Figure 4.

In the Early Stage, Injury Induces More Robust Wnt and Proliferation Response in the Soft Palate

(A–B) On PSD3, representative Masson trichrome staining showing the healing front in (A) the hard palate and (B) the soft palate.

(C-F) In Axin2^LacZ/+ mice, Xgal staining was performed to detect Wnt-responsive cells in (C) the healing hard palate on PSD3 and (D) the intact hard palate. The orange box area in (C) has been enlarged in (C′). Xgal staining was performed to detect Wnt-responsive cells in (E) the healing soft palate on PSD3 and (F) the intact soft palate. The orange box area in (E) has been enlarged in (E′).

(G-I) EdU staining was used to examine cell proliferation in (G) the healing hard palate on PSD3 and (H) the intact hard palate. The orange box area in (G) has been enlarged in (G′). EdU staining was used to examine cell proliferation in (I) the healing soft palate on PSD3 and (J) the intact soft palate. The orange box area in (I) has been enlarged in (I′).

(K) Quantification of EdU^+ve cells (n = 6, 5 sections of each mouse). Abbreviation: lp, lamia proper; in, injury; PSD, post-surgery day; hp, hard palate; sp, soft palate. Scale bars: 100 μm.

Data represent mean ± SD. See also Figure S4.