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Redox Report : Communications in Free Radical Research logoLink to Redox Report : Communications in Free Radical Research
. 2016 Jan 29;21(5):209–218. doi: 10.1080/13510002.2015.1101889

Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidity

Joanna Robaczewska 1,2,✉, Kornelia Kędziora-Kornatowska 1, Robert Kucharski 3, Maria Nowak 3, Marta Muszalik 1, Maciej Kornatowski 4, Józef Kędziora 5
PMCID: PMC6837343  PMID: 26824276

Abstract

Background: There is strong evidence that hypertension and depression are comorbid and oxidative stress is implicated in both pathologies. We aimed to elucidate the relationship between biochemical markers of the antioxidant-pro-oxidant equilibrium and depression in hypertension.

Methods: Blood was collected from patients diagnosed with depression, hypertension, or comorbid depression and hypertension and healthy age- and sex-matched controls. Whole blood reduced glutathione, erythrocyte superoxide dismutase (SOD-1), glutathione peroxidase (GPx-1), glutathione reductase (GR), malondialdehyde (MDA), and plasma hydrogen peroxide (H2O2) were assayed using spectrophotometry, and heme oxygenase (HO-1) levels were determined immunoenzymatically.

Results: Both hypertension and depression were associated with altered antioxidant-pro-oxidant profiles. Decreased GPx-1 and SOD-1 activities, increased GR activity, increased levels of GSH, and increased concentrations of MDA and H2O2 were observed in patients compared to controls. Inducible HO-1 was specifically decreased in patients with depression and was significantly associated with both the prevalence and severity of depressive symptoms.

Conclusions: Heme oxygenase is a biological factor that might explain the relationship between inflammation, oxidative stress, and the biological and functional changes in brain activity in depression. HO-1 is a candidate depression biomarker and provides an avenue for novel preventative and diagnostic strategies against this disease.

Keywords: Depression, Hypertension, Oxidative stress, Heme oxygenase, Antioxidant enzymes

Highlights

  • Hypertension, depression, and comorbidity with both diseases were associated with increased peroxidation and antioxidant markers.

  • Inducible heme oxygenase (HO-1) was a specific marker of the antioxidant-pro-oxidant equilibrium in depressed patients.

  • HO-1 levels were decreased in patients with depression and negatively correlated with depressive symptoms.

  • Decreased expression of HO-1 may contribute to the risk of depression in hypertension.

Introduction

Epidemiological studies have shown that hypertensive patients are at an increased risk of depression. This relationship is bidirectional; a number of cross-sectional and cohort studies have shown that depressed patients are also more likely to develop cardiovascular diseases than the general population.1–3 Studies on the biological factors underlying depression and somatic health have resulted in several plausible mechanistic explanations based on metabolic, immunological, autonomic, and hypothalamic–pituitary–adrenal axis dysregulation, which are recognized biological causes of clinical depression.4 However, there is a lack of specific biomarkers associated with depression, not least due to the large clinical heterogeneity in depressed patients. This has hindered efforts to identify the biological, genetical, and environmental causes of depression, and it is crucial that depressive subtypes and comorbidities are taken into account in studies of biological correlates of depression.

The pathogenesis of depression and essential hypertension is multifactorial. Of the biological mechanisms thought to contribute to hypertension and depression, oxidative stress pathways have been shown to play a role in cardiovascular and neurological function. Specifically, reactive species such as superoxide (O2•−) and peroxynitrite (ONOO2212) affect vascular tone and are excessively produced in hypertension. Peroxynitrite is a reactive nitrogen species formed via radical–radical coupling reactions between O2•− and nitric oxide (NO), which react rapidly in the vascular wall. As a result, the relationship between increased ROS production and high blood pressure can be explained by enhanced inactivation of the vasodilator NO by O2•−.5–6 Extensive lipid peroxidation, overproduction of endothelin, proliferation of vascular smooth muscle cells, and increased renal sodium reabsorption in hypertension have also been attributed to oxidative stress.5

Depression is also a complex multifactorial disease that involves numerous biological processes and pathways including inflammation, oxidative stress, and progressive neuropathological defects. Neural, endocrine, immune, and metabolic pathways are implicated in major depressive disorder and are comprehensively reviewed elsewhere.7–9 A relationship between oxidative and nitrosative stress and depression is supported by evidence of increased free radical levels, lowered antioxidant levels, and increased lipid peroxidation in depressed patients.10–12

