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. 2019 Oct 10;24(20):3648. doi: 10.3390/molecules24203648

Figure 6.

Figure 6

The effect of heat incubation on the DFF yield of co-immobilized tri-enzymes (■), separately immobilized tri-enzymes (▲) and free tri-enzymes (●). Reaction conditions: 35 mg of co-immobilized tri-enzymes or 105 mg of separately immobilized tri-enzymes (containing 35 mg of immobilized galactose oxidase, 35 mg of immobilized catalase and 35 mg of immobilized horseradish peroxidase) or free tri-enzymes (20 U of galactose oxidase, 8000 U of catalase and 500 U of horseradish peroxidase); 200 mM of HMF; Tris-HCl buffer (50 mM, pH 6.6) for co-immobilized tri-enzymes and separately immobilized tri-enzymes, and Tris-HCl buffer (50 mM, pH 7.1) for free tri-enzymes; at 37 °C.