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Journal of Bacteriology logoLink to Journal of Bacteriology
. 2019 Oct 21;201(22):e00777-18. doi: 10.1128/JB.00777-18

Characterization and Comparative Analysis of the Staphylococcus aureus Genomic Island vSaβ: an In Silico Approach

Anita J Kläui a, Renate Boss b, Hans U Graber a,✉
Editor: Michael J Federlec
PMCID: PMC6805111  PMID: 31451542

With the rapid increase of available sequencing data on clinically relevant bacterial species such as S. aureus, the genomic basis of clinical phenotypes can be investigated in much more detail, allowing a much deeper understanding of the mechanisms involved in disease. We characterized in detail the S. aureus genomic island vSaβ and defined a superordinate system to categorize S. aureus strains based on their vSaβ type, providing information about the strains’ virulence-associated genes and clinical potential.

Keywords: Staphylococcus aureus, genomic islands, in silico characterization, comparative analysis

ABSTRACT

Staphylococcus aureus is a widespread opportunistic pathogen to humans and animals. Of its genome, 20 to 25% varies between strains and consists of phages, pathogenicity islands, transposons, and genomic islands. S. aureus harbors up to three genomic islands, vSaα, vSaβ, and vSaγ. The vSaβ region of S. aureus can encode a number of virulence-associated factors, such as serine proteases, leukocidins, enterotoxins, bacteriocins, or a hyaluronate lyase. In this study, the vSaβ regions of 103 clinically relevant S. aureus strains were characterized in silico and compared to the three predefined vSaβ types. We here suggest a superordinate system of 15 different vSaβ types, of which 12 were newly defined. Each vSaβ type has a distinct structure with a distinct set of genes, which are both highly conserved. Between the different types, gene content and composition vary substantially. Based on our data, a strain’s vSaβ type is strongly coupled with its clonal complex, suggesting that vSaβ was acquired in an ancestral S. aureus strain, arguably by phage mediation, before differentiation into clonal complexes. In addition, we addressed the issue of ambiguous nomenclature in the serine protease gene cluster and propose a novel, phylogeny-based nomenclature of the cluster contained in the vSaβ region.

IMPORTANCE With the rapid increase of available sequencing data on clinically relevant bacterial species such as S. aureus, the genomic basis of clinical phenotypes can be investigated in much more detail, allowing a much deeper understanding of the mechanisms involved in disease. We characterized in detail the S. aureus genomic island vSaβ and defined a superordinate system to categorize S. aureus strains based on their vSaβ type, providing information about the strains’ virulence-associated genes and clinical potential.

INTRODUCTION

Staphylococcus aureus is a commensal colonizer of the skin and mucous tissue of up to two-thirds of the human population (1, 2). It is an opportunistic pathogen in humans and animal hosts (3, 4), and its pathogenic potential ranges from being relatively harmless to causing potentially deadly infections (5). The S. aureus genome consists of the core genome and the accessory (or auxiliary) genome. The core genome, representing about 75%, is conserved in all S. aureus strains and consists largely of genes related to metabolism or other housekeeping functions. The accessory genome, about 20 to 25%, consists of mobile (or formerly mobile) genetic elements (MGE), such as bacteriophages, chromosomal cassettes, pathogenicity islands, transposons, and genomic islands (6). Its genes differ between strains (7–9) and are often related to virulence and antibiotic resistance. Therefore, they have important clinical implications, especially when considering their potential to spread horizontally (6, 7). The combination of these variable regions is the key to the high phenotypic variability in S. aureus.

S. aureus strains carry three different classes of genomic islands, vSaα, vSaβ, and the substantially smaller vSaγ. These genomic islands are extremely stable and have highly conserved genes. However, the gene compositions of each of the islands can vary substantially between some strains and are yet identical between others (8, 10).

In 2008, when Baba et al. (8) classified 12 sequenced S. aureus strains into 4 vSaα types and 3 vSaβ types, the comparison of the genomic islands was rather limited. To date, over 10,000 S. aureus whole genomes (including contigs and scaffolds) are available in the NCBI database. Such an enormous increase in sequencing data calls for a new, superordinate system to classify S. aureus strains based on genomic islands. The genomic island vSaβ is of particular interest, as it carries two genes belonging to the type I staphylococcal restriction-modification (RM) system (hsdM and hsdS) and harbors a number of virulence-associated genes, such as a hyaluronate lyase precursor gene (hysA), a lantibiotic gene cluster (bacteriocins of S. aureus [bsa]), two leukocidin genes (lukD and lukE), an enterotoxin gene cluster (EGC), and a cluster of serine protease genes (serine protease like [spl] genes).

First characterized in 2001, the spl cluster was described as an operon containing 6 genes (splA, splB, splC, splD, splE, and splF) with DNA sequence similarities ranging from 42% to 94% (11). For listing new spl genes, the existing alphabetical nomenclature is not ideal. Therefore, we establish an approach to unambiguously name the genes in the spl cluster, including new ones, based on their phylogenetic relationships.

To our knowledge, the findings of Baba et al. (8) have not been proceeded. Hence, we extended their vSaβ listing to a total number of 15 types by adding 12 new types obtained by analysis of the vSaβ islands of 103 clinical S. aureus strains. Thus, striking conservation of the virulence-associated genes was found within each type.

RESULTS

Of the 103 analyzed strains, 37 strains showed known vSaβ types (I to III), and 66 strains showed novel vSaβ types (IV to XV) (see Table 1; also see File S2 in the supplemental material).

TABLE 1.

