control and stressed animals. The respective baseline PPRs did not differ between the two groups of slices (1.28 ± 0.09 and 1.30 ± 0.06 for control and stress, respectively; n = 8 in each group). Besides, although DHPG application affected PPRs in control slices (F(2,21) = 5.58; p < 0.05) and in slices from stressed rats (F(2,21) = 5.18; p < 0.05), post hoc comparisons revealed that the PPR increased during but not after that application in both slice groups (Fig. 2). This suggests that the facilitation of DHPG-LTD is not associated with changes in presynaptic release probability.

Figure 2.

Figure 2.

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The facilitatory effect of acute stress on DHPG-LTD is not associated with a change in the PPR. A, Individual PPR absolute values before (baseline), during (DHPG 50 μm), and after (wash) a 10 min application of DHPG to slices from control and stressed rats (n = 8 per group). Inset, Representative fEPSPs taken at the time indicated on the graph below. Calibration: 0.5 mV, 10 ms. B, Percentage of PPR changes from baseline during (DHPG 50 μm) and after (wash) a 10 min application of DHPG to slices from control and stressed rats. The PPR increases during (*p < 0.05) but not after DHPG application in both slice groups. Data are represented as mean ± SEM.

The facilitatory effect of stress on DHPG-LTD is mediated by GRs

Because the restraint paradigm used herein promotes sustained corticosterone release (Sarrieau et al., 1998), we investigated whether GRs are involved in the LTD elicited by 50 μm DHPG. To this aim, control and stressed rats were pretreated (150 min before they were killed, i.e., 30 min before stress) with the GR antagonist RU486 (40 mg/kg) or its vehicle. Analyses of the long-term effects of 50 μm DHPG application to slices from vehicle-injected animals (Fig. 3A,B) confirmed that stress facilitates LTD (18 ± 5%; p < 0.01 for the difference with controls). Besides, RU486 pretreatment selectively abolished the impact of stress on DHPG-LTD (Fig. 3C,D). Of note is the observation that in slices from stressed rats, RU486 pretreatment did not affect the maximal amplitude of the short-term inhibition triggered by DHPG (82 ± 4 and 71 ± 4% of baseline levels in vehicle- and RU486-preteated groups, respectively) (Fig. 3C), indicating a selective involvement of GRs on LTD expression.

Figure 3.

Figure 3.

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The facilitatory effect of acute stress on DHPG-LTD is mediated by GRs. A, A 10 min application of 50 μm DHPG elicits LTD in slices from stressed but not from control rats pretreated with vehicle (Veh). Whether analyzed during the last 10 or 20 min of recordings, stress, but not the stress × time interaction, proves significant (F(1,126) = 11.72, p = 0.004 and F(1,266) = 10.09, p = 0.007, respectively). B, Cumulative probability distribution of the normalized fEPSPs represented as averages in A. C, A 10 min application of 50 μm DHPG fails to induce LTD in slices from stressed rats pretreated (30 min before stress) with RU486. D, Cumulative probability distribution of the normalized fEPSPs represented as averages in C. Data are represented as mean ± SEM.

Together, these results indicate that the facilitatory effect of stress on DHPG-LTD requires corticosterone release and, in turn, GR stimulation.

The facilitatory effect of stress on DHPG-LTD involves mGluR1

We next evaluated the respective influences of the selective mGluR1 antagonist CPCCOEt (50 μm) (Litschig et al., 1999) and the selective mGluR5 antagonist MPEP (10 μm) (Gasparini et al., 1999) on fEPSP responses to DHPG application in slices from control and stressed animals. In control slices, the maximal amplitude of the DHPG initial inhibitory effect varied with the treatments (F(2,17) = 4.43; p < 0.05). Thus, such maximal amplitude was decreased after either CPCCOEt (50 ± 3% of baseline levels; p < 0.05) or MPEP (45 ± 7% of baseline levels; p < 0.05) compared with vehicle-treated slices (71 ± 7% of baseline levels) (Fig. 4A). In slices from stressed rats, treatments affected the extent to which DHPG exerted its maximal short-term effect (F(2,23) = 7.90; p < 0.01) with CPCCOEt (38 ± 9% of baseline levels; p < 0.01) but not MPEP (65 ± 5% of baseline levels), reducing that response compared with vehicle-treated slices (71 ± 5% of baseline levels) (Fig. 4C). Long-term effects of DHPG, absent in control slices (Fig. 4A,B), varied with the treatments in slices from stressed rats (F(2,23) = 3.65; p < 0.05), with CPCCOEt (1 ± 3%; p < 0.05), but not MPEP (14 ± 3%), preventing DHPG-LTD (22 ± 6% in vehicle-treated slices) (Fig. 4C,D).

Figure 4.

Figure 4.

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DHPG-LTD in slices from stressed rats is mediated by mGluR1. A, The short-term inhibitory effect of a 10 min application of 50 μm DHPG in control slices is weakened either by 50 μm of the mGluR1 antagonist CPCCOEt or by 10 μm of the mGluR5 antagonist MPEP. B, Cumulative probability distribution of the normalized fEPSPs represented as averages in A. C, Treatment with 50 μm of the mGluR1 antagonist CPCCOEt weakens the short-term inhibitory effect of a 10 min application of 50 μm DHPG and blocks DHPG-LTD in slices from stressed rats. Whether analyzed during the last 10 or 20 min of recordings, stress, but not the stress × time interaction, proves significant in vehicle-pretreated (F(1,162) = 6.18, p = 0.022 and F(1,342) = 4.84, p = 0.04, respectively) and MPEP-pretreated (F(1,99) = 10.02, p = 0.01 and F(1,209) = 8.96, p = 0.013, respectively) slices but not in CPCCOEt-pretreated slices. D, Cumulative probability distribution of the normalized fEPSPs represented as averages in C. For clarity, MPEP is not presented in B and D. Veh, Vehicle. Data are represented as mean ± SEM.

