Figure 6.

Wnt-Responsive Population Enlarges in Response to Proliferation but Prunes after Homeostasis Is Reestablished

(A) A schematic diagram showing the lineage tracing strategy. Mice were injected with one dose of tamoxifen at the age of 3 months. Two days later, a 2-mm full-thickness injury was generated in the hard and soft palates. Wnt-responsive cells were analyzed 3D, 7D, 10D, 14D, and 21D after surgery.

(B-C) On PSD3, GFP^+ve Wnt-responsive cells were examined in the healing (B) hard palate and (C) soft palate.

(D-E) On PSD7, GFP^+ve Wnt-responsive cells were examined in the healed (D) hard palate and (E) soft palate.

(F) On PSD14, abundant GFP^+ve Wnt-responsive cells still existed in the healed hard palate.

(G) By PSD14, GFP^+ve Wnt-responsive cells were returned to normal level.

(H) On PSD10, TUNEL were co-stained with GFP^+ve Wnt-responsive cells in the soft palate.

(I) EdU staining showing the proliferation of cells in both hard and soft palates on PSD14. Orange bracket indicates the injury site.

(J) On PSD21, TUNEL were co-stained with GFP^+ve Wnt-responsive cells in the hard palate.

(K) GFP^+ve Wnt-responsive cells in the hard palate 28D after injury. Wnt-responsive cells (green) were co-immunostained with β4 integrin (β4, red, basement membrane). Yellow arrows indicate Wnt-responsive cells in suprabasal layers. White arrowhead indicates Wnt-responsive cells in basal layer. Abbreviation: lp, lamia proper; hp, hard palate; sp, soft palate; PSD, post-surgery day. Scale bars: 100 μm.

See also Figures S5–S9.