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Kinetic parameters for M. extorquens La-, Ce-, and Nd-XoxF, determined using the XoxG/cyt c-linked activity assay at 30 °C (mean ± SD for 3 independent experiments).
| La-XoxF | Ce-XoxF | Nd-XoxF | |
|---|---|---|---|
| Vmax (nmol min−1 mg−1) | 570 ± 30 | 560 ± 10 | 610 ± 20 |
| Km, XoxG (μM) | 2.5 ± 0.1 | 4.4 ± 0.4 | 7.6 ± 1.0 |
| kcat (s−1) | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.3 ± 0.1 |
| kcat/Km (μM−1 s−1) | 0.49 ± 0.03 | 0.28 ± 0.03 | 0.17 ± 0.02 |
