Table 3.
Primers and probes used for real-time qPCR.
| Name | Species | Amplicon Size (bp) | Sequence (5′-3′) | Reference |
|---|---|---|---|---|
| END37-F | Erwinia virus Ea214 | 149 | TTCAGCTTTAGCGGCTTCGAGA | This study |
| END37-R | AGCAAGCCCTTGAGGTAATGGA | |||
| END37-P | /56-ROXN/AGTCGGTACACCTGCAACGTCAAGAT/3IAbRQSp/ | |||
| STS3-F | Erwinia virus Era103 | 96 | GACAAACAAGAACGCGGCAACTGA | [64] |
| STS3-R | ATACCCAGCAAGGCGTCAACCTTA | |||
| STS3-P | /56-FAM/AGATGAAGTAGGTTATCTTCACAGTGCCCT/3BHQ_1/ | |||
| N14-F | Erwinia virus Ea9-2 | 168 | CATTGGGTAATCCCTTTGAG | This study |
| N14-R | GATAGACTGGTTCCCCTGTG | |||
| N14-P | /56-FAM/TCTGGTGGA/ZEN/CAGAGACGATGTAAT/3IABkFQ/ | |||
| RDH311-F | Erwinia virus Ea35-70 | 183 | TGGAAGGTCTTCTTCGAGAC | This study |
| RDH311-R | GACTACCTGGGGATGTTCAG | |||
| RDH311-P | /56-ROXN/GACGGAAAAGATCACGGTACTCTT/3IAbRQSp/ | |||
| Ea-Lsc-F | E. amylovora | 105 | CGCTAACAGCAGATCGCA | [65] |
| Ea-Lsc-R | AAATACGCGCACGACCAT | |||
| Ea-Lsc-P | /5Cy5/CTGATAATCCGCAATTCCAGGATG/3IAbRQsp/ | |||
| Pa-Gnd-F | P. agglomerans | 73 | TGGATGAAGCAGCGAACA | [65] |
| Pa-Gnd-R | GACAGAGGTTCGCCGAGA | |||
| Pa-Gnd-P | /5HEX/AAATGGACCAGCCAGAGCTCACTG/3BHQ_1/ |