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. 2019 Oct 16;8(10):1262. doi: 10.3390/cells8101262

Figure 3.

Figure 3

Fasudil blocks Th17 differentiation. (A) Naïve CD4+ T cells pooled from 8 mice were stimulated with anti-CD3/CD28 for two days without or with fasudil (0–50 µM). Interleukin (IL)-17 and IL-21 in the culture supernatants were determined by ELISA. (B,D) Naïve CD4+ T cells were differentiated under Th17/Treg conditions for four days and restimulated with PMA plus ionomycin for five hours in the presence of vehicle (PBS) or fasudil (30 µM) throughout the culture. Cells were collected for IL-17 intracellular staining. Percentages of IL-17+ CD4+ T cells are shown (B) in representative dot plots and (C) summarized in a bar graph. (D) IL-17, IL-17F, and IL-21 in the culture supernatants were determined by ELISA. Data (mean + SD of triplicates) are representative of two independent experiments. ** p < 0.01 compared with PBS-treated groups.