Oxidative stress results from the increased production of free radicals, decreased antioxidant levels, diminished activities of antioxidant enzymes, and impaired repair of oxidative damage.13 The response to oxidative stress also includes induction of some protective mechanisms, and heme oxygenases (HOs) are widely considered central components of the mammalian stress response that protect against oxidative stress.14

Since oxidative stress is common to the etiopathology of depression and hypertension, we hypothesized that an antioxidant-pro-oxidant imbalance contributes to the high susceptibility of hypertensive patients to depression and that biomarkers of the pro- and antioxidant equilibrium are associated with the prevalence and severity of depression in hypertensive patients. Here, we aimed to elucidate the relationship between biomarkers of peroxidation and antioxidant defense in depression and hypertension.

Methods

Subjects

Four groups of patients aged 50 or above were recruited 15 patients diagnosed and currently treated for depression (recurrent depressive disorder); 20 patients diagnosed and currently treated for hypertension; 16 patients diagnosed and currently treated for hypertension with symptoms of comorbid depression; and 19 healthy age- and sex-matched controls. Subjects were recruited from the Psychoneurology of the Elderly Center Sue Ryder Home and the Department and Clinic of Geriatrics, Collegium Medicum UMK (Bydgoszcz, Poland). The 21-item Beck Depression Inventory (BDI) with a cut–off >1315 was used to assess the severity of depressive symptoms a week prior to study enrollment.

Patients with stage one and two hypertension were included in the study according to Polish Society of Hypertension guidelines (2011). Hypertension stages were defined by the values of systolic blood pressure (SBP) and diastolic blood pressure (DBP): stage one was when the SBP and DBP values were 140–159 and 90–99 mmHg, respectively, and stage two was when the SBP and DBP values were 160–179 and 100–109 mmHg, respectively.

All subjects were evaluated by standard physical examination and routine clinical laboratory tests. Exclusion criteria were mental disorders coded on DSM axes I and II, diabetes, endocrine disorders, hepatitis, cancer, chronic infection, hematologic malignancies, hemolytic anemia, a diagnosed vitamin B12 or folic acid deficiency, and patients on oral iron therapy.

Written informed consent was obtained from all study participants. The Nicolaus Copernicus University in Torun Human Ethics Committee approved the study protocol and the study was conducted under the tenets of the Declaration of Helsinki.

Blood sample collection and biochemical analyses

Venous heparin and cloth-activated fasting blood samples were collected in the morning (08:00 am). Reduced glutathione (GSH) concentrations were determined spectrophotometrically in whole blood samples by the Beutler method (1975) using 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB). GSH reacts non-enzymatically with DTNB to generate GSSG and the highly colored 5-thio-2-nitrobenzoic acid (peak absorbance at 420 nm). The remaining blood was centrifuged and, after a careful removal of plasma and the buffy coat, red blood cells were washed with cold isotonic saline (0.89% NaCl, pH = 7.4). The sediment was then lysed by the addition of distilled water to obtain red blood cell hemolysate by three freeze-thaw cycles. Glutathione peroxidase (GPx-1), glutathione reductase (GR), and superoxide dismutase (SOD-1) activities were determined in erythrocytes from hemolyzed blood samples according to Paglia and Valentine16, Flohe and Gunzler17, and Misra and Fridovich18, respectively.

SOD-1 activity was determined in ethanol/chloroform-treated samples by measuring the inhibition of autoxidation of adrenaline at pH 10.2, 37°C. GR activity was determined by measuring the oxidation of NADPH via the decrease in absorbance at 340 nm at 37°C in the presence of GSSG. GPx activity was determined by measuring the oxidation of NADPH at 340 nm at 25°C in the coupled reaction with t-butylhydroperoxide, GSH, and GR. Hemolyzed erythrocyte samples were also assayed for malondialdehyde (MDA) using the 2-thiobarbituric acid method read at 532 nm.

