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. 2019 Nov;25(11):1509–1521. doi: 10.1261/rna.072256.119

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© 2019 Martelly et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — GST-UBL specifically binds to the U1 snRNP via U1-SL4. (A) Northern analysis for spliceosomal snRNAs in GST-UBL pull-down complexes. GST-UBL/WT protein was incubated in HeLa cell nuclear extracts in the absence or presence of U1-SL4/WT or M10 RNAs as competitors, followed by pull-down using glutathione agarose. (B) Western blot analysis of proteins in the pull-down complexes using antibodies to U1C, U1-70k, and SF3A3 proteins. (C) Western analysis of proteins present in GST-UBL/WT, Y772C, Y773 pull-down complexes. I and AP indicate input and affinity pull-down complexes, respectively.