Structural and antitumoral characteristic dataset of the chitosan based magnetic nanocomposite

Hamed Tashakkorian; Vahid Hasantabar; Monire Golpour

doi:10.1016/j.dib.2019.104583

. 2019 Sep 28;27:104583. doi: 10.1016/j.dib.2019.104583

Structural and antitumoral characteristic dataset of the chitosan based magnetic nanocomposite

Hamed Tashakkorian ^a,^b,^∗, Vahid Hasantabar ^c, Monire Golpour ^d

PMCID: PMC6817675 PMID: 31673586

Abstract

The evaluation on the characteristic dataset and figures presented here, are related to our latest research data entitled “Fabrication of chitosan based magnetic nanocomposite by click reaction strategy; evaluation of nanometric and Cytotoxic characteristics” [1]. FTIR, Vibrating Sample Magnetometer (VSM) measurements, Xray diffraction (XRD) information and the resulted figures for structural confirmation of the prepared chitosan based nanocomposite are presented in this article. The morphological changes of the Fibroblast, Saos, MCF7 and Hela cell lines after treatment with the mention compound were displayed. The additional adsorption data for the synthesized nanobiocomposite were also demonstrated with graphs.

Keywords: Magnetic nanobiocomposite, Click chemistry, Antitumoral assay, Structural data, Cell lines

Specifications Table

Subject area	Chemistry, Biology
More specific subject area	Preparation of chitosan based nanobiocomposite
Type of data	raw data, graph, figure
How data was acquired	The outcomes were provided by IR, VSM, XRD and MTT assay. Also some descriptions about the composite preparation and images of the morphology of cell lines were presented.
Data format	Raw, analyzed
Experimental factors	FTIR of the prepared samples, VSM and XRD of the composite, also the images of cell lines were apprised.
Experimental features	The nanocomposite was prepared and characterized using FTIR and imaged by SEM and TEM technique. Then thermophysical experiments were performed using DSC and TGA protocols. Biological characteristics were evaluated with MTT assay and the morphological effects were imaged by microscopic technique.
Data source location	Babol university of medical sciences, Mazandaran, Iran
Data accessibility	Available in this article
Related research article	Fabrication of chitosan based magnetic nanocomposite by click reaction strategy; evaluation of nanometric and Cytotoxic characteristics [1]

Open in a new tab

Value of the Data

•
This data presents the structural and physical characteristics of the synthesized biocomposite, from which researchers who are interested in preparation of novel chitosan based nanocomposite especially in medical field can take advantage of it.
•
The isotherm linear absolute and isotherm pressure composition plots data which is introduced as tables and figure can gain the attention of the chemical and environmental engineers for production of the new class of bioadsorbents.
•
The biocharacteristics of the prepared composite toward Fibroblast, MCF7, Hela, and Saos cell lines were investigated and the data can encourage researches towards assessments against other cancer cell typs.

Open in a new tab

1. Data

IR spectra were recorded on a Perkin-Elmer FT-IR-1710 spectrophotometer with the samples in KBr pellets. Fig. 1 displays the FT-IR spectra of the prepared compounds and the characteristic peaks data were introduced as Table 1. Vibrating Sample Magnetometer (VSM) measurements were performed by using a vibrating sample magnetometer (LDJ Electronics Inc., Model 9600) and the data was inserted as Table 2 and the resulted pattern was displayed in Fig. 2. The X-ray powder diffraction (XRD) of the catalyst was carried out on a Philips PW 1830 X-ray diffractometer with CuKα source (λ = 1.5418 Å) in a range of Bragg's angles (5–80°) at room temperature and demonstrated in Fig. 3. The crystal planes of Fe₃O₄ which confirm the existence of magnetic nanoparticles in the composite were assigned in Table 3 Brunauer–Emmett–Teller (BET) analysis were performed using automatic sorption analyzer ASAP 2020, Micromeritics, USA. The Isotherm Linear Absolute Plot and isotherm Pressure Composition dataset were inserted in Table 4, Table 5 respectively and the adsorption and desorption graphs were displayed in Fig. 4. The morphology of cancer cells after treatment with different concentrations of nanocomposite were displayed in Fig. 5.

