Table 2.
Selected comparative studies for EV isolation.
| Isolation Methods | Characterization Techniques | Samples | Study |
|---|---|---|---|
| dUC, SEC | NTA, Dissociation-enhanced lanthanide fluorescence immunoassay, WB, TEM | rat plasma, cell culture | [87] |
| dUC, SEC | TEM, AFM, WB, MS | cell culture | [88] |
| Affinity-based (exoEasy kit) and SEC (qEV) | WB, TEM, NTA, lipid quantification kit, RNA quality | plasma | [89] |
| dUC and Commercial kit from Invitrogen, 101Bio, Wako and iZON | Dynamic Light Scattering, immunoblot analysis, qRT-PCR, MS, Cell Proliferation Assay | cell culture | [90] |
| dUC, precipitation (ExoQuick, Total Exosome Isolation Reafent, Exo-PREP) and SEC (qEV) | TEM, NTA, WB | cell culture | [91] |
| Lectin-based, Exoquick, Total exosome Isolation and in-house modified procedure | WB, Reverse transcriptase and qPCR, EM | urine | [92] |
| dUC, precipitation (ExoQuick, Total exosome isolation, PEG, Exo-spin), filtration (ExoMir) | NTA, Flow cytometry, WB, PCR, | serum | [93] |
| dUC, filtration (Stirred cell and Centricon), OptiPrep DG, ExoQuick, Exo-spin, SEC | Tunable resistive pulse sensing, EM, WB | cell culture and plasma | [55] |
| SEC and Exo-Spin | NTA, Flow cytometry, MS | plasma | [62] |
| dUC, anti-EpCAM, OptiPrep DG | MS, WB, TEM | plasma | [79] |
| Nanomembrane ultrafiltration, dUC and dUC-SEC | MS, TEM, WB | urine | [94] |
| dUC, anti-EpCAM, OptiPrep DG | TEM, CryoEM, MS | cell culture | [50] |
| Sucrose DG and ExoQuick | TEM, NTA, WB | serum | [95] |
* EM: electron microscopy; TEM: transmission electron microscopy; NTA: nanoparticle tracking analysis AFM: atomic force microscopy; WB: western blot.