FIG 1.
Retro-orbital and tail vein injections produce equivalent outcomes. BALB/c mice were infected with 1.25 × 106 spores of M. circinelloides f. circinelloides strain 1006PhL through either the retro-orbital or tail vein route. There were five mice in each group. (A) The two methods of infection produce equivalent mortality. (B) The retro-orbital method of infection produces equivalent homogenized fungal burdens in randomly sampled moribund mice (N = 2). (C) Histopathological analysis of brains from mice infected with spores by retro-orbital and tail vein injection, stained with GMS (Gomori methenamine-silver) reveals abundant fungal elements from both routes of infection.