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. 2019 Sep 26;24(19):3499. doi: 10.3390/molecules24193499

Figure 1.

Figure 1

Synthesis of RXP470-modified nanostructured lipid carriers (NLC) and control NLC. (a) Addition of dithiothreitol (DTT), incubation 2 h at room temperature, then dialysis overnight. (b) Addition of RXP470.1-maleimide, 4 h incubation at room temperature, then overnight at 4 °C. (c,d) Addition of 1-(2-hydroxyethyl)-1H-pyrrole-2,5-dione, 30 min incubation at room temperature, then dialysis for 24 h.