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Journal of Clinical Laboratory Analysis logoLink to Journal of Clinical Laboratory Analysis
. 2002 Mar 15;16(2):115–120. doi: 10.1002/jcla.10033

Analysis of 3‐hydroxydodecanedioic acid for studies of fatty acid metabolic disorders: Preparation of stable isotope standards

JS Chickos 1, Barbara A Way 2, J Wilson 1, M Shaharuzzaman 1, J Laird 1, M Landt 2,
PMCID: PMC6807789  PMID: 11948802

Abstract

Current diagnostic tests to detect disorders of fatty acids metabolism, such as long‐chain hydroxyacyl CoA dehydrogenase deficiency (LCHAD), are hampered by insensitivity or a long delay time required for results. Children with LCHAD deficiency are known to excrete 3‐hydroxydicarboxylic acids with chain lengths of 10–16 carbons, but a quantitative method to measure excretion of these potentially diagnostically important compounds has not been reported. We report synthetic schemes for synthesis of 3‐hydroxydodecanedioic acid and a di‐deuterated analog, suitable for use in a stable‐isotope dilution mass spectrometric analytical approach. Evaluation of several common derivatization protocols to produce a volatile derivative for gas chromatography determined that trimethylsyl derivatives produced the best efficiency and stability. Positive‐ion chemical ionization mass spectrometry provided the greatest yield of characteristic ions. These results indicate the basic reagents needed to develop sensitive and accurate 3‐hydroxydodecanedioic acid measurements for diagnosis of LCHAD deficiency and other fatty acid oxidation disorders. J. Clin. Lab. Anal. 16:115–120, 2002. © 2002 Wiley‐Liss, Inc.

Keywords: metabolic genetic disease, mass spectroscopy, diagnosis

REFERENCES

  • 1. Bennett MJ. 1990. The laboratory diagnosis of inborn errors of mitochondrial fatty acid oxidation. Ann Clin Biochem 27:519–531. [DOI] [PubMed] [Google Scholar]
  • 2. Millington DS, Roe CR, Maltby DA. 1984. Application of high resolution fast atom bombardment and constant B/E ratio linked scanning to the identification and analysis of acylcarnitines in metabolic disease. Biomed Mass Spectrom 11:236–241. [DOI] [PubMed] [Google Scholar]
  • 3. Rinaldo P, O’Shea JJ, Coates PM, Hale DE, Stanley CA, Tanaka K. 1988. Medium‐chain acyl‐CoA dehydrogenase deficiency. Diagnosis by stable‐isotope dilution measurement of urinary n‐hexanoylglycine and 3‐phenylpropionylglycine. N Engl J Med 319:1308–1313. [DOI] [PubMed] [Google Scholar]
  • 4. Ibdah JA, Tein I, Dionisi‐Vici C, et al. 1998. Mild trifunctional protein deficiency is associated with progressive neuropathy and myopathy and suggests a novel genotype‐phenotype correlation. J Clin Invest 102:1193–1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5. Hagenfeldt L, Venizelos N, von Döbeln U. 1995. Clinical and biochemical presentation of long‐chain 3‐hydroxyacyl‐CoA dehydrogenase deficiency. J Inher Metab Dis 18:245–248. [DOI] [PubMed] [Google Scholar]
  • 6. Jones PM, Quinn R, Fennessey PV, et al. 2000. Improved stable isotope dilution‐gas chromatography‐mass spectrometry method for serum or plasma free 3‐hydroxy‐fatty acids and its utility for the study of disorders of mitochondrial fatty acid β‐oxidation. Clin Chem 46:149–155. [PubMed] [Google Scholar]
  • 7. Noller CR, Adams R. 1926. Synthesis of a homolog of chaulmoogric acid. Δ2‐cyclopentenylacetic acid. J Am Chem Soc 48:1074–1080. [Google Scholar]
  • 8. Goddard AE, Ward JB. 1922. Metallic derivatives of nitrophenolic compounds. Part IV. Some complex nitrophenoxides of magnesium, silver, and lead. J Chem Soc 121:262–266. [Google Scholar]
  • 9. Tserng K‐Y, Jin S‐J. 1991. Metabolic origin of urinary 3‐hydroxy dicarboxylic acids. Biochemistry 30:2508–2514. [DOI] [PubMed] [Google Scholar]
  • 10. Bennett MJ, Weinberger MJ, Sherwood WG, Burlina AB. 1994. Secondary 3‐hydroxydicarboxylic aciduria mimicking long‐chain 3‐hydroxyacyl‐CoA dehydrogenase deficiency. J Inher Metab Dis 17:283–286. [DOI] [PubMed] [Google Scholar]
  • 11. Mayatepek E, Wanders RJA, Becker M, Bremer HJ, Hoffmann GF. 1995. Mitochondropathy presenting with non‐ketotic hypoglycaemia as 3‐hydroxydicarboxylic aciduria. J Inher Metab Dis 18:249–252. [DOI] [PubMed] [Google Scholar]

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