Figure 6.

The adhesive ability of V. splendidus to coelomocytes demonstrated by fluorescence (a-c) and colony counting (b). The green fluorescence was the labeled Vs-DTAF and the red fluorescence was the labeled coelomocyte. (a), the cells blocked by DLD1 antibody; (b), the cells blocked by DLD2 antibody; (c), the control group. (d), Colony counting of the cells that adhered to coelomocytes. After adhesion and washes, the resuspesion cell solution was 1.0 × 10³-fold dilution, and 50 μL was spread on 2216E plate. Data were means of three independent experiments, and are presented as means ± SD. *P< 0.05, **P< 0.01.