Skip to main content
. 2019 Oct 15;8:e49853. doi: 10.7554/eLife.49853

Figure 2. The recently amplified WRAP propeller in Npun_R6612 of Nostoc punctiforme PCC73102.

(A) Multiple sequence alignment of the 14 blades of WRAP. Non-identical repeats are colored in red and the non-repeating β-strand 4 of the velcro blade is underlined. The four β-strands of the propeller blades are indicated above the alignment. The repeat unit chosen for in vitro studies is highlighted by a box. (B) Crystal structure of the WRAP propeller (PDB 2YMU). (C) Oligomerization of WRAP repeats. Assembly was probed by crosslinking proteins with 0.6% glutaraldehyde (GA) and subsequent analysis by SDS-PAGE and Coomassie Blue G250 staining. On the left side, non-crosslinked proteins are shown for comparison.

Figure 2.

Figure 2—figure supplement 1. Sequences of WRAP constructs.

Figure 2—figure supplement 1.

All constructs start with an additional GAMG after TEV-cleavage.
Figure 2—figure supplement 2. Purification of WRAP fragments.

Figure 2—figure supplement 2.

Shown are the elution profiles of the Superdex 75 10/300 gel size-exclusion column runs.