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. 2019 Sep 25;9(24):7268–7281. doi: 10.7150/thno.33006

Figure 3.

Figure 3

Effect of Maf1 upregulation on AB-induced hypertrophy. The experiments were all carried out in mice. (A-B) Western blots showing Maf1 and vinculin expression in mouse hearts, and the quantitative analysis of those blots. Vinculin was used as an internal control; n=3. (C) Images of whole hearts from Maf1-overexpressing mice 4 weeks after sham or AB surgery. (D-E) The heart or lung/body weight ratio was detected and showed that upregulation of Maf1 expression could attenuate AB-induced hypertrophy and pulmonary edema; Ad-GFP+Sham n=7; Ad-Maf1+Sham n=7; Ad-GFP +AB n=6; Ad-Maf1+AB n=5. (F-J) The effects of Maf1 upregulation on echocardiographic parameters (EF, LVEDd, LVEDs and LVPWd). (J) The effect of Maf1 upregulation on ANP mRNA expression was determined by q-PCR, and GAPDH was used as an internal control; n=4-5. (K-L) Images of H&E-stained heart sections from mice at 4 weeks after sham or AB surgery; n=3. *p<0.05 versus the corresponding control group. **p<0.01 versus the corresponding control group.