Amino acid sequence identity of vSaβ-encoded proteinsa

vSaβ type Strain Amino acid identity (%) for protein:
Sav1803 HysA HsdS HsdM SplD2 (2) SplD1 SplD2 SplD3 SplD4 SplC SplB SplA Bla BsaG BsaE BsaF BsaP BsaD BsaC BsaB BasA2 BasA1 LukD LukE Seg Sen Seu ϕent2 ϕent1 Sei Sem Seo tRNA cluster Sav1831
I Mu50 (ref.) + + + + + + + + + * + + + + + + + + + + +
N315 100 99 100 100 100 100 100 100 100 100* 100 100 100 100 100 100 100 100 100 100 100
502A 100 99 100 100 100 100 100 100 100 100* 100 100 100 100 100 100 100 100 100 100 100
HOU1444-VR 100 99 100 99 100 99 100 100 100 100* 100 100 100 100 100 100 100 100 100 100 100
JH1 100 99 100 97 100 100 100 100 100* 100 99 100 100 100 100 100 100 100 100 100
JH9 100 99 100 97 100 100 100 100 100* 100 99 100 100 100 100 100 100 100 100 100
Lodi13K 100 87 84 83 100 100 100 100 99* 100 100 100 100 100 100 100 99 100 100 100
Mu3 100 100 100 100 100 100 100 100 100 100* 100 100 100 100 100 100 100 100 100 100 100
NZAK3 100 99 100 100 100 100 100 100 100 100* 100 100 100 100 100 100 100 100 100 100 100
UCI28 100 99 100 100 22* 100 100 100 100 100* 100 100 100 100 100 100 100 100 100 100 100
ST228 100 99 100 100 46* 99 100 99 100 100* 100 100 100 100 100 100 100 100 100 100 100
II TW20 (ref.) + + + + + + + + + + + + + + + + 58* + + + + + +
COL 100 100 100 99 100 100 100 100 98 100 100 100 100 100 100 99 99 100 70* 100 99 100 100
DSM 20231T 100 99 99 100 100 100 99 100 99 100 100 100 100 100 100 100 R 100 100 100 13* 100 99
G12B 100 100 94 99 100 100 99 100 100 100 100 77* 100 100 100 100 100 100 100 100 99 100 100
G29N 100 100 99 99 100 100 99 100 100 100 100 100 100 100 100 100 100 100 97 100 99 100 100
Lodi10B 100 100 51* 99 100 100 99 100 100 100 100 77* 100 99 100 100 100 100 100 100 99 100 100
M2084B 100 100 5* 99 100 100 99 100 100 100 100 77* 100 100 100 100 100 100 100 100 99 100 100
M2130B 100 100 8* 99 100 100 99 100 100 100 100 77* 100 100 100 100 100 100 100 100 99 100 100
M2529B 100 100 8* 99 100 100 99 100 100 100 100 77* 100 100 100 100 100 100 100 100 99 100 100
M5512B 100 100 99 99 100 100 99 100 100 100 100 77* 100 100 100 100 100 100 100 100 99 100 100
M5171B 100 100 100 99 100 100 99 100 100 100 100 77* 100 100 100 100 100 100 100 100 13* 100 100
M6020B 100 16* 19* 99 100 100 99 100 100 100 100 77* 100 100 100 100 100 100 99 100 99 100 100
MSSA476 90 99 99 99 98 99 99 99 100 77* 100 100 100 100 100 100 100 100 99 99 100
MW2 90 99 99 99 99 99 99 100 100 100 100 100 100 100 99 100 100 100 99 99 100
NCTC8325 100 99 99 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 13* 100 99
Newman 100 100 100 100 100 100 100 100 98 99 100 100 100 100 100 100 100 100 100 100 99 100 100
USA300 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 99 99 100
III MRSA252 (ref.) + + + + + * * + + * + + + + + + + +
55/2053 100 99 100 73* 100 R R 100 100 100* 100 100 100 100 99 100 100 100
A017934/97 100 100 100 100 100 47* 100 100 100 100* 100 100 100 100 100 100 100 100
CIG1605 100 100 100 100 100 47* 17* 100 100 100* 100 100 100 100 100 100 100 100
ATCC 25923 99 24* 100 100 100 100 100 100 99 100* 100 100 100 100 100 100 100 100
ST1632 100 100 100 100 100 47* 17* 100 100 100* 100 100 100 100 100 100 100 100
ST2788 100 100 100 100 100 47* 17* 100 100 100 100 100 100 100 100 100 100 100
HAR21 100 100 100 100 100 47* 17* 100 100 100* 100 100 100 100 100 99 100 100
TCH60 100 99 100 40* 99 100 99 99 100 100* 100 100 100 100 100 100 100 100
IV M3783C (ref.) + + * * + + + + * + + + + + + + + + + + + * + + + * + + +
RF122 100 100 100* 100* 100 100 100 100 99* 99 100 100 100 100 100 100 99 100 100 100 99 100* 100 100 99 100* 100 100 100
M2839C 100 100 100 100 100 100 100* 99 100 100 100 99 100 100 100 100 100 100 100 100* 87 100 100 100* 100 100 100
M1280C 100 100 100* 100 100 100 100 100* 99 99 100 100 100 100 100 99 100 100 100 100 100* 100 100 100 100* 100 99 100
M1655C 100 100 100* 99 100 100 100 100* 99 100 100 100 100 100 100 100 100 100 100 100 100* 100 100 100 100* 100 99 100
M2323C 100 100 100* 100 100 100 100 100* 99 100 100 100 100 100 100 99 100 100 100 100 100* 100 100 100 100* 100 100 100
M2682A 100 100 100* 100* 100 100 100 100 100* 99 100 100 100 95 100 100 100 100 100 100 100 100* 100 100 100 100* 100 80 65
Sa110 100 100 15* 100 100 100 100 99* 99 100 100 100 100 100 100 100 100 100 99 99 100* 100 100 99 100* 100
V S0385 (ref.) + + + +
GD705 100 100 100 100
GD1677 100 99 100 100
NZ15 100 100 100 29
RVIM 100 100 100 100
UB08 100 100 100 100
USA7 100 100 100 100
VET030 100 100 100 100
08BA 100 100 100 100
VI K2R (ref.) + + + + + + + + + + + * + + + +
STDY-6124959 98 100 98 99 100 100 100 100 100 100 100 100* 100 100 100 100
HST-066 98 100 98 99 100 100 100 100 100 100 100 100* 100 100 100 100
M0443 98 100 98 99 100 100 100 100 100 100 100 100* 100 14* 100 14
1608 S21 100 100 100 100 100 100 100 99 100 100 100 100* 100 100 100 100
st1012 98 100 98 99 100 100 100 100 100 100 100 100* 100 100 100 100
STDY-6124877 98 99 98 99 100 100 100 100 100 100 100 100* 100 100 100 100
Newbould 99 100 99 99 100 100 100 61 100 100 100 100* 100 14* 100 100
G07I 99 100 99 99 100 100 100 100 100 100* 100 14* 100 100
ATCC 6538 99 99 99 99 100 100 100 100 100 100 100 100* 100 100 100 100
Lodi4R 99 * 100 99 97 100 99 100 100 100* 100 14* 100 100
VII M013 (ref.) + + * + +
M3386D 100 100 100* 100 100
MS4 100 100 100* 100 100
SA957 100 100 99* 100 100
HZW450 100 100 99* 100 100
VIII JKD6159 (ref.) + + + + + + + + * + * + + + +
IX ED133 (ref.) + + + + + + * + + + + + + + + + + + * + +
G42G 100 100 80 100 100 100 99* 100 100 100 100 100 100 100 100 100 100 100 100* 100 100
NCTC1803 100 100 100 100 100 100 99* 100 100 100 100 100 100 100 100 100 100 100 100* 100 100
NCTC9555 100 100 100 100 100 100 99* 100 100 100 100 100 100 100 100 100 100 57* 100* 100 100
O267 100 100 99 100 100 100 99* 100 100 100 100 100 100 100 100 100 100 100 100* 100 100
X O11 (ref.) + + * + + + +b + + * + * * +
O46 99 100 100* 93 100 100 100 100 99* 98 R * 100
XI G33O (ref.) + * + * + + + + + + + * + + + * + + + + + +
G68P 100 100* 100 99* 100 100 100 100 100 100 99 100* 100 100 100 99* 100 100 100 100 100 99
XII G11F (ref.) + + * + + + +c + + + + * + + + + + + + + + +
Strain 9 100 99 100* 100 100 100 100 100 100 100 99 100* 100 99 98 100 100 100 100 99 100 100
FKQO 100 100 100* 100 100 100 100 100 100 100 99 100* 100 100 100 100 100 100 100 99 100 100
GUATP222 100 100 100* 100 100 100 100 100 100 100 98 100* 100 100 100 100 100 100 45* 99 100 100
GUATP47 100 100 100* 100 100 100 100 100 100 100 98 100* 100 100 100 100 100 100 100 99 100 100
Ex1 scf 100 100 100* 100 100 100 100 100 100 100 99 100* 100 100 100 100 100 100 100 99 100 100
XIII G19F (ref.) + + + + + + + + + + +
st1332 100 100 100 100 100 100 99 100 100 100 100
NCTC8765 100 100 100 99 100 100 99 100 100 100 100
MSSA 100 99 100 99 100 100 99 100 100 100 100
strainNA 100 99 100 99 100 100 99 100 100 100 100
TSAR05 100 99 100 99 100 100 99 100 100 100 100
21334 100 99 100 99 100 100 99 100 100 100 100
K18 100 100 100 50* 100 100 99 100 100 100 100
RKI4 100 100 100 100 100 100 100 100 100 100 100
XIV G08M (ref.) + + + * + + + + + + + * + + + +
08-02300 100 100 100 R 100 100 100 100 100 100 100 99* 100 100 100 100
3688STDY6125053 100 100 100 100 100 100 100 100 100 100 100 99* 100 100 100 100
3688STDY6125046 100 100 100 100 100 100 100 100 100 100 100 99* 100 100 100 100
H1524 100 100 100 91* 100 100 100 100 100 100 100 99* 100 100 100 100
H1645 100 100 100 35* 100 100 100 100 100 100 100 99* 100 100 100 100
XV Lodi11bM (ref.) + + + + + + + + + +b + + * + + + +
4185 100 99 99 100 100 100 99 84 100 100 100 99 98* 100 14* 100 100
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a

