Figure 7.

Inducible Cre-recombinase strategy for labeling P25-born OSNs in the OE following basal cell division. A, Ascl1^CreERT2/R26R^ZsGreen double transgenic mice were obtained by crossing an Ascl1^CreERT2/+ transgenic mice line with an R26R^{ZsGreen/ZsGreen} transgenic mice line. 4OH-Tx induces Cre recombination in Ascl1⁺ cells, which results in the expression of ZsGreen in Ascl1^CreERT2/R26R^ZsGreen double transgenic mice. B–D, Cre-recombinase strategy controls: Ascl1^CreERT2/R26R^ZsGreen double transgenic mice show olfactory sensory neuron-specific labeling 12 d after 4OH-Tx injection (B), but no specific labeling after sunflower oil injection, although some nonspecific labeling is present deep in the OE and beneath the basal lamina (C); Ascl1⁺/R26R^ZsGreen transgenic mice show no labeling after 4OH-Tx injection (D). Scale bars, 300 μm.