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. 2019 Oct 28;8:e49493. doi: 10.7554/eLife.49493

Key resources table.

Reagent
type (species)
or resource
Designation Source or
reference
Identifiers Additional information
Genetic reagent (M. musculus) Ednrb flox/flox The university of Edinburgh, Bagnall et al., 2006; Ge et al., 2006
Genetic reagent (M. musculus) Pdgfra-cre Jackson labs #013148
Genetic reagent (D. rerio) Rse Tlf802 Frohnhöfer et al., 2013; Krauss et al., 2014
Genetic reagent (D. rerio) Tg(mbp:EGFP) The University of Edinburgh,
Almeida et al., 2011
Antibody Mouse monoclonal anti CNPase Atlas AMAb91072 1:2000
Antibody Rat monoclonal anti MBP Serotec MCA409S 1:250
Antibody Rat monoclonal anti PECAM1 BD Pharmingen 550274 1:100
Antibody Rabbit monoclonal anti S100β Thermo MA5-12969 1:100
Antibody Rabbit monoclonal anti IBA1 Abcam ab178846 1:500
Antibody Rabbit polyclonal anti Laminin Abcam ab11575 1:300
Antibody Rabbit polyclonal anti OLIG2 Millipore ab9610 1:100
Antibody Mouse monoclonal anti CC1 Abcam ab16794 1:300
Antibody Mouse monoclonal anti GAPDH Millipore MAB374 1:1000
Antibody Mouse monoclonal anti Beta actin Abcam ab 8226 1:1000
Antibody Rabbit polyclonal anti, Phospho-PKCε S729 Abcam 88241 1:1000
Commercial assay, kit Phospho-explorer antibody array Full Moon Biosystems, Phospho-explorer array (PEX100)
Sequence-based reagent Edn1 Advanced Cell Diagnostics 435221
Sequence-based reagent Edn2 Advanced Cell Diagnostics 418221
Sequence-based reagent Edn3 Advanced Cell Diagnostics Custom made
Sequence-based reagent Pecam1 Advanced Cell Diagnostics 316721-C3
Sequence-based reagent Olig2 Advanced Cell Diagnostics 447091
Sequence-based reagent Ednrb Advanced Cell Diagnostics 473801
Peptide, recombinant protein BQ3020 Tocris 1189 100 ng/mL
Chemical compound, drug BQ788 Tocris 1500 100 ng/mL
Chemical compound, drug FR236924 Tocris 0373 25 µM
Other Microfibers The Electrospinning company 1–2 mcro diameter poly-l-lactic acid
Software, algorithm Any-maze software http://www.anymaze.co.uk/
Software, algorithm ImageJ https://imagej.nih.gov/ij/
Software, algorithm Graphpad Prism https://www.graphpad.com/scientific-software/prism/