Skip to main content
PMC home page
Journal of Clinical Laboratory Analysis logoLink to Journal of Clinical Laboratory Analysis
. 2002 Nov 7;16(6):295–298. doi: 10.1002/jcla.10056

Pre‐ and postsurgical detection of IgG, IgM, and IgA specific to hydatidosis by ELISA with purified antigen enriched with the 5/B antigen complex

Olga Doiz 1, Rafael Benito 1,✉, Joaquina Gil 1, Almudena Rojas 2, M Carmen Rubio 1, Antonio Osuna 3
PMCID: PMC6808017  PMID: 12424802

Abstract

An enzyme‐linked immunoassay (ELISA) using purified 5/B Echinococcus enriched antigen was used to follow IgG, IgM, and IgA antibody levels pre‐ and posttreatment or surgical removal of hydatid cysts. The sensitivity was 97%, 37.5%, and 54.5%, respectively, and the specificity was 95.7%, 100%, and 98.9%, respectively. All isotypes could be detected 3 years after surgical removal of cysts in patients showing no remaining cyst evidence. This was especially true for IgG, which persisted in 85.2% of the patients. The data indicate that antigen purification improves specificity without affecting sensitivity, although this new antigen offers no advantages in the postsurgical monitoring of the patients. Clin. Lab. Anal. 16:295–298, 2002. © 2002 Wiley‐Liss, Inc.

