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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Structure. 2019 Aug 8;27(10):1485–1496.e4. doi: 10.1016/j.str.2019.07.012

Figure 4.

Figure 4.

iASPP and ASPP2 enhance the activity of PP-1c towards pNPP and inhibit PP-1cα towards Phosphorylase a. (A) PP-1cα colorimetric assay using para-nitrophenylphosphate (pNPP) as a substrate for PP-1cα. PP-1cα was incubated alone or in the presence of iASPP608–828, ASPP2905–1128, bovine serum albumin (BSA) or Inhibitor-2. Graph depicts the total amount of pNPP substrate left in each sample at each time point. Error bars indicate s.d of 3 replicates. (B) PP-1c colorimetric pNPP assay comparing the activity of PP-1cα WT or PP-1cα1-300 alone, or in the presence of WT iASPP608–828, or iASPP608–828 L625A mutant. Error bars indicate s.d of 3 replicates.