For each vSaβ type, a reference (ref.) strain was chosen. The vSaβ genes were translated into protein sequences using the standard code and aligned to the corresponding protein of the reference strain. Shading indicates identity of ≥95%, and asterisks indicate truncated or fragmented genes. Where a gene was absent or truncated in the reference strain, the protein sequence of another strain of that type was used as a reference, indicated with R below the corresponding protein. +, presence of the corresponding genes in the reference strains that served as a target for comparing the genes of the other strains of the group.

b

vSaβ types X and XV have a second copy of SplB.

c

vSaβ type XII has a second copy of SplD4.

vSaβ type I (n = 11) originated mostly from humans, with the exception of 1 strain originating from a bovine mammary infection. vSaβ type I is found commonly and included well-studied clinical strains, such as strains N315, 502A, Mu3, Mu50, and JH1. vSaβ type II (n = 17) comprised 8 human isolates with the well-known strain COL and strain DSM 20231T. The remaining isolates originated from bovine mastitis. Of the vSaβ type III strains (n = 9), 7 originated from humans (including the hospital-acquired MRSA252 and the quality control strain ATCC 25923), and 2 strains were of unknown origin. All vSaβ type IV strains (n = 8) were isolated from bovine mastitis. Of vSaβ type V (n = 9), one host was unknown, and the other 8 strains originated from humans. vSaβ type VI (n = 11) were all isolated from either humans or cattle.

Only a few strains harbored vSaβ types VII to XI. vSaβ type VII (n = 5) contained 4 human isolates and 1 bovine mastitis isolate, and for vSaβ type VIII, only a single isolate was found. vSaβ types IX (n = 5) and X (n = 2) were uniquely isolated from ovine mastitis. The two vSaβ type XI strains were bovine mastitis strains. From vSaβ type XII strains (n = 6), 2 originated from cattle and 4 from humans. Out of 9 vSaβ type XIII strains, 5 originated from human hosts and 2 from cattle, and for 2 strains, no information was available. One vSaβ type XIV (n = 6) strain was isolated from Swiss cattle and 5 from human hosts. The two strains with type XV were both bovine mastitis isolates.

Structure of vSaβ and highly conserved regions.

All vSaβ islands were enclosed by 2 distinct genes coding for hypothetical proteins (hp genes). In the strain Mu50 (reference strain for vSaβ type I [see below]) the hp with the locus tag SAV1803 marks the 5′ terminus of vSaβ, and SAV1831 marks the 3′ terminus. These two hp genes could be found in nearly all vSaβ islands (see Fig. 1 and Table 1). For convenience, we refer to these sequences as sav1803 and sav1831, respectively. These hp genes were highly conserved among all strains, with product amino acid identities of >95% in SAV1803 and >98% in SAV1831. In all strains, sav1831 directly followed a cluster of 8 tRNAs (tRNAfMet, tRNAAsp, tRNAPhe, tRNAHis, tRNAGly, tRNAAsn, tRNAGlu, and tRNASer).

FIG 1.

FIG 1

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Representation of all Staphylococcus aureus genomic island vSaβ types I to XV, their virulence-associated genes, and other hypothetical genes located on vSaβ. For each vSaβ type, one reference strain is shown. Arrows show orientation of open reading frames. Asterisks indicate truncated or fragmented genes. Note that vSaβ IV is substantially longer due to the presence of a complete phage. All other sequences are scaled relative to each other.