Keywords: Echinococcus granulosus, hydatidosis, antigens, serological follow‐up

REFERENCES

  • 1. Tassi C, Baldelli F, Papili R, Valigi D. Specific IgM antibody response, evaluated by micro‐ELISA, to various antigen fractions from sheep hydatid fluid and scolices of E. granulosus, in sera from patients affected by hydatid disease, before and after surgery. Boll Ist Sieroter Milan 1984;63:140–144. [PubMed] [Google Scholar]
  • 2. Guisantes JA, Rubio M, Díaz R. Aplicación de un método inmunoenzimático (ELISA) al diagnóstico de la hidatidosis humana. Bol Sanit Panam 1981;90:160–168. [PubMed] [Google Scholar]
  • 3. Navarrete N, Jersic MI, Denis R. Comparación de tres técnicas en el diagnóstico serológico de la hidatidosis humana. Bol Chil Parasitol 1995;50:97–100. [PubMed] [Google Scholar]
  • 4. Njerhu FM, Gathuma JM, Okelo GBA, Tumboh‐Oeri AG. Diagnosis of human hydatid disease in surgically‐confirmed cases by use of the indirect haemagglutination test based on a thermo‐stable lipoprotein and on unfractionated hydatid cyst fluid. Am Trop Med Parasitol 1989;83:299–303. [DOI] [PubMed] [Google Scholar]
  • 5. Orduña A, Castrodeza J, Gobernado C, Rodríguez‐Torres A. Aplicación de un método immunoenzimático al diagnóstico serológico de la hidatidosis humana. II. Especificidad y sensibilidad de las pruebas ELISA IgG e IgM específicas. Laboratorio 1985;476:113–126. [Google Scholar]
  • 6. Schantz PM, Shanks D, Wilson M. Serologic cross‐reactions with sera from patients with echinococcosis and cysticercosis. Am J Trop Med Hyg 1980;29:609–612. [DOI] [PubMed] [Google Scholar]
  • 7. Vicente F, Guisantes JA. Diagnóstico de la hidatidosis humana. Nuestra experiencia con cinco métodos inmunológicos. Med Clin (Barc) 1984;82:93–96. [PubMed] [Google Scholar]
  • 8. Force L, Torres JM, Carrillo A, Buscá J. Evaluation of eight serological tests in the diagnosis of human echinococcosis and follow‐up. Clin Infect Dis 1992;15:473–480. [DOI] [PubMed] [Google Scholar]
  • 9. Varela‐Díaz VM, Coltorti EA, D'Alessandro A. Immunoelectrophoresis test showing Echinococcus granulosus arc 5 in human cases of Echinococcus vogeli and cysticercosis. Am J Trop Med Hyg 1978;27:554–557. [DOI] [PubMed] [Google Scholar]
  • 10. Verastegui M, Moro P, Guevara A, Rodríguez T, Miranda E, Gilman RH. Enzyme‐linked immunoelectrotransfer blot test for diagnosis of human hydatid disease. J Clin Microbiol 1992;30:1557–1561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11. Matossian RM, Radwan H, Meshefedjian GA. Immunoglobulin classes of reactive antibodies in diagnosis of human hydatidosis. Helminth 1991;28:41–47. [Google Scholar]
  • 12. Bouchara JP, Robert P, Senet JM, Leynia P, Jarrige R. Mise en évidence de communautés antigèniques hôte‐parasite dans l'hydatidose. Bull Soc Path Exot 1985;78:707–711. [PubMed] [Google Scholar]
  • 13. Pezzella M, Galli C, Delia S, Solice F, Vullo V, Zennaro F. Fractionation and characterization of hydatid fluid antigens with identification of an antigen similar to human serum albumin. Trans R Soc Trop Med Hyg 1984;71:821–826. [DOI] [PubMed] [Google Scholar]
  • 14. Cameron GL, Satveley JM. Blood group P substance in hydatid cyst fluids. Nature 1957;179:147–148. [DOI] [PubMed] [Google Scholar]
  • 15. Makni S, Ayed KH, Dalix AM, Oriol R. Immunological localization of blood pl antigen in tissues of Echinococcus granulosus . Ann Trop Med Parasitol 1992;86:87–88. [DOI] [PubMed] [Google Scholar]
  • 16. Russi S, Siracusano A, Vicari G. Isolation and characterization of a blood Pl active carbohydrate antigen of Echinococcus granulosus cyst membrane. J Immunol 1974;112:1061–1069. [PubMed] [Google Scholar]
  • 17. Sbihi Y, Janssen D, Osuna A. Serologic recognition of hydatid cyst antigens using different purification methods. Diagn Microbiol Infect Dis 1996;24:205–211. [DOI] [PubMed] [Google Scholar]
  • 18. Sbihi Y, Janssen D, Osuna A. Specific recognition of hydatid cyst antigens by serum IgG, IgE, and IgA using western‐blot. J Clin Lab Anal 1997;11:154–157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19. Rogan MT, Craig PS, Zeyhle E, Romig T, Lubano GM, Deshan L. Evaluation of a rapid dot‐ELISA as a field test for the diagnosis of cystic hydatid disease. Trans R Soc Trop Med Hyg 1991;85:773–777. [DOI] [PubMed] [Google Scholar]
  • 20. Oriol R, Williams JF, Pérez‐Esandi MV, Oriol C. Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. Am J Trop Med Hyg 1971;20:569–574. [DOI] [PubMed] [Google Scholar]
  • 21. Candolfi E, Kien T, Chaker E, Fourati M, Benyounes A. Intérêt de l'ántigénémie et des anticorps IgG, IgM, IgA et IgE dans l'immunologie du kyste hydatique. Résultats de l'immuno‐enzymologie. Bull Soc Path Ex 1985;78:700–706. [PubMed] [Google Scholar]
  • 22. Larrand L, Benito R, Sarría A, et al. Postoperative control for the persistence of positivity in three immunological tests used in the diagnosis of hydatidosis in children (E.L.I.S.A.‐specific IgE, indirect hemagglutination and E.L.I.S.A.‐total IgE). Allergol Immunopathol 1984;12:199–206. [PubMed] [Google Scholar]
  • 23. Orduña A, Espinosa M, Bratos R, Ortíz de Lejárazu R, Rodríguez Torres A. Estudio de la evolución serológica postquirúrgica en pacientes con hidatidosis mediante pruebas clásicas y pruebas de ELISA. Enferm Infecc Microbiol Clin 1986;4:213–220. [Google Scholar]
  • 24. Ravinder PT, Parija SC, Subba Rao KSVK. Evaluation of human hydatid disease before and after surgery and chemotherapy by demonstration of hydatid antigens and antibodies in serum. J Med Microbiol 1997;47:859–864. [DOI] [PubMed] [Google Scholar]
  • 25. Torres Rodríguez JM, Carrillo A, Casanovas M. Detección de anticuerpos IgE por un sistema ELISA de captura en la hidatidosis. Med Clin (Barc) 1989;92:37. [PubMed] [Google Scholar]

Articles from Journal of Clinical Laboratory Analysis are provided here courtesy of Wiley

RESOURCES