Serum samples were aliquoted and immediately stored at −80°C for further analysis of heme oxygenase (HO-1). Fresh plasma samples were analyzed for hydrogen peroxide (H2O2) concentration using the PeroxiDetect Kit (Sigma) according to the manufacturer's instructions; sample absorbance was measured at 560 nm, normalized, and expressed as nmol/ml. HO-1 was assayed using a human enzyme-linked immunosorbent assay (ELISA; USCN Life Science Inc., Cloud-Clone Corp., Houston, USA) kit. The mean optical density of duplicate samples was analyzed in the same run by the same operator. The average coefficient of variation between duplicates was 4.93.

Statistical analyses

Multiple comparisons between groups were conducted using non-parametric statistics, since the sample sizes of the groups were not large enough to test for the assumption for normality.

The Kruskal–Wallis non-parametric ANOVA was used for multiple comparisons. Linear regression was used to assess the relationship between biochemical markers of pro-antioxidant equilibrium and severity of depressive symptoms expressed as BDI scores. The relationship between biochemical markers and the prevalence of depressive symptoms was analyzed by logistic regression. Clinical and biochemical characteristics of patients and controls did not show significant confounding effects in regression models. Statistica version 9 software was used for statistical analyses. The results were considered significant if the two-sided P-value was 0.05 or less.

Results

Antioxidant-pro-oxidant equilibrium in patient groups and controls

There were significant differences in the antioxidant-pro-oxidant equilibrium in all groups of patients. Multiple comparisons revealed significant differences in parameter values between patients and controls but not between different patient groups. The concentration of H2O2 was significantly increased in all patients groups compared to controls (P < 0.001; Fig. 1). Likewise, there were significantly increased levels of the lipid peroxidation marker MDA in patients with hypertension, depression, and hypertension with comorbid depression compared to controls (P < 0.001; Fig. 2). Concentrations of GSH were significantly increased in hypertensive and depressed patients (P < 0.01; Fig. 3).

Figure 1.

Figure 1

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Concentration of H2O2 in plasma of patients with depression (n = 15), hypertension (n = 20), and hypertension with comorbid depression (n = 16) compared with controls (n = 19); *P < 0.001.

Figure 2.

Figure 2

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Concentration of MDA in erythrocytes of patients with depression (n = 15), hypertension (n = 20), and hypertension with comorbid depression (n = 16) compared with controls (n = 19); *P < 0.001.

Figure 3.

Figure 3

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Concentration of GSH in whole blood of patients with depression (n = 15), hypertension (n = 20), and hypertension with comorbid depression (n = 16) compared with controls (n = 19); *P < 0.01.

With respect to antioxidant enzyme activities, GR activity was significantly increased in patients with hypertension and patients with depression (P < 0.001) compared to controls (Fig. 4), while GPx activity was significantly lower in hypertensive, depressive, and comorbid patients compared to controls (P < 0.001; Fig. 5). The activity of SOD-1 in hypertensive patients and depressed patients was significantly lower compared to healthy subjects (P < 0.001; Fig. 6), while lower levels of HOs were seen in the plasma of patients with depression and patients with comorbid depression and hypertension (P < 0.05; Fig. 7).

Figure 4.

Figure 4

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Activity of GR in erythrocytes of patients with depression (n = 15), hypertension (n = 20), and hypertension with comorbid depression (n = 16) compared with controls (n = 19); *P < 0.01.

Figure 5.

Figure 5

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Activity of GPx-1 in erythrocytes of patients with depression (n = 15), hypertension (n = 20), and hypertension with comorbid depression (n = 16) compared with controls (n = 19); *P < 0.001.

Figure 6.

Figure 6

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Activity of SOD-1 in erythrocytes of patients with depression (n = 15), hypertension (n = 20), and hypertension with comorbid depression (n = 16) compared with controls (n = 19); *P < 0.001.

Figure 7.

Figure 7

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Concentration of HO-1 in serum of patients with depression (n = 15), hypertension (n = 20) and hypertension with comorbid depression (n = 16) compared with controls (n = 19); *P < 0.005.

The relationship between antioxidant-pro-oxidant equilibrium and depression

We next assessed the relationship between biochemical markers of the antioxidant-pro-oxidant equilibrium and the severity of depression symptoms expressed by BDI scores. There was a significant negative relationship between BDI scores and HO-1 levels; decreased enzyme expression was correlated with increased severity of depressive symptoms in depressed subjects (P < 0.05) (described by BDI = 17.0259–14.6551*HO1; see Fig. 8).

