Fig. 1.

Log₂-fold changes of primary metabolites in the flag leaves of the cold-sensitive Wyalkatchem (W) and cold-tolerant Young (Y) after one night (TP₁ vs. TP₀) and prolonged (TP₃ vs. TP₁) of cold treatment. Fold changes were calculated by dividing the concentration of the variety at a time point (e.g., TP₁) to the concentration of that variety at the previous time point (e.g., TP₀), then Log₂-transformed. Significance of difference was determined by Benjamini and Hochberg method (Benjamini and Hochberg 1995) with false discovery rate (FDR)-adjusted p-value of 0.05 as the cut-off. Green significant decrease, Red significant increase. There were four biological replicates (n = 4) for all the measured metabolites, except phytohormones (n = 3)