Table 1.

FT-IR analysis data of a) Azidated chitosan, b) Chitosan-silane composite, c) Chitosan-silane clicked @ Fe₃O₄.

FT-IR Analysis	Wavenumber (cm⁻¹)
Azidated chitosan (a)	910, 1090, 1160, 1270, 1395, 1540, 1625, 2100, 2895, 3430 (br)
Chitosan-silane composite (b)	1070, 1150, 1420, 1650, 2945, 3265, 3450
Chitosan-silane clicked @ Fe₃O₄(C)	570, 1018, 1107, 1400, 1620, 2925, 3400

Open in a new tab

Table 2.

The detailed magnetization data versus applied field of MNC.

(Oe)	emu/g	(Oe)	emu/g	(Oe)	emu/g	(Oe)	emu/g	(Oe)	emu/g
1.90	−0.02	5501.11	24.62	321.11	13.21	−828.88	−18.44	−2469.45	−22.66
19.409	1.34	6001.11	24.78	236.11	11.40	−1057.49	−19.57	−1935.21	−21.89
33.72	2.44	6501.11	24.93	191.83	10.15	−1285.17	−20.37	−1545.17	−21.11
47.11	3.44	7001.11	25.07	157.97	9.11	−1545.17	−21.07	−1287.70	−20.42
62.11	4.46	7501.11	25.18	131.71	8.20	−1951.52	−21.87	−1051.93	−19.60
78.38	5.50	8001.11	25.29	111.58	7.39	−2469.45	−22.64	−828.88	−18.47
91.90	6.29	8501.11	25.40	91.11	6.49	−2998.88	−23.23	−743.88	−17.92
111.96	7.26	8001.11	25.29	78.04	5.74	−3498.88	−23.63	−658.88	−17.29
133.18	8.12	7501.11	25.18	61.75	4.71	−3998.88	−23.94	−573.88	−16.53
158.35	9.00	7001.11	25.06	45.95	3.68	−4498.88	−24.20	−488.88	−15.63
192.15	10.07	6501.11	24.92	32.08	2.70	−4998.88	−24.42	−403.88	−14.50
236.11	11.32	6001.11	24.81	17.14	1.56	−5498.88	−24.62	−318.88	−13.08
321.11	13.13	5501.11	24.63	0.50	0.20	−5998.88	−24.79	−239.66	−11.38
406.11	14.54	5001.11	24.44	−11.57	−0.72	−6498.88	−24.94	−175.97	−9.58
491.11	15.66	4501.11	24.22	−27.47	−1.96	−6998.88	−25.06	−140.75	−8.38
576.11	16.54	4001.11	23.96	−42.01	−3.01	−7498.88	−25.19	−113.56	−7.28
661.11	17.31	3501.11	23.65	−57.33	−4.00	−7998.88	−25.30	−91.45	−6.23
746.11	17.92	3001.11	23.26	−73.00	−4.95	−8498.88	−25.40	−72.20	−5.17
831.11	18.47	2501.11	22.75	−91.45	−6.01	−7998.88	−25.29	−57.41	−4.27
916.11	18.94	2001.11	22.07	−113.56	−7.04	−7498.88	−25.19	−40.59	−3.19
1178.04	20.00	1521.03	21.08	−141.48	−8.19	−6998.88	−25.06	−26.32	−2.21
1533.99	21.08	1173.47	20.03	−176.25	−9.47	−6498.88	−24.93	−11.81	−1.08
2001.11	22.04	916.11	18.97	−238.94	−11.30	−5998.88	−24.78	1.90	−0.02
2501.11	22.72	831.11	18.50	−318.88	−13.02	−5498.88	−24.63
3001.11	23.23	746.11	17.97	−403.88	−14.41	−4998.88	−24.43
3501.11	23.62	661.11	17.35	−488.88	−15.62	−4498.88	−24.20
4001.11	23.94	576.11	16.60	−573.88	−16.47	−3998.88	−23.94
4501.11	24.20	491.11	15.71	−658.88	−17.23	−3498.88	−23.63
5001.11	24.42	406.11	14.60	−743.88	−17.896	−2998.88	−23.25

Open in a new tab

Fig. 2 — Magnetization curves of Fe₃O₄@functionalized chitosan nanobiocomposite.