Figure 8.

Figure 8

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Scatter plot of BDI against HO-1 in study population. BDI – Beck Depression Inventory; HO-1 – heme oxygenase.

The relationship between HO and the prevalence of depressive symptoms was confirmed by logistic regression, which allowed classification of hypertensive patients as depressed based on HO concentrations significantly better than by chance alone (P < 0.01).

Discussion

Here, we show that there are significant disturbances in the antioxidant-pro-oxidant equilibrium in patients with hypertension and depression. Both diseases were associated with increased levels of peroxidation and altered antioxidant enzyme activities. Interestingly, the antioxidant-pro-oxidant profiles for hypertension and depression were very similar. However, the expression of inducible HO-1 was specifically altered in depression and was significantly associated with both the prevalence and severity of depressive symptoms.

Our results on altered antioxidant enzyme activities (GR, GPx-1, and SOD-1) in depression are concordant with the existing body of evidence. Decreased activities of SOD-1 have been reported in depression;19,20 however, increased activities of erythrocyte and serum SOD-1 have also been reported;21,22 with antidepressant medication shown to reduce SOD-1 activity to control levels.23 The latter study also reported increased activities of erythrocyte GPx and plasma GR and, likewise, antidepressants significantly affected these parameters. In our study, GR activity was also increased but GPx was decreased. Nevertheless, there is recent very strong evidence that whole blood GPx activity is lower in depressed patients,24 in line with the evidence from Stefanescu and Ciobica20 and further evidence that antidepressants have limited or no effect on enzyme activity.25 There is also a substantial body of evidence that antioxidant defense systems are impaired in hypertension due to decreased activity of SOD-1 and increased, decreased, or unchanged activity of GP-x.26–28 The imbalance in antioxidant enzyme activities may result in increased susceptibility of red blood cells to lipid peroxidation.22 Indeed, increased levels of MDA have been found in both hypertension29 and depression, and antidepressant treatment has been shown to lower lipid peroxidation.21,23,30

Our analysis of the antioxidant-pro-oxidant equilibrium in hypertension and depression reveals similarities between hypertension and depression, with non-specific changes in SOD-1, GR, and GPx-1 activity or peroxide concentration. Inducible HO-1 was specifically altered in depression, with enzyme expression significantly associated with both prevalence and severity of depressive symptoms. The biological effects of the enzyme have been ascribed to its immunomodulatory, anti-inflammatory, and antioxidant functions.31,32 HO catalyzes degradation of heme to biliverdin, iron, and carbon monoxide, and degradation of free heme is important to prevent its cytotoxic and pro-oxidant effects.33 Inducible HO-1 is known to be upregulated in response to pro-oxidant and pro-inflammatory factors including free heme, H2O2, prostaglandins, nitric oxide, peroxynitrite, and pro-inflammatory cytokines.34 The increased levels of H2O2 and MDA in our study indicate a pro-oxidant milieu in depressed and hypertensive patients. The cellular response to excess ROS involves the induction of antioxidant response element (ARE) genes including glutathione-synthesizing glutamate– cysteine ligase and HO-1.35 Here, we observed increased levels of glutathione but decreased expression of HO-1 in depressed patients compared to controls. This observation may be explained by another, ROS-independent mechanism of ARE gene regulation, namely via Bach1. Bach1 is usually bound by its ligand heme, which displaces it from the ARE for export from the nucleus and subsequent degradation. It has been previously been shown that Bach1 de-repression is required prior to Nrf2-dependent HO-1 gene expression; therefore, the Bach1-heme-HO-1 pathway allows coordination of overall intracellular levels of heme and iron with antioxidant protection.36 We have previously reported a strong link between immune function, oxidative stress, and iron homeostasis in the blood of depressed patients;37 however, the molecular mechanism was not explored. Our current results point toward a plausible role for HO-1 in depression, and we hypothesize that deregulation of the Bach1-heme-HO-1 pathway represents a molecular mechanism that might further explain the interplay between immune function, oxidative stress, and iron homeostasis in depression.

