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. 2005 Nov 21;19(6):253–259. doi: 10.1002/jcla.20087

Urinary Protein Analysis in Pre‐ and Postoperative Cancer Patients

Kayo Yokomizo 1,✉, Shiro Iijima 2, Nobue Sakai 3, Yuriko Kurihara 1, Nobuo Hitratsuka 1, Kagami Nagai 4, Takehisa Iwai 4, Kiyoko Shiba 1
PMCID: PMC6807788  PMID: 16302210

Abstract

Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Analyses were performed on days–1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided into nine fractions. The main proteins in the fractions (Fr.) were identified as follows: immunoglobulin G in Fr. A, Tamm‐Horsfall glycoprotein (THP) in Fr. B, transferrin in Fr. C, albumin in Fr. D, α1‐acid glycoprotein in Fr. E, α1‐microglobulin in Fr. F, retinol binding protein in Fr. G, and β2‐microglobulin in Fr. I. The protein in Fr. H was not identified. The percentage of each fraction was calculated from the densitometry pattern of each lane. The percentage values were averaged among all the patients, and pre‐ and postoperative data were compared. The percentage of Frs. E, F, and G increased on days 1–7, and the changes in these three proteins were similar to changes in serum C‐reactive protein (CRP). In particular, the percentage of Fr. G peaked within 1 day of operation, which was faster than for CRP. Conversely, other fractions decreased. These results suggest that urinary protein analysis is useful for monitoring the response to surgical stress. J. Clin. Lab. Anal. 19:253–259, 2005. © 2005 Wiley‐Liss, Inc.

Keywords: sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, densitometry, fraction percentage, α1‐acid glycoprotein, α1‐microglobulin, retinol binding protein

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