Fig. 3 — XRD patterns of the synthesized SiO₂/functionalized chitosan composite.

Table 3.

Data resulted from XRD pattern of MNC.

	Crystal planes (2-Theta)
Fe₃O₄	2 2 0 (30.4°)		3 1 1 (35.7°)			4 0 0 (43.4°)		4 2 2 (53.6°)			5 1 1 (57°)	4 4 0 (63.0°)
Chitosan/SiO₂	Broad Peak (15–30°)
Unassigned	12.2	18		21.3	24.8		25.4		28	76

Open in a new tab

Table 4.

Quantity of MNC-adsorption and desorption versus absolute pressure.

No.	MNC-Adsorption		MNC-Desorption
No.	Quantity (cm³/g STP)	Absolute Pressure (kPa)	Quantity (cm³/g STP)	Absolute Pressure (kPa)
1	0.0155	3.9491	3.1219	10.2207
2	0.0308	4.5614	6.2336	11.3728
3	0.0533	5.0750	11.0299	12.4181
4	0.0775	5.4451	15.8141	13.1678
5	0.1012	5.7218	20.6030	13.8167
6	0.1165	5.8714	30.1617	15.0107
7	0.5385	7.7433	37.9474	16.0667
8	0.9008	8.4642	47.5964	17.7131
9	1.1723	8.8480	57.5541	20.1034
10	2.8142	10.1713	68.1428	24.9174
11	4.5636	10.9367	76.8254	34.0477
12	6.3321	11.4933	87.8111	75.9624
13	8.1536	11.9367	90.7318	79.2349
14	9.9705	12.3060	95.2962	82.0726
15	14.1124	13.0277	97.0361	83.6500
16	18.3035	13.6566	99.0819	86.1214
17	22.4814	14.2307
18	30.8083	15.3401
19	39.1600	16.5298
20	47.4831	17.9148
21	51.6722	18.7439
22	59.9826	20.7978
23	68.2312	23.8461
24	72.5367	26.2876
25	80.4362	33.3906
26	89.6708	54.5202
27	94.0723	73.1871
28	97.0785	80.9530
29	99.0819	86.1214

Open in a new tab

Table 5.

Quantity of absolute pressure versus weight %N₂.

No.	MNC-Adsorption		MNC-Desorption
No.	Weight % N₂	Absolute Pressure (kPa)	Weight % N₂	Absolute Pressure (kPa)
1	0.0352	0.0155	0.0912	3.1219
2	0.0407	0.0308	0.1014	6.2336
3	0.0452	0.0533	0.1108	11.0299
4	0.0485	0.0775	0.1174	15.8141
5	0.0510	0.1012	0.1232	20.6030
6	0.0523	0.1165	0.1339	30.1617
7	0.0690	0.5385	0.1433	37.9474
8	0.0755	0.9008	0.1580	47.5964
9	0.0789	1.1723	0.1793	57.5541
10	0.0907	2.8142	0.2223	68.1428
11	0.0975	4.5636	0.3038	76.8254
12	0.1025	6.3321	0.6778	87.8111
13	0.1065	8.1536	0.7070	90.7318
14	0.1098	9.9705	0.7323	95.2962
15	0.1162	14.1124	0.7464	97.0362
16	0.1218	18.3035	0.7684	99.0819
17	0.1269	22.4814
18	0.1368	30.8083
19	0.1474	39.1600
20	0.1598	47.4831
21	0.1672	51.6722
22	0.1855	59.9826
23	0.2127	68.2312
24	0.2345	72.5367
25	0.2979	80.4362
26	0.4864	89.6708
27	0.6530	94.0723
28	0.7223	97.0785
29	0.7684	99.0819

Open in a new tab

Fig. 4 — (a) Isotherm linear absolute plot (b) isotherm pressure composition.

