Abstract
To evaluate the feasibility of a synthetic polymer, sulphonated polyisoprene (SPIP), to be used as a new laboratory anticoagulant, hematological items were compared between blood samples anticoagulated with EDTA and SPIP, as were biochemical and electrolyte items between serum samples and SPIP‐anticoagulated blood. Among hematological items, the average platelet count of the SPIP group was significantly lower than that of the EDTA group, due to platelet aggregation in the SPIP group. Addition of kanamycin to SPIP, but not of aspirin or prostaglandin E1, restored platelet count to about 80% of that in the EDTA group, and increased coefficients of correlation with the EDTA group for most hematological items examined. For biochemical and electrolyte items, high (> 0.95) correlation coefficients were obtained for 15 of 18 items between the serum and SPIP‐anticoagulated samples. However, for some of these items, the addition of kanamycin decreased coefficients of correlation. Thus, SPIPs are excellent candidates for new anticoagulants that can be used for evaluation of hematological, biochemical, and electrolyte items with a single test tube in routine laboratory work. However, some improvements are required for use of kanamycin as an additive to inhibit platelet aggregation induced by SPIP, and in maintaining reliability of biochemical and electrolyte measurements. J. Clin. Lab. Anal. 14:180–187, 2000. © 2000 Wiley‐Liss, Inc.
Keywords: anticoagulant, sulphonated polyisoprene, synthetic polymer, kanamycin, platelet aggregation, laboratory testing
REFERENCES
- 1. Beugeling T, Van Der Does L, Bantjes A, Sederel WL. 1974. Antithrombin activity of a polyelectrolyte synthesized from cis‐1, 4‐polyisoprene. J Biomed Mater Res 8:375–379. [DOI] [PubMed] [Google Scholar]
- 2. Tamada Y, Murata M, Makino K, et al. 1998. Anticoagulant effects of sulphonated polyisoprenes. Biomaterials 19:745–750. [DOI] [PubMed] [Google Scholar]
- 3. Thomas C Bithell. 1993. Thrombosis and antithrombotic therapy In: Lee GR, Bithell TC, Foerster J, Athens JW, Lukens JN, editors. Wintrobe’s clinical hematology, 9th ed. Malvern, Pennsylvania: Lea and Febiger; p 1534. [Google Scholar]
- 4. Nitelius E, Brantmark B, Fredholm B, et al. 1984. Actions and interactions of acetylsalicylic acid, salicylic acid and diflunisal on platelet aggregation. Eur J Clin Pharmacol 27:165–168. [DOI] [PubMed] [Google Scholar]
- 5. Sils D, Rodgers SE, Lloyd JV, et al. 1988. Inhibition of platelet aggregation and thromboxane production by low concentrations of aspirin in vitro. Clin Sci 74:491–497. [DOI] [PubMed] [Google Scholar]
- 6. Bithell TC. 1993. The physiology of hemostasis In: Lee GR, Bithell TC, Foerster J, Athens JW, Lukens JN, editors. Wintrobe’s clinical hematology, 9th ed. Malvern, Pennsylvania: Lea and Febiger; p 545–546. [Google Scholar]
- 7. Zhang Z, Tatsumi N, Tsuda I, Iwashita K. 1999. Long‐term preservation of platelet count in blood for external quality control surveillance using Prostaglandin E1. Clin Lab Haematol 21:71. [DOI] [PubMed] [Google Scholar]
- 8. Sakurai S, Shiojima I, Tanigawa T, Nakahara K. 1997. Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA‐dependent pseudothrombocytopenia. Br J Haematol 99:817–823. [DOI] [PubMed] [Google Scholar]
- 9. Van Der Does L, Beugeling T, Froehling PE, Bantjes A. 1979. Synthetic polymers with anticoagulant activity. J Polym Sci Polym Symp 66:337–348. [Google Scholar]
- 10. Van Der Does L, Hofman J, Van Utteren TEC. 1973. Reaction of n‐chlorosulfonyl isocyanate with unsaturated polymers. Route to a synthesis of polyampholytes. J Polym Sci Polym Lett Edn 11:169–172. [Google Scholar]
- 11. Van Der Does L, Van Duiji JF, Sederel LC, Bantjes A. 1980. Modified polymers from cis‐1, 4‐polyisoprene. J Polym Sci Polym Lett Edn 18:53–58. [Google Scholar]