Abstract
The present work describes modification of a widely used salting‐out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β‐globin gene. The salting‐out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re‐extract the samples. J. Clin. Lab. Anal. 14:280–283, 2000. © 2000 Wiley‐Liss, Inc.
Keywords: DNA extraction, nucleated cells, salting‐out, phenol/chloroform, PCR
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