Fig. 11.

HFD/PS mice display impaired hippocampal neurogenesis, reduced number of parvalbumin− + interneurons,and halved BDNF levels. (a) Representative images of BrdU-positive cells (red) in the DG per each group. Green, NeuN counterstaining. (b) Representative images of detection of parvalbumin (PV)-positive cells resident in the DG per each experimental group. (c) Number (#) of BrdU-positive cells per DG. (d) Number (#) of PV-positive cells per DG. (e) Hippocampal brain-derived neurotrophic factor (BDNF) protein levels. (f) Hippocampal TrkB protein levels. (g) Representative images of cropped densitometric bands of hippocampal TrkB expression in Control and Ob + PS mice. The full-length blots/gels are presented in Supplementary Fig. 5. All values are represented trough box-and-whisker plots (n = 5, two-way ANOVA, *p < .05, **p < .01, ***p < .001 Vs Controls; ^#p < .05 Vs Ob; ^$$p < 0·01, Vs PS). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)