Fig. 1.

SOX2 is expressed in a subset of EwS patients and correlates with event-free survival (EFS) and overall survival (OS).

(A). Microarray analysis of SOX2 mRNA expression in 189 primary EwS cases.

(B). Kaplan-Meier analysis of OS in 189 EwS patients stratified by their SOX2 mRNA expression level (cut-off 80th percentile).

(C). Analysis of SOX2 protein expression levels in an independent cohort of 141 EwS patients in a TMA by IHC.

(D). Representative images for SOX2 IHC (nuclear staining) in EwS patients with different percentages of nuclear SOX2 immunoreactivity. Scale bars = 50 μM (40×) and 200 μM (10×).

(E). Kaplan-Meier analysis of EFS in 141 EwS patients stratified by their SOX2 protein expression level (cut-off >10% positive nuclei).

(F). Kaplan-Meier analysis of OS in 141 EwS patients stratified by their SOX2 protein expression level (cut-off >10% positive nuclei).