Figure 4.

Metabolic phonotype of IR-780^H fibroblasts. (A) Up-regulated signaling pathways in IR-780^H fibroblasts compared to control fibroblasts. Real time (B) OCR, (C) ECAR and (D) mean ratios of ECAR:OCR of IR-780^H and control fibroblasts in mitochondrial stress testing.**,P<0.01. (E) Real time ECAR of IR-780^H and control fibroblasts in glycolysis stress testing. (F) Intracellular and (G) extracellular lactate levels in IR-780^H and control fibroblasts. (H) Realtime RT-PCR and (I) Western blot analysis of glycolytic enzyme and HIF-1α expression in IR-780^H and control fibroblasts. Testing the effects of glycolytic inhibitors (2-DG,6-AN,3PO) (J) and BSP (K) on the uptake of IR-780 in IR-780^H and control fibroblasts. (L) Up-regulated gene expression of members in solute carrier family in IR-780^H fibroblasts compared to control fibroblasts. Results of Western blot (M) and Real time PCR (N) of SLCO2A1 and SLCO1B3 in IR-780^H and control fibroblasts. (O) Testing the effects of siRNA of SLCO2A1 and SLCO1B3 on the uptake of IR-780 in fibroblasts. (P) The expression of LDHA, LDHB, LDHC, HIF-1αand SLCO2A1 and uptake of IR-780 (Q) in fibroblasts cultured in hypoxia ( 5% O2) with or without LW6.