Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Escherichia coli) | torS | NA | EcoCyc:G6514; UniProt:P39453 | |
| Strain, strain background (Escherichia virus HK022) | HK022 | PMID: 4569213 | RefSeq:NC_002166 | Dr. Max E. Gottesman (Columbia University) |
| Strain, strain background (E. coli) | DFE12 | this paper | MG1655 attBλ::(cat PtorCAD-yfp) ompA-cfp (HK022)n | |
| Strain, strain background (E. coli) | DFE34 | this paper | NRG 857C ΔlacIZY::PtorCAD-yfp-FRT-kan-FRT | |
| Strain, strain background (E. coli) | JNC151 | this paper | MG1655 (HK022)n | |
| Strain, strain background (E. coli) | JNC163 | PMID: 29502970 | MG1655 ΔlacZYA::FRT-cat-FRT torT-lacZ-FRT-kan-FRT ΔtorR | |
| Strain, strain background (E. coli) | JNC166 | PMID: 29502970 | MG1655 ΔlacZYA::FRT torS-lacZ-FRT-kan-FRT | |
| Strain, strain background (E. coli) |
JNC168 | this paper | MG1655 ΔlacZYA::FRT-cat-FRT (HK022)n torT-lacZ-FRT-kan-FRT ΔtorR | |
| Strain, strain background (E. coli) | JNC169 | this paper | MG1655 ΔlacZYA::FRT torS-lacZ-FRT-kan-FRT (HK022)n | |
| Strain, strain background (E. coli) | JNC173 | this paper | MG1655 ΔfhuA::FRT-kan-FRT attBλ::(cat PtorCAD-yfp) ompA-cfp (HK022)n | |
| Strain, strain background (E. coli) | JNC174 | this paper | MG1655 ΔfhuA::FRT-kan-FRT attBλ::(cat PtorCAD-yfp) ΔxylAFG::PtetA-mcherry-FRT | |
| Strain, strain background (E. coli) | JNC175 | this paper | MG1655 ΔlacZYA::FRT torS-lacZ-FRT-kan-FRT (HK022)n attLHK022::Ω | |
| Strain, strain background (E. coli) | MG1655 | Coli Genetic Stock Center | CGSC:7740; RefSeq:NC_000913 | |
| Strain, strain background (E. coli) | MMR8 | PMID: 25825431 | MG1655 attBλ::(cat PtorCAD-yfp) ompA-cfp | |
| Strain, strain background (E. coli) | NRG 857C | PMID: 21108814 | RefSeq:NC_017634 | Dr. Alfredo G. Torres (UTMB) |
| Strain, strain background (E. coli) | PK13196 | this paper | MG1655 lacZ::kan-PtorS-(GTG)lacZ ΔiscR::FRT | |
| Strain, strain background (E. coli) | PK13199 | this paper | MG1655 lacZ::kan-PtorS-(ATG)lacZ ΔiscR::FRT | |
| Recombinant DNA reagent | pDSW206 | PMID: 9882665 | ori(pBR322) lacIq
amp Ptrc attenuated promoter. Dr. Jon Beckwith (Harvard University) |
|
| Recombinant DNA reagent | pMR26 | PMID: 25825431 | pDSW206 torT | |
| Recombinant DNA reagent | pPK7179 | PMID: 15659690 | ori(pBR322) ter(spf) amp RNA-1 | |
| Recombinant DNA reagent | pPK12669 | this paper | pPK7179 with −152 to +28 bp relative to the torS ATG start codon from MG1655 in XhoI/BamHI sites |
|
| Recombinant DNA reagent | pPK13256 | this paper | pPK7179 with −231 to +28 bp relative to the torS ATG start codon from JNC151 in XhoI/BamHI sites | |
| Sequence-based reagent | native torS | this paper | 32P-labeled DNA oligonucleotide: 5’-TTAACAGCGCCATCAG-3’ | |
| Sequence-based reagent | HK022/torS | this paper | 32P-labeled DNA oligonucleotide: 5’-GGGTCAGGGTTAAATTCACGG-3’ | |
| Peptide, recombinant protein | E. coli σ70 RNA polymerase holoenzyme | New England Biolabs | NEB:M0551S | |
| Commercial assay or kit | HiSpeed Plasmid Maxi Kit | Qiagen | Qiagen:12662 | |
| Commercial assay or kit | MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit | Lucigen | Lucigen:MM070150 | |
| Commercial assay or kit | Sequenase Version 2.0 DNA Sequencing Kit | USB | USB:70770 | |
| Software, algorithm | BLAST | PMID: 23609542 | RRID:SCR_004870 | |
| Software, algorithm | ClermonTyping | PMID: 29916797 | v. 1.4.0 | |
| Software, algorithm | ggridges | Comprehensive R Archive Network | RRID:SCR_003005 | v. 0.5.0 |
| Software, algorithm | Mauve | PMID: 20593022 | RRID:SCR_012852 | v. 2015-02-25 |
| Software, algorithm | MUSCLE | PMID: 15034147 | v. 3.8.1551 | |
| Software, algorithm | R | R Foundation for Statistical Computing | RRID:SCR_001905 | v. 3.4.4 |
| Software, algorithm | SnapGene | GSL Biotech | RRID:SCR_015052 | v. 5.0b3 |