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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Hepatology. 2019 Jun 21;70(5):1714–1731. doi: 10.1002/hep.30700

Figure 5. YAP interacts with β-catenin and regulates its transcription activity in MSC-mediated immune regulation.

Figure 5

Bone marrow-derived macrophages (BMMs, 1×106) were co-cultured with MSCs (2×105) for 24h followed by LPS (100 ng/ml) stimulation. (A) and (B) Immunofluorescence staining of nuclear YAP (green) and β-catenin (red) in macrophages after co-culture with or without MSCs. DAPI was used to visualize nuclei (blue). Scale bars, 20μm. (C) Immunoblot-assisted analysis of cytosol and nuclear YAP and β-catenin in macrophages after co-culture with or without MSCs. Representative of three experiments. (D) Immunofluorescence staining for macrophage YAP (green) and β-catenin (red) co-localization in the nucleus after co-culture with MSCs. DAPI was used to visualize nuclei (blue). Scale bars, 10μm. (E) Immunoprecipitation analysis of YAP and β-catenin in macrophages after co-culture with MSCs. Representative of three experiments. (F) BMMs were co-transfected with 1µg β-catenin-luc and CRISPR YAP activation vectors. The luciferase activity was measured after 48h (n=3–4 samples/group). Data represent the mean±SD. *p<0.05.