Most vSaβ types followed a similar structural design (with exceptions of types V, VII, and XIII) carrying a distinct set of virulence genes (Fig. 1). They included hysA, the lipoprotein (lpn) genes, the RM genes hsdM and hsdS, a cluster of spl genes (splA, splB, splC, splD1, splD2, splD3, and splD4), a probable beta-lactamase gene (bla), a cluster of 9 bsa genes (bsaA1, bsaA2, bsaB, bsaC, bsaD, bsaE, bsaF, bsaG, and bsaP), the lukD and lukE genes, as well as an EGC consisting of the seg, sei, sem, sen, seo, and seu genes. In some cases, the seu gene was replaced by the two truncated genes, ϕent1 and ϕent2. The compositions of these genes and gene clusters varied substantially between different vSaβ types but were highly conserved within the same vSaβ type (Fig. 1 and Table 1). In addition to these core vSaβ genes, a number of hp genes were found of which some were assigned to a FIG number by the RAST (Rapid Annotations using Subsystem Technology) pipeline.

By typing the vSaβ islands and aligning all related sequences, a consensus structure for each vSaβ type could be defined. vSaβ type I possessed the genes of the type I RM system, an spl cluster (splA, splB, splC, and two copies of splD2), lukD, lukE, and a complete EGC. The amino acid sequences were, with only very few exceptions, highly similar within this vSaβ type (>95%) (Table 1). The exceptions were truncated genes, the lack of a second splD2 gene, and a lower sequence identity of the splD2 protein in three strains (Table 1). Furthermore, 3 sequences for mobile element proteins (mep), a bsaG fragment, bla, and remnants of phages were detected (Table 1; Fig. 1). Comparing the structures of all 11 vSaβ type I genomic islands, Mu50 showed consensus and hence was used as a reference for this vSaβ type. The consensus of vSaβ type II was best represented by S. aureus strain TW20. It harbored the type I RM genes, an spl cluster (splA, splB, splC, splD1, and two copies of splD2), a bla, a bsa cluster, and the lukD and lukE genes. Exceptions were some strains that harbored truncated hsdM, basE, and lukE genes. Moreover, vSaβ type II comprised sequences for two mep genes, bla, a number of hp genes, and a partial phage (Table 1). The consensus on vSaβ type III was strain MRSA252. It harbored hysA, hsdM, hsdS, an spl cluster (splB, splC, splD1, splD2, splD3, and splD4), and an EGC. Besides three spl genes being all pseudogenes (splB, splD2, and splD3), vSaβ type III lacked lukD and lukE but had additional genes in its 5′ region (a number of putative lpn genes, a hysA, and a lipase gene). vSaβ type III carried sequences for mep, hp, and remnants of a phage. In most of the type III strains, the core proteins were highly conserved, showing an identity of ≥99% when S. aureus strain MRSA252 was used as a reference. Deviations thereof could be found in the spl cluster and in hsdM (Table 1). The vSaβ type IV was best represented by strain M3783C and was, with over 85,000 bp, substantially longer than the other vSaβ types. It harbored a number of hp genes, a hysA gene, an spl cluster (splB, splC, splD2, splD4, and a truncated splA), a complete bsa cluster, an EGC (with truncated seg and sem), lukD, lukE, and the bla gene. hsdM and hsdS were either missing or had premature stop codons. The amino acid sequences of all other vSaβ key genes were highly conserved (≥99%) among strains, with only a small number of exceptions (Table 1). Furthermore, a 50.4-kb intact mosaic phage closest to Ipla88 (NCBI RefSeq accession no. NC011614) was detected in 6 vSaβ type IV strains, coding for approximately 75 proteins including those encoded by the EGC also found in other vSaβ types. In contrast, the strains M2323C and Sa110 harbored only remnants of two phages; hence, the vSaβ islands of these two strains were shorter than the other type IV islands. The structure of vSaβ type V differed substantially from the other types and was only 15,965 bp long and highly conserved, showing a nucleotide sequence identity of ≥99% when using strain S0385 as a reference. From the typical genes, vSaβ type V harbored only hysA. However, it carries a total of 4 transposons, as well as a number of hp genes, some of which were related to FIG numbers, or they were identified as lpn genes. Within vSaβ type VI, strain K2R was a suitable representative. It comprised hysA, hsdS, hsdM, an spl cluster (splA, slpB, splC, splD1, and two copies of splD2), bla, a bsaG fragment, lukD, lukE, a phage integrase, as well as a number of hp genes, some of which were assigned a FIG number, and others were identified as lpn genes. In addition, a partial phage was detected encompassing the entire spl cluster. Four strains (M0443, Newbould, G07I, and Lodi4R) had a frameshift in the beginning of lukE, leading to a truncated protein. Additionally, G07I and Lodi4R also lacked the splD1 and the second splD2 gene. Except for these very few special cases, all genes of vSaβ type VI were highly conserved between strains, showing an amino acid identity of ≥98%. The low overall nucleotide identity of 76% in strain Lodi4R compared to the reference was based on the insertion of the transposon Tn554 within hysA. This strain was, therefore, considered a special case of vSaβ type VI. Tn554 carried 3 transposase genes, as well as a bla operon carrying the blaI, blaR1, and blaZ. If Tn554 was disregarded, the nucleotide sequence identity increased to 93%. vSaβ type VII, similar to type V, was short (about 17,460 bp) and lacked the typical vSaβ genes, except for hysA and a hsdS fragment. Furthermore, all vSaβ type VII strains showed a partial phage with genes for proteins targeting the host’s immune response (chemotaxis-inhibiting protein Cpn and extracellular complement-binding protein Scn), as well as genes for a peptidase, a phage lysine, an ATP-binding protein, and a transposase. The amino acid sequences of these phage-encoded proteins were identical, with the exception of M3386D that was lacking a part of the phage containing genes coding for the ATP-binding protein and the transposase. The vSaβ type VIII included only the JKD6159 strain. It comprised hysA, hsdM, hsdS, an spl cluster (a truncated splA, splB, splC, splD1, and splD4), bla, a bsaG fragment, lukD, lukE, a partial phage, a number of hp and phage-related genes, and a number of lpn genes in the 5′ region. For vSaβ type IX, ED133 served as the reference harboring the vSaβ key genes hsdM and hsdS, an spl cluster (a truncated splA, slpB, splC, and splD2), lukD, lukE (truncated), bla, and a complete bsa cluster, as well as a number of hp genes, some of which were identified as lpn genes and others that were assigned a FIG number. In addition, vSaβ type IX carried 2 genes encoding ATP-binding cassette (ABC) transporter proteins. and a gene encoding a phage integrase. Despite the isolates being from 3 different countries, they shared an overall nucleotide sequence identity of ≥95% in their vSaβ. The only small difference in the vSaβ key genes was found in hsdM and lukD, which showed lower similarities in some strains (Table 1). vSaβ type X showed 93% nucleotide sequence identity. The vSaβ consisted of an spl cluster (splA, two copies of splB, splC, splD1, splD2), a bla, a bsaG fragment, lukD, lukE, a complete hsdS gene and an hsdM gene with a premature stop codon. In addition to a number of hp and FIG-assigned genes, lpn, two genes encoding an ABC transporter, and a phage integrase gene could be found, similar to vSaβ type IX. The amino acid identity in the key genes were ≥98%, with the exceptions of lukE being truncated in O11, splD1 lacking in strain O46, and a slightly lower identity in splD2 (93% amino acid identity). vSaβ type XI consisted of two strains with an amino acid identity of ≥99% in the vSaβ key genes and a nucleotide identity of 99% over the entire vSaβ region. Their vSaβ consists of hsdM and hsdS, but hsdM has a premature stop codon. Furthermore, vSaβ type XI was characterized by an spl cluster (splA, splB, splC, splD2, splD3, and splD4), a bla, a basG fragment, lukD, lukE, a complete EGC (with a truncated sen), several hp genes (some of which with assigned FIG numbers), and a short hysA fragment. The most striking feature of vSaβ type XII was the enlarged spl cluster consisting of splA, splB, splC, splD1, splD2, splD3, and 2 copies of splD4. It further harbored both RM genes (hsdM with premature stop codon), bla, a bsaG fragment, lukD, lukE, and a complete EGC along with several hp genes, some with assigned FIG numbers. The strains categorized as vSaβ type XIII had a typical vSaβ structure in the 3′ end, yet a clear beginning could not be defined for this vSaβ type, as it lacked the conserved 5′-end sav1803. Instead, there were three hp genes as well as two recently discovered enterotoxin sequences, sel26 and sel27. These were followed by several hp genes (some with assigned FIG number) and genes typical for vSaβ, such as lpn, hsdM, hdsS, bla, the EGC followed by the tRNA cluster, and sav1831, typically marking the 3′ end. vSaβ type XIV harbored hysA, both RM genes (hsdM and hsdS), an spl cluster (splA, splB, splC, splD1, splD2, and splD4), bla, a bsaG fragment, lukD, and lukE. When strain G08M was used as a reference, most proteins showed an amino acid identity of 100%, with the exceptions being three strains with truncated hsdM genes. vSaβ type XV consisted of both RM genes (hsdM and hsdS), an spl cluster (splA, two copies of splB, splC, splD1, two splD2, splD3, and splD4), bla, a bsaG fragment, lukD, and lukE. When strain Lodi11bM was used as a reference, the only differences were lukE being truncated and SplD4 having a lower amino acid identity in strain 4185.