Thus, these experiments demonstrate that mGluR1, but not mGluR5, mediates DHPG-LTD in slices from stressed rats.

Discussion

The present results show that stress, through corticosterone stimulation of GRs, lowers the threshold for mGluR1-dependent DHPG-LTD in the hippocampal CA1 region.

So far, the impact of acute stressors and/or manipulations of the hypothalamo–pituitary–adrenal axis on hippocampal CA1 homosynaptic plasticity have focused on NMDAR-dependent LTP and LTD. In vitro and in vivo findings indicate that LFS (1–3 Hz, 15 min) triggers LTD in stressed animals while proving ineffective in the long term in control animals (Kim et al., 1996; Xu et al., 1997; Yang et al., 2004). Because acute stress also impairs synaptic potentiation (Foy et al., 1987; Shors et al., 1989; Xu et al., 1997; Alfarez et al., 2002), it has been proposed that stress has metaplastic properties (Kim and Diamond, 2002). Metaplasticity refers to the resetting of the respective thresholds for LTP and/or LTD without any permanent change in synaptic efficacy (Abraham and Bear, 1996). In this context, our observation that an ineffective concentration of DHPG in control slices proved effective in promoting LTD in slices from stressed animals strongly suggests that stress metaplastic effects in the hippocampal CA1 region extend to mGluR-LTD. When the DHPG concentration was effective in triggering LTD in control slices, stress did not further enhance LTD. This indicates that stress targets the mechanisms allowing the expression of DHPG-LTD, the efficiencies of which are yet set to their maximal level by stress.

Studies of PPR changes have suggested that, depending on the developmental stage, presynaptic (<P15) or postsynaptic (>P21) mechanisms underlie the expression of DHPG-LTD in rats (Huber et al., 2000; Nosyreva and Huber, 2005; Zhang et al., 2006) (but see Fitzjohn et al., 2001; Moult et al., 2006). In our preparation, DHPG application to slices from stressed adolescent (>P21) rats elicited a rapid increase in PPR but was without effect on long-term PPR. This result thus suggests that the facilitatory influence of stress on the expression of DHPG-LTD involves postsynaptic mechanisms.

Circulating corticosterone concentrations and LTP amplitude in hippocampal CA1 follow an inverted U-shape relationship (Diamond et al., 1992). Conformingly, pretreatment of stressed rats with a GR blocker prevents both the inhibitory influence of stress on NMDA-LTP and stress-elicited facilitation of NMDA-LTD (Xu et al., 1998; Yang et al., 2004). This GR-mediated facilitatory influence of stress on NMDA-LTD extends to DHPG-LTD because pretreatment with a GR antagonist prevented the long-term effects of DHPG in slices from stressed rats. The lack of effect of GR blockade in controls clearly indicates that restraint-elicited corticosterone release is responsible for the facilitation of DHPG-LTD.

The quest for the identity of the mGluR subtype involved in the expression of DHPG-LTD has provided contradictory results (Gasparini et al., 1999; Huber et al., 2001; Faas et al., 2002; Hou and Klaan, 2004; Nosyreva and Huber, 2005). Beside discrepancies likely attributable to differences in animal species/age and DHPG concentrations, a recent electrophysiological and biochemical study has shown that the duration of exposure to the antagonists and to DHPG is an important variable (Volk et al., 2006). Taking into account that variable, it was reported that mGluR1 or mGluR5 mediates the induction of DHPG-LTD, whereas mGluR1 is fully responsible for the expression of that LTD (Volk et al., 2006). Using a similar pharmacological approach (i.e., continuous presence of the antagonists throughout the experiment), we found that mGluR1 blockade diminished the amplitude of the DHPG acute inhibitory effect and prevented the facilitatory influence of stress on DHPG-LTD. Because mGluR1 or mGluR5 blockade diminished the acute inhibitory influence of DHPG in controls, our results indicate that stress, through corticosterone release and GR stimulation, favors a selective stimulation of mGluR1 at the expense of mGluR5. The observation that mGluR1 as opposed to mGluR5 stimulation does not potentiate NMDA currents in CA1 (Mannaioni et al., 2001) in turn suggests that the aforementioned effects of stress are independent from NMDARs.

Repeated/chronic stress is associated with a facilitation of LFS-elicited LTD in hippocampal CA1, a change prevented by chronic antidepressant treatment (Von Frijtag et al., 2001). Moreover, using a different stimulus protocol (paired-pulse LFS) in adult animals, chronic stress was found to facilitate LTD in hippocampal CA1 (Holderbach et al., 2007). Again, such facilitation vanished if animals were treated with a chronic antidepressant regimen (Holderbach et al., 2007). These results suggest that plasticity changes occurring with repeated/chronic exposure to stress may reflect a lack of adaptation to the stressor. Bearing in mind the results from the present study as well as the anxiolytic and/or antidepressant profiles of several type 1 mGluR ligands (Spooren et al., 2003), future experiments should test whether CA1 DHPG-LTD is sensitive to chronic stress as do other forms of plasticity (see above) and, if so, whether such a sensitivity is adaptive or not.

Footnotes

This work was supported by grants from the Centre National de la Recherche Scientifique (CNRS), Inserm, and Région Aquitaine. We thank Dr. F. De Bock for help with the slice preparation, Dr. J. P. Pin for advice regarding mGluR pharmacology (both at CNRS and Inserm, Montpellier, France), and Drs. C. Mulle and L. Groc (CNRS, Bordeaux, France) for critical reading of this manuscript.

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