Abstract
We measured the activities of erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GPX) before therapy in 97 patients with cancer in various sites (gastrointestinal tract (GIT) (n=40), breast (n=30), and others (n=27)), and in 60 matched controls to assess antioxidant enzyme protection. Hemolysate hemoglobin (Hb) was measured spectrophotometrically. The activity of SOD (U/g Hb) was significantly lower in all sites (when all the cancer sites were considered as a group), GIT, breast, and other sites compared to the controls (P<0.0001, P<0.0001, P<0.0001 and P<0.0001, respectively). The activity of GPX (U/g Hb) was significantly decreased in all sites, GIT, and breast cancer sites than in the controls (P=0.024, P=0.033, and P=0.043, respectively). Age showed a weak negative correlation with enzyme activities in controls and patients. There was no significant association between SOD and GPX activities in either the controls or the patients. These results suggest that there may be a greater antioxidant burden for SOD than GPX in cancer, and that a weak association exists between the activities of the two enzymes in antioxidant protection. © 2002 Wiley‐Liss, Inc.
Keywords: superoxide dismutase, glutathione peroxidase, hemoglobin, breast cancer, gastrointestinal tract cancer, other cancer sites
REFERENCES
- 1. Halliwell B. How to characterize a biological antioxidant. Free Radic Res Commun 1990;9:1–32. [DOI] [PubMed] [Google Scholar]
- 2. Harris ED. Regulation of antioxidant enzymes. FASEB J 1992;6:2675–2683. [DOI] [PubMed] [Google Scholar]
- 3. Fridovich I. Superoxide dismutases. Annu Rev Biochem 1975;44:147–159. [DOI] [PubMed] [Google Scholar]
- 4. Pektau A, Copps TP, Kelly K. Superoxide dismutase within bovine erythrocyte membrane. Biochem Biophys Acta 1981;645:71–80. [DOI] [PubMed] [Google Scholar]
- 5. McCord JM, Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J Biol Chem 1969;244:6049–6055. [PubMed] [Google Scholar]
- 6. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione per‐oxidase. J Lab Clin Med 1967;70:158–169. [PubMed] [Google Scholar]
- 7. Carmagnol F, Sinet PM, Jerome H. Selenium‐dependent and non‐selenium‐dependent glutathione peroxidases in human tissue extracts. Biochem Biophys Acta 1983;759:49–57. [DOI] [PubMed] [Google Scholar]
- 8. Edwards CJ. Experiences with red cell enzyme assays. Comp Haematol Int 1993;3:33–39. [Google Scholar]
- 9. Abiaka C, Al‐Awadi F, Olusi S. Effect of prolonged storage on the activities of superoxide dismutase, glutathione reductase and glutathione peroxidase. Clin Chem 2000;46:566–567. [PubMed] [Google Scholar]
- 10. Misra HP, Fridovich I. The generation of superoxide during the autoxidation of haemoglobin. J Biol Chem 1972;647:6960–6966. [PubMed] [Google Scholar]
- 11. Cerutti PA. Oxy‐radicals and cancer. Lancet 1994;344:796–798. [DOI] [PubMed] [Google Scholar]
- 12. Ames BN, Cathcart R, Schwiers E, Hochstein P. Uric acid provides an antioxidant defence in humans against oxidant‐ and radical‐caused aging and cancer. Proc Natl Acad Sci U S A 1981;78:6858–6862. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13. Kumar K, Thangaraju M, Sachdanandam P. Changes observed in the antioxidant system of the blood of post‐menopausal women with breast cancer. Biochem Int 1991;25:371–380. [PubMed] [Google Scholar]
- 14. Bewick M, Coutie W, Tudhope GR. Superoxide dismutase, glutathione peroxidase and catalase in the red cells of patients with malignant lymphoma. Br J Haematol 1987;65:347–350. [DOI] [PubMed] [Google Scholar]
- 15. Pawlowicz Z, Zachara BA, Trafikowska U, Maciag A, Marchaluk E, Nowicki A. Blood selenium concentrations and glutathione peroxidase activities in patients with breast cancer and advanced gastrointestinal cancer. J Trace Elem Electrol Health Dis 1991;5:275–277. [PubMed] [Google Scholar]
- 16. Zachara BA, Marchaluk‐Wisniewska E, Maciag A, Peplinski J, Skokowski J, Lambrecht W. Decreased selenium concentration and glutathione peroxidase in blood and increase of tissue parameters in malignant tissue of lung cancer patients. Lung 1997;175:321–332. [DOI] [PubMed] [Google Scholar]
- 17. Robinson MF, Godfrey PJ, Thompson CD, Rea HM, Van Rij AM. Blood selenium and glutathione peroxidase activity in normal subjects and in surgical patients with and without cancer in New Zealand. Am J Clin Med 1979;32:1477–1485. [DOI] [PubMed] [Google Scholar]
- 18. Halliwell B, Gutteridge JMC. Protection against oxidants in biological systems: the superoxide theory of oxygen toxicity In: Halliwell B, Gutteridge JMC, editors. Free radicals in biology and medicine. 2nd ed. Oxford: Oxford University Press; 1989. p 86–114. [Google Scholar]
- 19. Heap L, Ward RJ, Abiaka CD, et al. The influence of acetaldehyde on oxidative status, dopamine metabolism and visual discrimination task. Biochem Pharmacol 1995;50:263–270. [DOI] [PubMed] [Google Scholar]