In summary, all vSaβ types had the same basic structural design. The design was minimal for types V, VII, and XIII and was more complex for the other types, as they harbored a distinct set of virulence factors and gene clusters. Also common to all vSaβ types, except to type XIII, was the pervasive flanking by the same conserved sequences with the locus tags SAV1803 and SAV1831. Variation among the vSaβ types was observed for the spl cluster in gene number and composition. Furthermore, some types were characterized by truncated RM genes and/or bsa or spl genes. Within each vSaβ type, they were highly conserved, at the structural and the protein levels.

HsdS.

Inspection of the HsdS sequences with a phylogenetic approach (Fig. 2A) revealed that the HsdS sequence alone does not provide sufficient resolution for inference of gene content or virulence determinants of the entire vSaβ. Hence, categorization of vSaβ based on the nucleotide sequence identity of the entire genomic island can be considered the method of choice when the entire sequence of the genomic island is available.

FIG 2.

FIG 2

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(A and B) Phylogeny of the 20 HsdS (A) and 68 Spl (B) amino acid sequences of Staphylococcus aureus. For both trees, protein sequences that differedin at least one amino acid were aligned, and a phylogenetic tree was reconstructed using maximum likelihood. For each tree, a scale indicates the relative distance on the phylogenetic tree. (A) The blue line indicates a strain’s vSaβ type, and its clonal complex (CC) is given in parentheses. (B) The strain from which the amino acid sequence originated is shown in parentheses. Numbers in red represent bootstrap values. Question marks indicate sequences of Spl with previously uncertain or unknown nomenclature, and red and blue lines show suggested nomenclature of the corresponding branch based on phylogenetic distances of the Spl amino acid sequences. Curated sequences are marked with an asterisk.

Serine proteases.

All vSaβ islands, with the exceptions of types V, VII, and XIII, carried an spl cluster, harboring between 4 and 9 spl genes. Phylogenetic analyses using the maximum-likelihood method confirmed the existing Spl family members SplA, SplB, SplC, and SplD, but with SplD having 4 different variants (SplD1, SplD2, SplD3, and SplD4) (Fig. 2B). SplD1 replaces the former SplE, and SplD2 replaces the former SplD and SplF. The SplD3 and SplD4 clades include Spl proteins that could not be assigned into any of the preexisting Spl variants. Furthermore, the presence of multiple splD variants per strain points to a relatively recent gene duplication.

Clonal complexes and spa types.

spa typing showed that one vSaβ type can harbor a number of spa types, but each spa type is limited to one vSaβ type. Highly striking was the consistency of a vSaβ type with the strains’ clonality. The predominance of a single clonal complex (CC) per vSaβ type underlined the concept that vSaβ acquisition and its diversification happened prior or simultaneously to the clonal diversification of ancestral S. aureus strains.

DISCUSSION

With the rapid advancement of sequencing technologies and the decreasing costs thereof, the amount of S. aureus sequences deposited in databases is growing exponentially. These databases offer a powerful information source for studying the variabilities and consistencies between different isolates and their potential ability to cause disease. As opposed to the slow accumulation of point mutations, acquisition of larger parts of DNA through horizontal gene transfer leads to a rapid genetic change. This can be crucial in survival under certain selection pressures (antibiotics) or in novel niches (new host) (10, 12, 13). The mechanism of how S. aureus acquired its genomic islands is not fully understood, yet here, we have elaborated why phage mediation has played a crucial role.

Viral origin of vSaβ.

Throughout all vSaβ types, the strains belonging to one vSaβ type almost exclusively harbor the same virulence genes. In addition to the gene composition, an amino acid sequence identity of 95% or higher is another characteristic within each type, and in many cases, even 100% over all strains of a vSaβ type (Table 1).

