Table 1.
A. HF target panel genes differentially expressed in TGF-β treated fibroblasts and their exosomes (N=3 in each group). Genes that were overlapped in both fibroblasts and exosomes and were differentially expressed in TGF-β treated compared to control, up-regulated (bold) and down-regulated (italic) and opposite expression level (bold-italic). B. Genes that were differentially expressed in fibroblasts treated with TGF-β compared to control but not in exosomes and that were differentially expressed in exosomes but not in the cells.
| A | |||||
|---|---|---|---|---|---|
| No. | Gene ID | Fibroblasts |
Exosomes |
||
| logFC | PValue | logFC | PValue | ||
| Genes up-regulated in both | |||||
| 1 | ACTC1 | 3.62579036366492 | 1.15E-06 | 4.34306220386333 | 3.14E-134 |
| 2 | ADRB1 | 4.15067746467641 | 0.00045106525616 | 3.58662983153525 | 0.000102019938012 |
| 3 | COL1A1 | 2.47423904492034 | 0.000570546020783 | 0.743907933075704 | 0.020136359996399 |
| 4 | LDB3 | 2.9632124860499 | 3.23E-05 | 1.38185448721437 | 6.58E-21 |
| 5 | TPM1 | 4.10551345735947 | 6.03E-07 | 0.770209933262394 | 0.012942373820731 |
| Genes down-regulated in both | |||||
| 6 | KCND2 | -2.74506316216588 | 0.001077509731735 | -2.12756529627957 | 6.13E-08 |
| 7 | MYOZ2 | -3.35999843083144 | 2.62E-06 | -2.98880023680181 | 1.51E-65 |
| Genes with opposite expression level | |||||
| 8 | ACTN2 | 6.06578448673155 | 3.96E-06 | -0.801875420811882 | 8.58E-05 |
| 9 | NPPA | 4.25915757536294 | 0.005639664808369 | -4.21802918591052 | 1.53E-06 |
| B | ||||||
|---|---|---|---|---|---|---|
| Fibroblasts |
No. | Exosomes |
||||
| Gene ID | logFC | PValue | Gene ID | logFC | PValue | |
| CRY2 | 1.6726351782109 | 8.36E-03 | 1 | ABCC9 | -1.09572406425757 | 2.17E-14 |
| JUN | 2.78417683699569 | 4.84E-03 | 2 | ALPL | -1.41077509253921 | 1.50E-18 |
| KCNIP2 | 7.03609527206253 | 5.48E-08 | 3 | CCL2 | -1.14833682425684 | 4.24E-13 |
| MYH6 | 3.77116708556277 | 4.07E-03 | 4 | CENPQ | -0.615372155168008 | 3.12E-04 |
| NFKB2 | 1.69138565530869 | 7.75E-03 | 5 | CETP | -0.890917086984853 | 1.85E-03 |
| PABPC1L | 2.64596377213132 | 1.19E-03 | 6 | CLOCK | -0.35650277263649 | 1.05E-02 |
| PRPF38B | 3.27560062275906 | 7.55E-05 | 7 | DMD | -0.683525966384984 | 9.39E-05 |
| RFXAP | 3.12742667953124 | 5.99E-04 | 8 | FPGT | -0.703648294320673 | 2.19E-05 |
| TAZ | 2.45853295533953 | 1.06E-03 | 9 | HK2 | -0.400356916424286 | 4.78E-03 |
| TNNT2 | 3.38181281033613 | 2.80E-05 | 10 | IL1B | -1.32651139448865 | 1.81E-16 |
| VCL | 1.91838305242001 | 3.01E-03 | 11 | IL8 | -1.37742335988644 | 5.85E-20 |
| VEGFA | 2.70240761679433 | 6.72E-05 | 12 | IRF2 | -1.15106670302639 | 1.90E-14 |
| 13 | NFKB1 | -0.63849139111603 | 1.50E-04 | |||
| 14 | NPPB | -0.712579596363141 | 3.28E-07 | |||
| 15 | PDK4 | -2.20859122918407 | 1.96E-02 | |||
| 16 | PER2 | -0.699122984280214 | 5.00E-05 | |||
| 17 | PER3 | -0.964767500776701 | 4.00E-10 | |||
| 18 | PIK3R1 | -0.751483395402562 | 2.59E-06 | |||
| 19 | PPM1D | -0.632871440676927 | 4.14E-04 | |||
| 20 | PRKAG2 | -0.732622316470305 | 5.31E-08 | |||
| 21 | RAP2B | -0.572958972427159 | 1.36E-03 | |||
| 22 | RPS7 | -0.387174853351925 | 1.46E-02 | |||
| 23 | SLC9B2 | -0.601755533570746 | 8.14E-06 | |||
| 24 | SMAD3 | -0.827924411520658 | 4.98E-08 | |||
| 25 | SOAT1 | -0.595371811931464 | 9.46E-06 | |||
| 26 | TBX2 | -0.327192758172327 | 2.16E-02 | |||
| 27 | VTN | -1.59149277204745 | 6.40E-12 | |||
| 28 | WEE1 | -1.26720980348616 | 3.28E-15 | |||
| 29 | XPNPEP3 | -0.484427749366068 | 3.80E-04 | |||
| 30 | ATRNL1 | 0.548910996272464 | 1.09E-04 | |||
| 31 | DSP | 0.680847783454209 | 5.57E-05 | |||
| 32 | EDN1 | 0.595509958339416 | 9.18E-05 | |||
| 33 | ERF | 0.355272517151082 | 7.75E-03 | |||
| 34 | GLS | 0.503383673453361 | 2.82E-03 | |||
| 35 | IL6 | 1.17304888700213 | 2.12E-10 | |||
| 36 | JUP | 1.01001172541033 | 2.05E-11 | |||
| 37 | MYL2 | 2.81250831532571 | 1.31E-04 | |||
| 38 | MYLK | 0.455152258426705 | 7.85E-03 | |||
| 39 | NPAS2 | 0.553593186395444 | 1.33E-03 | |||
| 40 | PDIA6 | 0.432633677021006 | 6.79E-03 | |||
| 41 | ZNF704 | 0.661279719253398 | 2.07E-05 | |||