Abstract
Yeast forms of Paracoccidioides brasiliensis produce polydispersed high molecular mass (h‐MM) antigens. We investigated the antibodies to an h‐MM antigen from P. brasiliensis by immunoblotting and ELISA in sera from paracoccidioidomycosis (PCM) patients. IgG from the sera of chronic PCM patients was able to recognize the h‐MM antigen at a higher frequency in the cell‐free antigen (CFA) (8/13) than in the somatic antigen (SA) (2/13), as assessed by immunoblotting. The CFA was fractionated by Sephadex G‐200 chromatography, and fraction 17 (F17) with the h‐MM antigen of approximately 366 kDa was used in ELISA to analyze specific levels of IgG and IgE. Patients with the chronic form showed significantly higher levels of IgG (P<0.05) but not IgE (P>0.05) to F17 by ELISA, compared to patients with the acute form or to healthy donors. In conclusion, CFA is better than SA as a source of the P. brasiliensis h‐MM antigen. This study reveals a new characteristic to differentiate between the acute and chronic forms of PCM, by demonstrating a higher level of seric IgG to h‐MM antigen in chronic compared to acute PCM patients. J. Clin. Lab. Anal. 19:199–204, 2005. © 2005 Wiley‐Liss, Inc.
Keywords: Paracoccidioides brasiliensis, paracoccidioidomycosis, high molecular mass antigen, IgG, IgE
REFERENCES
- 1. San Blas G. Paracoccidioidomycosis and its etiological agent Paracoccidioides brasiliensis . J Med Vet Mycol 1993;31:99–113. [PubMed] [Google Scholar]
- 2. Coutinho ZF, Silva D, Lazéra M, et al. Paracoccidioidomycosis mortality in Brazil (1980–1995). Cad Saude Publica 2002;18:1441–1454. [DOI] [PubMed] [Google Scholar]
- 3. Franco M, Montenegro MR, Mendes RP, Marques SA, Dillon NL, Mota NG. Paracoccidioiodomycosis: a recently proposed classification of its clinical forms. Rev Soc Brasil Med Trop 1987;20:129–132. [DOI] [PubMed] [Google Scholar]
- 4. Brummer E, Castaneda E, Restrepo A. Paracoccidioidomycosis: an update. Clin Microbiol Rev 1993;2:89–117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5. Franco M. Host–parasite relationship in paracoccidioidomycosis. J Med Vet Mycol 1987;25:5–18. [DOI] [PubMed] [Google Scholar]
- 6. Bernard G, Neves CP, Gryschek RCB, Duarte AJS. Severe juvenile type paracoccidioidomycosis in an adult. J Med Vet Mycol 1995;33:67–71. [DOI] [PubMed] [Google Scholar]
- 7. McWeen JG, Bedoya V, Patino MM, Salazar ME, Restrepo A. Experimental murine paracoccidioidomycosis induced by inhalation of conidia. J Med Vet Mycol 1987;25:165–175. [DOI] [PubMed] [Google Scholar]
- 8. Saraiva EC, Altermani A, Franco MF, Unterkircher CS, Camargo ZP. Paracoccidioides brasiliensis‐gp43 used as paracoccidioidin. J Med Vet Mycol 1996;34:155–161. [DOI] [PubMed] [Google Scholar]
- 9. Botteon FAG, Camargo ZP, Benard G, Coelho RF, Chamone DAF, Itano EN. Paracoccidioides brasiliensis–reactive antibodies in Brazilian blood donors. Med Mycol 2002;40:387–391. [DOI] [PubMed] [Google Scholar]
- 10. Wanke B, Londero AT. Epidemiology and paracoccidioidomycosis infection In: Franco M, Lacaz CS, Restrepo‐Moreno A, Del‐Negro G, editors. Paracoccidioidomycosis. 1st ed. Boca Raton, FL: CRC Press; 1994. p 109–120. [Google Scholar]
- 11. Montenegro MR, Franco M. Pathology In: Franco M, Lacaz CS, Restrepo‐Moreno A, Del‐Negro G. editors. Paracoccidioidomycosis. 1st ed. Boca Raton, FL: CRC Press; 1994. p 67–86. [Google Scholar]
- 12. Mello LM, Silva‐Vergara ML, Rodrigues‐Junior V. Patients with active infection with Paracoccidioides brasiliensis present a Th2 immune response characterized by high interleukin‐4 and interleukin‐5 production. Hum Immunol 2002;63:149–154. [DOI] [PubMed] [Google Scholar]
- 13. Baida H, Biselli PJC, Juvenale M, et al. Differential antibody isotype expression to major Paracoccidioides brasiliensis antigen in juvenile and adult form paracoccidioidomycosis. Microbes Infect 1999;1:273–278. [DOI] [PubMed] [Google Scholar]
- 14. Arango M, Yarzábal L. T‐cell dysfunction and hyperimmunoglobulinemia E in paracoccidioidomycosis. Mycopathology 1982;79:115–124. [DOI] [PubMed] [Google Scholar]
- 15. Puccia R, Schenkman S, Gorin PAJ, Travassos LR. Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen. Infect Immunol 1986;53:199–206. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16. Biselli PJC, Juvenale M, Mendes‐Giannini MJS, Duarte AJS, Benard G. IgE antibody response to the main antigenic component of Paracoccidioides brasiliensis in patients with paracoccidioidomycosis. Med Mycol 2001;39:475–478. [DOI] [PubMed] [Google Scholar]
- 17. Mamoni RL, Rossi CL, Camargo ZP, Blotta MHSL. Capture enzyme‐linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis. Am J Trop Med Hyg 2001;65:237–241. [DOI] [PubMed] [Google Scholar]
- 18. Pier GB, Lyczak JB, Wetzler LM. Immunology, infection, and immunity. Washington, DC: ASM Press; 2004. [Google Scholar]
- 19. Yarzábal LA. Anticuerpos precipitantes especificos de la blastomicosis sudamericana revelados por inmunoelectroforesis. Rev Inst Méd Trop São Paulo 1971;13:320. [PubMed] [Google Scholar]
- 20. Camargo ZP, Taborda CP, Rodrigues EG, Travassos LR. The use of cell‐free antigens of Paracoccidioides brasiliensis in serological tests. J Med Vet Mycol 1991;29:31–38. [PubMed] [Google Scholar]
- 21. Travassos LR. Immunochemistry of Paracoccidioides brasiliensis antigens In: Franco M, Lacaz CS, Restrepo‐Moreno A, Del‐Negro G, editors. Paracoccidioidomycosis. 1st ed. Boca Raton, FL: CRC Press; 1994; p 67–86. [Google Scholar]
- 22. Graybill JR, Tollemar J, Torres‐Rodríguez JM, Walsh TJ, Roilides E, Farmaki E. Antifungal compounds: controversies, queries and conclusions. Med Mycol 2000;38(suppl I):323–333. [PubMed] [Google Scholar]
- 23. Polonelli L, Casadevall A, Han Y, et al. The efficacy of acquired humoral and cellular immunity in the prevention and therapy of experimental fungal infections. Med Mycol 2000;38:281–292. [PubMed] [Google Scholar]