Despite the range of hosts and geographical origins covered in this study, the vSaβ islands were always located in between SAV1803 and SAV1831 at the 5′ and 3′ ends, respectively. This highly precise location together with the tRNA cluster preceding SAV1831 as observed throughout all vSaβ types strongly indicates that these sites may be key for the presence of the vSaβ islands in S. aureus. Indeed, tRNAs are known to harbor a conserved attachment site (14) for integrating prophages and other foreign DNA (15, 16).

It is widely accepted that S. aureus genomic islands were acquired through horizontal gene transfer, but the exact mechanism and their current mobility status have been questioned (8, 10, 17). Our data show the presence of partial phages in almost all vSaβ types, with the exceptions of type V, where no phages were predicted, and type IV, where a complete prophage was predicted by PHASTER. In addition, vSaβ type IV also harbors all virulence-related key features of vSaβ, as follows: the type I RM system, the spl cluster, the bsa cluster, the leukocidin genes, and the EGC. Other vSaβ types possess the EGC only (types I, III, XI, XII, and XIII), the bsa cluster only (II and IX), or lack both. In this sense, vSaβ type IV can be considered the most complete of all vSaβ types regarding virulence-related gene content and phage. Moon et al. (14) showed that this very phage of strain RF122 (vSaβ type IV) can be mobilized in vitro, resulting in heterogeneous, yet overlapping particles that integrated sequentially through recombination into the host; in some cases, it even resulted in the transfer of the almost-complete vSaβ (14).

Our results also demonstrate that all vSaβ islands containing the key virulence factors also harbored partial phages, suggesting that vSaβ originated in an ancestral S. aureus strain through a phage integration event. In the course of evolution, multiple recombination, integration, and excision events may have occurred, resulting in various combinations of the virulence genes that we now observe in the different vSaβ types. To our knowledge, vSaβ type IV is the only type to harbor a complete phage that has been shown to be mobilized, suggesting that the phages of the other vSaβ types are likely to have lost their mobility in the course of evolution.

The unequivocal location, the demonstrated mobility (14), and the ubiquitous presence of phage particles in vSaβ islands, as well as a conserved phage attachment site, strongly support the hypothesis that vSaβ was mediated by a phage, followed by diversification into the types observed today.

vSaβ and clonality.

In total, vSaβ typing of 103 S. aureus strains revealed 15 different types. They correlated strongly with the CCs and were always linked to one specific vSaβ type. Therefore, the CC of S. aureus allows the link to virulence-associated vSaβ key genes. This link has been made previously (18), but by assessing and categorizing the novel 12 vSaβ types, we confirmed this observation to be a general principle. Indeed, all studied strains follow this pattern, indicating that the horizontal acquisition of vSaβ in an ancestral S. aureus strain happened before divergence into the clones occurred. It is evident that the primordial vSaβ underwent multiple genetic changes (e.g., recombination and duplications), likely just prior or simultaneously to clone formation, resulting in the types observed today.

The evolutionary link of vSaβ types and CCs are in perfect agreement with the topology of the phylogenetic tree by Boss et al. (19). For that study, the authors concatenated 7 S. aureus-specific genes from 30 different strains and aligned these using the Needleman-Wunsch algorithm. This alignment was then used to construct a maximum parsimony phylogeny. This phylogeny showed the evolutionary relationship between some of the most common S. aureus CCs that all possess a vSaβ and a type-specific set of virulence genes. Therefore, it is evident that the common ancestor of these CCs already carried an ancestral vSaβ.

The discrepancy between the overall structures of vSaβ types V and VII and all other vSaβ types can be explained by an evolutionary loss of the vSaβ key genes in these types during two separate events. We can still find vSaβ typical elements, such as the conserved regions marking the 5′ and 3′ ends of vSaβ or the almost ubiquitous hp FIG01108826 in the 3′ region. Furthermore, type V harbors sequences that can be found in a number of other vSaβ types, such as 3 hp genes with an assigned FIG number located the 5′ region (FIG01108398, FIG01108644, and FIG01108514), lpn, and hysA. Interestingly, type VII encodes different virulence factors that tackle the host’s immune defenses. As vSaβ types V and VII have additional transposase genes, we suggest that in these two types, the vSaβ key genes were replaced by a transposon, indicating that vSaβ themselves may be a hot spot for inserting mobile genetic elements and potentially accumulating virulence factors.

vSaβ type XIII has a deviate structure, as it lacks the typical 5′ region found in the other types. Together with types V and VII, it lacks the spl cluster and the luk genes. We cannot exactly locate the beginning of this vSaβ type on the genome, but interestingly, we found two very recently discovered phage-associated enterotoxin genes (sel26 and sel27) in that region (20). Furthermore, we found an hp (FIG01108398) which is adjacent to the SAV1803 in some other types.

Virulence and vSaβ.

The variable regions contribute to the fate of an S. aureus strain on a given host and its disease-causing potential, as these regions encode a number of virulence factors (6, 10). They include the pore-forming LukD and LukE, which are present on most vSaβ types (except III, V, VII, and XIII). Both proteins are members of the leukocidin family (21) that form pores in the lipid bilayer of host cells, particularly in neutrophils, leading to cell death and, therefore, the promotion of immune evasion and progression of the infection (22, 23).

Further virulence factors encoded on vSaβ are the Spl proteins that are unique to S. aureus and are organized in an operon (11). vSaβ types V, VII, and XIII lack the spl cluster, whereas all other types have a spl cluster, some of them with truncated genes. Based on our phylogenetic approach, there are 4 different spl genes (splA, splB, splC, and splD) and 4 gene variants of splD (splD1, splD2, splD3, and splD4). SplA, SplB, and SplC form their own clades and are clearly separated from the SplD clade (Fig. 2B). The former SplD and SplF are now members of the SplD2 clade, which is unsurprising, as their amino acid identity can be as high as 94% (11, 24). The SplD3 and SplD4 clades consist both of Spl sequences that have not been previously studied and did not match any of the predefined spl genes based on sequence similarity. We observed a high conservation of the spl operon within but not between vSaβ types. The function of spl genes in infection and disease is still largely unclear (24–27). Studies showed that spl genes are expressed and secreted during host infection (25, 27) and have been linked to allergic reactions (28, 29). Interestingly, vSaβ can harbor multiple copies of the very same spl gene. The plasticity of the spl cluster is an evidence for its varied importance among different vSaβ types.

