Abstract
The Sysmex XE‐2100® (Sysmex Corp. Kobe, Japan) is a latest‐generation hematology analyzer. Its optical and electrical measuring technology is improved by the addition of flux cytometry, fluorescence, and differential lysis. Its analytical performance in terms of precision, reproducibility, linearity, carryover, and time stability was found to be entirely satisfactory. In addition, the results of 500 complete blood counts and differentials correlated perfectly with those obtained by the Coulter STKS® (Beckman Coulter, Villapointe, France). The comparison of 500 leukocyte differential count results analyzed in parallel with optical microscopy and the XE‐2100® were surprising, and favorable to the XE‐2100®. This analyzer provides the user with an undeniable feeling of security concerning its reliability in detecting and identifying anomalies in the automated leukocyte differential count. With a sensitivity of 96%, a negative predictive value (NPV) of 98%, and a false‐negative (FN) rate of 4%, the XE‐2100® has perhaps reached the technological limits for a machine performing morphological recognition of normal and pathological blood cells. J. Clin. Lab. Anal. 17:113–123, 2003. © 2003 Wiley‐Liss, Inc.
Keywords: hematology analyzer, Sysmex XE‐2100®, Coulter STKS®, performance evaluation
REFERENCES
- 1. Ruzicka K, Veitl M, Thalhammer‐Scherrer R, et al. The new hematology analyzer Sysmex XE‐2100: performance evaluation of a novel white blood cell differential technology. Arch Pathol Lab Med 2001;125:391–396. [DOI] [PubMed] [Google Scholar]
- 2. Briggs C, Harrison P, Grant D, et al. New quantitative parameters on a recently introduced automated blood cell counter–the XE 2100. Clin Lab Haematol 2000;22:345–350. [DOI] [PubMed] [Google Scholar]
- 3. Schwartz D. Méthode statistique à l'usage des médecins et des biologistes. Paris: Flammarion Médecine Science; 1986; p 307.
- 4. England JM, Rowan RM, Van Assendelft OW, et al. Protocol for evaluation of automated blood cell counters. International Committee for Standardization in Haematology (ICSH). Clin Lab Haematol 1984;6:69–84. [DOI] [PubMed] [Google Scholar]
- 5. Ferrero‐Vacher C, Maerfeld K, Fraye M, et al. Évaluation du Cell‐Dyn 4000® Abbott automate d'hématologie. Rev Fr Lab 2002;340:53–64. [Google Scholar]
- 6. Ollier L, Maerfeld K, Ferrero‐Vacher C, et al. Évaluation de l'automate d'hématologie Sysmex SE 9500® . Rev Fr Lab 2001;333:33–42. [Google Scholar]
- 7. Ferrero‐Vacher C, Sudaka I, Jambou D, et al. Evaluation of the ABX Cobas Vega automated hematology analyzer and comparison with the Coulter STKS. Hematol Cell Ther 1997;39:149–158. [DOI] [PubMed] [Google Scholar]
- 8. Maerfeld K. Évaluation comparative des automates haut de gamme d'hématologie au Centre Hospitalier Universitaire de Nice. Ph.D. thesis, University of Nice, Nice, France; 1999.
- 9. Erwa W, Bauer FR, Etschmaier R, et al. Analysis of aged samples with the Abbott CD4000 hematology analyzer. Eur J Lab Med 1998;6:4–15. [Google Scholar]
- 10. Sandhaus LM, Osei ES, Agrawal NN, et al. Platelet counting by the Coulter LH 750, Sysmex XE 2100, and Advia 120: a comparative analysis using the RBC/platelet ratio reference method. Am J Clin Pathol 2002;118:235–241. [DOI] [PubMed] [Google Scholar]