Abstract
The 384‐well microplate contains four times as many wells as the regular 96‐well microplate. Establishing enzyme linked immunosorbent assay (ELISA) on 384‐well microplate should lead to savings in reagents and specimens. To determine that ELISAs on 384‐well microplate have acceptable assay precision, ELISAs for tumor markers, including AFP, PSA‐ACT, CEA, CA 125, CA 15‐3, and CA 19‐9, were compared to the same ELISAs established on 96‐well microplate. We found that ELISAs established on 384‐well microplate had similar sensitivity and covered similar concentration ranges as ELISAs on 96‐well microplate. All within‐day and day‐to‐day precisions for the 384‐ELISA had %CV less than 10%. Compared to ELISA on 96‐well microplate, 384‐ELISA used less reagents, less specimen, and exhibited approximately a two‐fold increase in sensitivity. Overall cost of the 384‐384‐ELISA was also greatly reduced. Our results suggest that 384‐ELISA is suitable for use in routine clinical laboratories. J. Clin. Lab. Anal. 17:241–246, 2003. © 2003 Wiley‐Liss, Inc.
Keywords: tumor marker, ELISA, 384‐well microplate, 96‐well microplate
| Abbreviations | |
|---|---|
| AFP | alpha‐fetoprotein |
| Ab | antibody |
| CEA | carcino‐embrionic antigen |
| BSA‐PBA | 0.01 mol/L phosphate buffer at pH 7.2 containing 1% BSA |
| ELISA | enzyme linked immunosorbent assay |
| HRP | horse radish peroxidase |
| mAb | monoclonal antibody |
| PSA‐ACT | prostate specific antigen‐alpha1‐antichymotrypsin complex |
| PBS | phosphate buffered saline |
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