Hyaluronic acid is a major component of the connective tissue, in particular, the extracellular matrix (30). HysA is considered a virulence factor, as it can depolymerize hyaluronic acid and favoring the spreading of infection (31, 32). Many S. aureus genomes contain a chromosomal copy of hysA (33) outside of vSAβ. The additional copy present in the vSaβ region of types III to VIII and XIV may be linked to enhanced invasiveness of these clones.

Lantibiotics (or bacteriocins) are antimicrobial peptides produced by some Gram-positive bacteria against closely related species (34, 35) and are thought to play a role in colonization by outcompeting other bacteria (10, 24).

vSaβ typing.

In contrast to the link of CC and vSaβ types, it has been suggested that the vSaβ type is dependent on the strain’s HsdS sequence (8). These predictions were not very reliable, as they were based on 3 vSaβ types and 12 sequenced S. aureus strains only. From a total of 15 vSaβ types, we defined 12 new types based on multiple hosts and geographical regions. Within the scope of our study, the method used for typing the vSaβ region proved to be a very robust procedure, as all used strains could be exclusively allocated to one vSaβ type, and cross-classification was never observed. Hence, we expect the specified principles to be robust enough to uphold future findings when additional S. aureus strains are analyzed.

In the scope of this study, we limited the data set to clinically relevant S. aureus strains either from human or animal infections. Strains that were not invasive (i.e., colonizers and strains isolated from foods) were excluded from the data set. Hence, our results are limited to these invasive strains only, yet we did find that a few colonizers also fit into the system proposed here (data not shown). In the future, more vSaβ regions need to be analyzed spanning more hosts and including noninvasive strains.

Conclusions.

In general, the vSaβ islands harbor a number of virulence-associated and pathogenic genes with different scopes of action. While the exact functions of many of these genes are yet to be unraveled, it is clear that they have severe effects on the host’s health and are likely to play key roles in S. aureus adaptation to the clinical microenvironment. Our data support a viral origin of the vSaβ region. As the vSaβ type is strongly linked to a strain’s CC, acquisition of the vSaβ region happened in a very ancestral S. aureus strain while the transformation to the distinct vSaβ subtypes occurred before or simultaneously to diversification into the different clones. The here-suggested superordinate system to classify S. aureus strains based on their vSaβ region may be used in the future to assess the clinical potential of an S. aureus strain. In the future, more vSaβ genomic islands need to be analyzed and categorized into this superordinate system.

MATERIALS AND METHODS

Sequencing.

The in-house S. aureus genome collection includes 23 strains that were previously sampled from bovine mastitis (36). All strains were kept in skim milk at –20°C and were recultured at 37°C for 24 h on blood agar (bioMérieux Suisse s.a., Geneva, Switzerland). Plates were sent to Microsynth AG (Balgach, Switzerland) for DNA extraction and subsequent whole-genome sequencing (WGS), initially by the 454 (Roche, Basel, Switzerland) and later by the Illumina (Illumina, Inc., San Diego, CA) technology, as the 454 method was no longer available. For de novo assembly of the reads to contigs, they used the Newbler v.2.6 assembler for the 454 (Roche) technology and SPAdes v.3.1 (37) for the Illumina technology (see File S1 in the supplemental material for details). In addition, contigs of 4 bovine S. aureus strains after Illumina WGS were provided by M. Luini (IZSLER, Lodi, Italy) and P. Cremonesi (CNR, Lodi, Italy).

Data collection.

In an attempt to characterize the vSaβ islands of our 27 bovine mastitis S. aureus genomes as described by Baba et al. (8), it turned out that many of them did not match the 3 previously defined types proposing a much higher diversity of vSaβ; hence, an in-depth characterization based on more data was required. To do so, more sequences were collected by a BLAST search (36) of each known, presumptively novel, vSaβ against the NCBI nonredundant/nucleotide (nr/nt) database (https://www.ncbi.nlm.nih.gov/nucleotide/) using the default BLAST settings and limiting the search to S. aureus. To increase the diversity of the vSaβ islands, the NCBI databases were also evaluated for genomes or contigs of clinical S. aureus strains from hosts other than humans or cattle. This approach was selected, as invasive strains of S. aureus are host specific (19, 38, 39), possibly accounting for additional types of vSaβ islands. For each vSaβ type, ≥10 chromosomal sequences of clinical S. aureus were then attempted to be retrieved. If this was impossible, the BLAST search was further extended to the NCBI whole genome shotgun contig database (https://www.ncbi.nlm.nih.gov/assembly/). All available chromosomal sequences were then retrieved, including all contigs containing a complete vSaβ region.

Using this approach, a total of 76 sequences were obtained, with 43 sequences from genomes and 33 sequences from contigs (see File S2).

Data analysis.

(i) Data set. In total, 27 of our own and 76 publicly available sequences were included in the present study. From these 103 analyzed S. aureus strains, 58 originated from human hosts, 33 were from bovine hosts, and 7 were from ovine hosts. For 5 strains, no host information was available. Of these 103 strains, 37 strains showed known vSaβ types (I to III), and 66 strains showed novel vSaβ types (IV to XV) (see File S2 for details).

(ii) vSaβ typing. The vSaβ regions were identified on genome sequences by aligning the sequences of conserved hp genes flanking vSaβ at the 5′ end (SAV1803) and 3′ end (SAV1831) (8) using the Clone Manager Professional 9 (CM9) software (Scientific & Educational Software, Denver, CO). Subtyping of the vSaβ islands was then based on the initial work by Baba et al. (8). Using the hsdS gene of 12 clinically relevant S. aureus strains, Baba et al. grouped them into three vSaβ types (vSaβ I, vSaβ II, and vSaβ III [8]). We then aligned the vSaβ sequences of these 12 strains in the CM9 software using the Needleman-Wunsch algorithm and found that the overall similarities within a vSaβ type were ≥90%. For each of these vSaβ types, a representative sequence was then selected and considered the type-specific reference sequence (Table 1). Disregarding included phages and transposons, vSaβ islands showing overall sequence similarities of <90% compared to all the existing reference sequences were then considered new vSaβ types. Through the iterative process of aligning nontyped vSaβ islands to each of the vSaβ type reference sequences, the 103 sequences of the data set were grouped into 15 vSaβ types (vSaβ I to XV, Table 1).

