Figure 3.

Introduction of the ‘knobs-into-holes’ mutations in the CH3 domains affects the assembly of MoAbs and DuoMabs. Modifications that favor heterodimerization (e.g., “knobs-into-holes” mutations) will increase generation of MoAbs. (a) SEC and (b) SDS-PAGE reveals preferential MoAb assembly (1 = DuoMab, 2 = MoAb). The shown SEC profiles reflect the chromatogram of a preparative purification (in order to show all species without pooling just the desired and show them). Due to the fact that some molecules express better than others (different binders), we obtained different yields after affinity chromatography. The height and retention times differ due to the use of different SEC columns according to the amount of protein.