Fig. 5 — Morphology of the mentioned cell lines after incorporating the prepared nanobiocomposite samples in concentrations of 15.62, 31.5, 62.5 and 125 μg/mL.

2. Experimental design, materials and methods

The magnetic nanocomposite was prepared using chitosan. To prepare functionalized chitosan, the chitosan was azidated using chloroacetyl chloride and sodium azide. Then click reaction which has been incorporated in our recent studies [2,3] and also employed in some biological researches [4,5] was performed between functionalized chitosan and trimethoxy(3-(prop-2-yn-1-ylthio)propyl)silane. Then magnetization was done using ferric and ferrous chloride solution. The characteristic peaks for azidated at around 2100 cm⁻¹, C–H bond of triazole rings and Si–O–Si bonds at 3265 cm⁻¹ and 1150 cm⁻¹ respectively. The resulted FT-IR spectra and the corresponding data of the synthesized products were presented in Fig. 1 and Table 1. Also, the detailed FT-IR data including the transmittances at each wavenumbers for the compounds a, b, and c were provided as a supplementary file.

Magnetization experiments of the prepared magnetic nanocomposite (MNC) were obtained using VSM technique at room temperature. As can be seen in Fig. 2, this product with saturation magnetization value (M_s) of 25.4 (emu/g) has super paramagnetic characteristics. Moreover; the corresponding data were presented in Table 2.

The XRD pattern of the synthesized chitosan nanocomposite (Fig. 3) demonstrated the crosslinking reaction between Si groups and chitosan with the broad peak at 15–30°. Moreover the existence of magnetic nanoparticles in the structure was confirmed by determining the crystal planes of Fe₃O₄ nanoparticles (Table 3).

To attain adsorption data of the synthesized nanocomposite for further experiments, the samples were outgassed at 60 °C and then experiments according to the Brunauer–Emmett–Teller (BET) theory were performed. The isotherm plots were used to calculate the specific surface area and the average pore diameter of the chitosan/magnetic nanocomposite and the difference between adsorption and desorption steps.

For evaluating the cell cytotoxicity of the prepared sample (MNC) according to the literature [6], some known cell lines were considered including Fibroblast, MCF7, Hela, and Saos. The resulted data were surveyed in the main article and the morphology of the cell lines with treatment of different concentrations of the samples were imaged by microscopic technique and presented here in Fig. 5.

Acknowledgements

The authors gratefully acknowledge the support of the Babol University of Medical Sciences as well as Mazandaran University.

Footnotes

^{Appendix A}

Supplementary data to this article can be found online at https://doi.org/10.1016/j.dib.2019.104583.

Conflict of Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Appendix A. Supplementary data

The following is the Supplementary data to this article:

Multimedia component 1

mmc1.xlsx^{(110.4KB, xlsx)}

References

1.Hasantabar V., Tashakkorian H., Golpour M. Click reaction as a well-organized strategy for synthesis of chitosan based magnetic nanobiocomposite; appraisal of nanometric, thermophysical and antitumoral characteristics. Carbohydr. Polym. 2019;224:115163. doi: 10.1016/j.carbpol.2019.115163. [DOI] [PubMed] [Google Scholar]
2.Fallah Z., Nasr Isfahani H., Tajbakhsh M., Tashakkorian H., Amouei A. TiO2-grafted cellulose via click reaction: an efficient heavy metal ions bioadsorbent from aqueous solutions. Cellulose. 2018;25:639–660. [Google Scholar]
3.Hasantabar V., Lakouraj M.M., Tashakkorian H., Rouhi M. Designed Monomers and Polymers; 2016. Novel Nanocomposite Based on Poly(xanthoneamide-Triazole-Ethercalix) and TiO2 Nanoparticles: Preparation, Haracterization, and Investigation of Nanocomposite Capability in Removal of Cationic Water Pollutants; pp. 607–618. [Google Scholar]
4.Hou W., Luo Z., Zhang G., Cao D., Li D., Ruan H., Ruan B.H., Su L., Xu H. Click chemistry-based synthesis and anticancer activity evaluation of novel C-14 1,2,3-triazole dehydroabietic acid hybrids. Eur. J. Med. Chem. 2017;138:1042–1052. doi: 10.1016/j.ejmech.2017.07.049. [DOI] [PubMed] [Google Scholar]
5.Ma N., Wang Y., Zhao B.X., Ye W.C., Jiang S. The application of click chemistry in the synthesis of agents with anticancer activity. Drug Des. Dev. Ther. 2015;9:1585–1599. doi: 10.2147/DDDT.S56038. [DOI] [PMC free article] [PubMed] [Google Scholar]
6.Akbarian M., Mahjoub S., Elahi S.M., Zabihi E., Tashakkorian H. Urtica dioica L. extracts as a green catalyst for the biosynthesis of zinc oxide nanoparticles: characterization and cytotoxic effects on fibroblast and MCF-7 cell lines. New J. Chem. 2018;42:5822–5833. [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Multimedia component 1

mmc1.xlsx^{(110.4KB, xlsx)}

[bib1] 1.Hasantabar V., Tashakkorian H., Golpour M. Click reaction as a well-organized strategy for synthesis of chitosan based magnetic nanobiocomposite; appraisal of nanometric, thermophysical and antitumoral characteristics. Carbohydr. Polym. 2019;224:115163. doi: 10.1016/j.carbpol.2019.115163. [DOI] [PubMed] [Google Scholar]

[bib2] 2.Fallah Z., Nasr Isfahani H., Tajbakhsh M., Tashakkorian H., Amouei A. TiO2-grafted cellulose via click reaction: an efficient heavy metal ions bioadsorbent from aqueous solutions. Cellulose. 2018;25:639–660. [Google Scholar]

[bib3] 3.Hasantabar V., Lakouraj M.M., Tashakkorian H., Rouhi M. Designed Monomers and Polymers; 2016. Novel Nanocomposite Based on Poly(xanthoneamide-Triazole-Ethercalix) and TiO2 Nanoparticles: Preparation, Haracterization, and Investigation of Nanocomposite Capability in Removal of Cationic Water Pollutants; pp. 607–618. [Google Scholar]

[bib4] 4.Hou W., Luo Z., Zhang G., Cao D., Li D., Ruan H., Ruan B.H., Su L., Xu H. Click chemistry-based synthesis and anticancer activity evaluation of novel C-14 1,2,3-triazole dehydroabietic acid hybrids. Eur. J. Med. Chem. 2017;138:1042–1052. doi: 10.1016/j.ejmech.2017.07.049. [DOI] [PubMed] [Google Scholar]

[bib5] 5.Ma N., Wang Y., Zhao B.X., Ye W.C., Jiang S. The application of click chemistry in the synthesis of agents with anticancer activity. Drug Des. Dev. Ther. 2015;9:1585–1599. doi: 10.2147/DDDT.S56038. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib6] 6.Akbarian M., Mahjoub S., Elahi S.M., Zabihi E., Tashakkorian H. Urtica dioica L. extracts as a green catalyst for the biosynthesis of zinc oxide nanoparticles: characterization and cytotoxic effects on fibroblast and MCF-7 cell lines. New J. Chem. 2018;42:5822–5833. [Google Scholar]

PERMALINK

Structural and antitumoral characteristic dataset of the chitosan based magnetic nanocomposite

Hamed Tashakkorian

Vahid Hasantabar

Monire Golpour

Abstract

1. Data