(iii) HsdS phylogenetics. To infer the phylogeny of the HsdS proteins (37, 40), the corresponding nucleotide sequences were translated using the standard code. Proteins that differed in at least one amino acid were then used for a multiple-sequence alignment (MSA) in the CM9 software using the Needleman-Wunsch approach. The MSA was exported to the BioEdit software (http://www.mbio.ncsu.edu/BioEdit/bioedit.html) for visual inspection and manual curation. Afterwards, the curated MSA was imported into the MEGA X software (41) to assess a maximum likelihood (ML) phylogeny of the HsdS proteins. To do so, models using 9 different substitution matrices were computed including or leaving out modeling for invariant sites and for the evolutionary rate differences among sites by a discrete gamma distribution. The optimal model was then selected based on the lowest value of the Akaike information criterion resulting in the JTT substitution matrix (37), with specific parameters for the gamma distribution. This model was then used to construct a phylogenetic ML tree (MEGA X software [41]). Initial trees were built by applying the maximum parsimony algorithm. The topology with the highest log-likelihood value was then selected (Fig. 2A). Strains lacking the hsdS gene were excluded.

(iv) Multilocus and spa typing. Multilocus sequence typing (MLST) of seven housekeeping genes (42) and spa typing (43) were performed for all S. aureus genomes using the Center for Genomic Epidemiology online platform (http://www.genomicepidemiology.org/). In the eBURST V3 program, the sequence types (STs) resulting from the MLST were used to allocate each strain to a clonal complex (CC).

(v) Annotations. Annotations were assigned to the vSaβ regions using the RAST pipeline (44–46). RAST annotations of all genes (including hp genes with or without a FIG number assigned by the RAST pipeline) were verified by a BLAST search against the UniProt database (47). Where necessary, annotations were corrected manually based on the UniProt data; whenever possible, reviewed entries were used. If the RAST pipeline failed to annotate genes known to be located on vSaβ, these genes were searched manually by aligning the nucleotide sequences of the well-characterized strains N315 and MW2 to the vSaβ of interest. Transfer RNAs were identified by tRNAscan-SE 2.0 (48).

(vi) Phage detection. Phages and phage remnants were identified by the online software Phage Search Tool Enhanced Release (PHASTER) (49, 50). To ensure detection, regions 5,000 bp up- and downstream of vSaβ were included in the analysis.

(vii) Serine protease nomenclature. All complete and curated spl sequences were analyzed and translated into proteins using the standard code. Sixty-eight amino acid sequences were unique (i.e., differed in at least one amino acid) and were used to reconstruct the phylogeny using the same methods as mentioned above for the HsdS protein sequence (ML method, JTT substitution matrix, discrete gamma distribution to model evolutionary rate differences among sites) (Fig. 2A and B). As the current nomenclature of spl does not account for the phylogenetic relationship between their sequences and in some cases is ambiguous and arbitrary, a new nomenclature was developed using the ML phylogenetic approach based on the protein sequences. Starting from the root of the phylogenetic tree, each node along a branch is followed until a node forming a monophyletic clade is reached whose branch connecting to its predecessor is characterized by a bootstrap value (BT) of ≥95 and a phylogenetic distance (d) of >0.10. All leaves forming this clade obtain the same letter (e.g., “D”) added to the basic name of the protein (in this case “Spl”), resulting in the name SplD for all of these leaves. The branch is then followed toward the leaves. If a node is reached forming a monophyletic subclade whose branch connecting to the predecessor is characterized by a BT of ≥95 and d of >0.10, the leaves of this subclade are labeled by adding a number (e.g., “2”), resulting in the name SplD2. At this stage, further evaluation toward the leaves is stopped. If only one branch of a bifurcation fulfills these conditions, the subclade formed by the nonfulfilling branch gets a new letter or number as well.

Data availability.

All whole-genome projects have been deposited in the NCBI database under the BioProject number PRJNA531079 with the accession numbers SZYI00000000 (Lodi13K), SZYM00000000 (G12B), SZYO00000000 (G29N), SZYT00000000 (Lodi10B), VCQP00000000 (M2084B), SZYY00000000 (M2130B), SZZA00000000 (M2529B), SZZF00000000 (M5512B), VCQQ00000000 (M5171B), SZZG00000000 (M6020B), SZZE00000000 (M3783C), SZZC00000000 (M2839C), SZYW00000000 (M1280C), SZYX00000000 (M1655C), SZYZ00000000 (M2323C), SZZB00000000 (M2682A), SZYS00000000 (K2R), SZYJ00000000 (G07I), SZYV00000000 (Lodi4R), SZZD00000000 (M3386D), SZYQ00000000 (G42G), SZYP00000000 (G33O), SZYR00000000 (G68P), SZYL00000000 (G11F), SZYN00000000 (G19F), SZYK00000000 (G08M), and SZYU00000000 (Lodi11bM).

Supplementary Material

Supplemental file 1
JB.00777-18-s0001.xlsx (12.3KB, xlsx)
Supplemental file 2
JB.00777-18-s0002.xlsx (58.1KB, xlsx)

ACKNOWLEDGMENTS

We thank Mario Luini of the Instituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (ISZLER, Lodi, Italy) and P. Cremonesi of the Istituto di Biologia e Biotecnologia Agraria (CNR, Lodi, Italy) for providing sequencing data.

This work was financially supported by the Agroscope research program AFP REDYMO.

Footnotes

Supplemental material for this article may be found at https://doi.org/10.1128/JB.00777-18.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplemental file 1
JB.00777-18-s0001.xlsx (12.3KB, xlsx)
Supplemental file 2
JB.00777-18-s0002.xlsx (58.1KB, xlsx)

Data Availability Statement

All whole-genome projects have been deposited in the NCBI database under the BioProject number PRJNA531079 with the accession numbers SZYI00000000 (Lodi13K), SZYM00000000 (G12B), SZYO00000000 (G29N), SZYT00000000 (Lodi10B), VCQP00000000 (M2084B), SZYY00000000 (M2130B), SZZA00000000 (M2529B), SZZF00000000 (M5512B), VCQQ00000000 (M5171B), SZZG00000000 (M6020B), SZZE00000000 (M3783C), SZZC00000000 (M2839C), SZYW00000000 (M1280C), SZYX00000000 (M1655C), SZYZ00000000 (M2323C), SZZB00000000 (M2682A), SZYS00000000 (K2R), SZYJ00000000 (G07I), SZYV00000000 (Lodi4R), SZZD00000000 (M3386D), SZYQ00000000 (G42G), SZYP00000000 (G33O), SZYR00000000 (G68P), SZYL00000000 (G11F), SZYN00000000 (G19F), SZYK00000000 (G08M), and SZYU00000000 (Lodi11bM).

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