The products of heme catabolism also have cytoprotective, anti-apoptotic, antioxidant, and anti-inflammatory properties, suggesting an important role for HO-1 in the resolution of inflammation.38–40 The activity of HO is directly related to cardiovascular function and has a recognized vasoprotective role;14,41 however, little is known about the role of the enzyme in clinical hypertension. Here, no difference in the concentration of HO-1 protein in hypertensive patients and normotensive controls was observed. In contrast, depression was associated with significant down-regulation in both depressed patients and patients with comorbid depression and hypertension.

The relationship between HO and hypertension has been extensively studied but mainly in animal models. HO-1 overexpression is protective of renovascular hypertension in rats, most likely via interference with the action of angiotensin II (AngII).42 The interaction between AngII and HO-1 was further confirmed in a human study in which HO-1 mRNA and protein synthesis and activity was down-regulated in neutrophils from healthy subjects treated with AngII and in cells from hypertensive patients.43 Moreover, HO-1 inhibition was reversed in neutrophils from hypertensive patients treated with anti-hypertensive drugs. The effect of anti-hypertensive treatment might have accounted for the lack of significant changes in HO-1 levels in treated hypertensive patients in our study.

There is little evidence about HO-1 expression and activity in depression; however, the neuroprotective role of the enzyme in neurodegenerative disorders such as Alzheimer's disease has been established.44 Furthermore, recent findings have shown that the neuroprotective effect of the tricyclic antidepressant desipramine was related to increased HO-1 expression,45 and the neuroprotective and anti-neuroinflammatory effects of some herbal medications on hippocampal and microglial cells have been also ascribed to HO activity.46 Taken together, the literature suggests that HO has a neuroprotective role, and since neurodegenerative process might play an important role in depression10 the association between decreased levels of HO and depression reported here can be indirectly linked to neural cell damage. However, HO-1 induction can be associated with both beneficial and deleterious effects depending on the experimental conditions and the neurodegenerative rodent models used.47 Neuroinflammation is a well-known contributor to brain disorders and depression and neurodegenerative diseases in particular. Inflammatory cascades that activate microglia are triggered in response to brain damage such as oxidative stress, and activated microglia are a source of the factors that induce apoptosis and disrupt the blood–brain barrier (BBB). For instance, Zhao et al.48 showed that enhanced expression of HO protected aganist BBB damage in rats.

Conclusions

Here, we present evidence on a potential role for HO as a biological factor that links inflammation, oxidative stress, and the biological and functional changes in brain activity in depression. The identification of decreased HO-1 expression as a potential risk factor for depression in patients with essential hypertension may provide an avenue for preventive strategies against depression in this group of patients. For instance, there may be a rationale for monitoring HO-1 as a biomarker for depression in patients with hypertension. Furthermore, HO in comorbid depression may represent a more universal biological mechanism mediating the development and progression of depressive disorder in general, which is further supported by the fact that psychological stress induces inflammatory and pro-oxidant pathways is often an antecedent to depression. However, neither inflammation nor oxidative stress is sufficient to induce depression; therefore, identification of biological factors specifically related to depression may have important implications for the diagnosis, treatment, and prevention of the disease.

Further research

Our results support the growing body of evidence that, while HO-1 is a critically important cytoprotective molecule, the degree of upregulation in response to an insult may serve as a marker of injury. Further studies should aim to determine whether HO-1 expression plays an antecedent, possibly causal, role in the psychiatric disorder or is solely related to the clinical manifestations in patients, i.e., chronicity and/or the impact of therapy. Whether HO-1 expression can predict the occurrence of depressive symptoms has yet to be validated in epidemiological studies, and a large-scale prospective trial in which the enzyme is measured would be of benefit.

Limitations

Larger, independent studies in medication-free subjects are required in order to translate our results into clinical practice.

Acknowledgement

This study was funded by the research grant awarded to Joanna Rybka from the Ministry of Science and Higher Education.

Disclaimer statements

Contributors None.

Funding None.

Conflict of interest There are no conflicts-of-interest.

Ethics approval Written informed consent was obtained from all the participants of the study. The study was approved by the Nicolaus Copernicus University in Torun Human Ethics Committee and was conducted under the tenets of the Declaration of Helsinki.

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