Table 2.
HCP and DNA content in a CHO cell culture supernatant and eluted fractions after purification of trastuzumab on columns with ZCaTetraCys, MabSelect SuRe and MabSelect PrismA, including the log reduction values (LRVs). ZCaTetraCys demonstrates comparable HCP LRVs to MabSelect SuRe and MabSelect PrismA and a sufficient DNA LRV.
| Sample HCP | Elution HCP | LRV | Sample DNA | Elution DNA | LRV | |
|---|---|---|---|---|---|---|
| (ng/mg mAb) | (ng/mg mAb) | HCP | (pg/mg mAb) | (pg/mg mAb) | DNA | |
| ZCaTetraCys | 7.2 * 102 | 2.3 | 1.2 * 103 | 3.2 | ||
| MabSelect SuRe | 1.6 * 105 | 5.2 * 102 | 2.5 | 1.9 * 106 | 81 | 4.4 |
| MabSelect PrismA | 5.9 * 102 | 2.4 | 4.0 | 5.7 |