Skip to main content
. 2019 Oct 28;8(11):63. doi: 10.1038/s41389-019-0172-9

Fig. 4. Ectopic PR55α expression in human normal pancreatic ductal cells (HPNE) activates YAP and inhibits the MOB1-LATS1/2 cascade.

Fig. 4

a Dox-inducible retroviral vector expressing PR55α (pRevTRE-PR55α) was constructed and retrovirus was produced as described in the “Materials and methods“ section. Subsequently, HPNE cells were transduced with both pRevTet-On (Clontech) that expresses rtTA and pRevTRE-PR55α (or pRevTRE-Control) and selected for resistant cells to both G418 (400 µg/ml) and Hygromycin B (200 µg/ml). Diagram demonstrates the Tet-inducible retroviral vector (pRevTRE-PR55α) expressing PR55α. b The transduced cells were induced for ectopic PR55α expression by incubation with increasing doses of Dox (1 µg/ml) for 3 days and the effect of PR55α on the phosphorylation and level of YAP and LATS1/2 analyzed by immunoblotting. GAPDH served as an internal control. c Control- and PR55α-transduced HPNE cells were incubated with Dox (1 µg/ml) for the indicated days and analyzed for the effect of PR55α on YAP and the Hippo pathway (MST1/2, MOB1, and LATS1/2), by analyzing their phosphorylation and levels by immunoblotting. GAPDH served as an internal control