Correction to: Type I Diabetes Delays Perfusion and Engraftment of 3D Constructs by Impinging on Angiogenesis; Which can be Rescued by Hepatocyte Growth Factor Supplementation

Correction to: Cellular and Molecular Bioengineering 10.1007/s12195-019-00574-3

The values on the Y-axis for Figs. 1a, 2a, and 5a were in ng/L, not in molar. To meet the CAMB journal requirements, we have converted the values to molar and updated the graphs.

Corrected Figs. 1a, 2a, and 5a are shown below.

Figure 1.

HGF is secreted by adipose-derived microvessel fragments in 3D constructs and mediates angiogenic sprouting. (a) In vitro analysis of HGF secretion from pre-vascularized engineered constructs. Pre-vascularized engineered constructs were maintained in 20% FBS-DMEM. Media was collected at the indicated time-points and HGF secretion was measured by ELISA (mean ± SEM; one-way ANOVA, Bonferroni correction, p ≤ 0.0001 vs. day 0). (b) Blockage of HGF/c-MET significantly reduces sprouting angiogenesis. Pre-vascularized constructs were maintained in 20% FBS-DMEM containing either anti-HGF antibody (a-HGF), c-MET inhibitor (SU11274), IgG control, or DMSO (vehicle) for 7 days (mean ± SEM; n = 3 one-way ANOVA, Bonferroni correction, anti-HGF p ≤ 0.05, SU11274, p ≤ 0.0001).

Figure 2.

HGF is secreted from cultured perivascular cells. (a) Quantification of HGF secretion from smooth muscle cells or pericytes by ELISA (mean ± SEM; two-way ANOVA, Bonferroni correction; p ≤ 0.0001). (b) HGF expression by perivascular cells was confirmed in vitro in the pre-vascularized engineered constructs. Immunofluorescence staining of HGF at day 5 shows that HGF (red) co-localizes with alpha-smooth muscle cell actin (aSMA, perivascular cell marker, green) but not lectin (endothelial cell marker, blue).

Figure 5.

Diabetes impairs HGF secretion and decreases angiogenic sprouting in pre-vascularized engineered constructs. (a) In vitro analysis of HGF secretion from microvessel-based engineered constructs under high glucose conditions. Pre-vascularized tissues containing 20,000 microvessel fragments/ml were maintained in culture for 5 days in DMEM containing 10% FBS with either 25 mM d-glucose or 25 mM mannitol (control). HGF secretion was quantified by ELISA of culture supernatants (mean ± SEM; n = 3; two-way ANOVA, Bonferroni correction, *p = 0.3, **p = 0.0003). (b) Culture in high glucose (25 mM d-glucose) media significantly inhibits sprouting as compared to control (25 mM mannitol) and low glucose (5 mM d-glucose). Data were coded to: 0 = no sprouts; 1 = sprouting; 2 = high sprouting (mean ± SEM; n = 3; one-way ANOVA, Bonferroni correction, *p = 0.01 vs. 25 mM d-mannitol or 5 mM d-glucose).