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. 2019 Oct 29;9:15796. doi: 10.1038/s41598-019-49873-9

Publisher Correction: Development of a viability digital PCR protocol for the selective detection and quantification of live Erwinia amylovora cells in cankers

Ricardo D Santander 1, Christopher L Meredith 1, Srđan G Aćimović 1,
PMCID: PMC6817877  PMID: 31659171

Correction to: Scientific Reports 10.1038/s41598-019-47976-x, published online 08 August 2019

In the original version of the Article, the full error bars were omitted from Figure 9A due a technical error during publication.

In addition, in the Results and Discussion Section under the subheading ‘Direct transfer of a qPCR protocol to the QS3D dPCR system’,

“A known qPCR protocol for E. amylovora31 was transferred to the QS3D dPCR platform, using the same primer and probe concentrations and thermal cycling conditions as a first step to test the QS3D dPCR technology.”

now reads:

“A known qPCR protocol for E. amylovora31 was transferred to the QS3D dPCR platform, using the same primer and probe concentrations and thermal cycling conditions as a first step to test the QS3D dPCR technology.”

Finally, in the Legend of Table 2,

cLog Copies mL−1 = Log [copies μL−1rxn × 1/Drxn tube x1/EDNA Extr. X1/VolPl.Macerates]; i.e. Log (copies μL−1rxn × 1.79 × 103) for Apple, and Log (copies μL−1rxn × 4.81 × 103) for pear.”

now reads:

cLog Copies mL−1 = Log [copies μL−1rxn × 1/Drxn tube × 1/EDNA Extr × 1/VolPl.Macerates]; i.e. Log (copies μL−1rxn × 1.79 × 103) for Apple, and Log (copies μL−1rxn × 4.81 × 103) for pear.”

This has been corrected in both the PDF and HTML versions of the Article.


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